scholarly journals Effect of fast protein liquid chromatography fractionated salivary gland extracts from different ixodid tick species on interleukin-8 binding to its cell receptors

2003 ◽  
Vol 50 (1) ◽  
pp. 79-84 ◽  
Author(s):  
Paulina Kocakova ◽  
Monika Slavikova ◽  
Valeria Hajnicka ◽  
Mirko Slovak ◽  
Juraj Gasperik ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Salivary Gland ◽  
Tick Species ◽  
Ixodid Tick ◽  
Interleukin 8 ◽  
Fast Protein Liquid Chromatography ◽  
Cell Receptors

In vitro splenocyte proliferation responses of BALB/c mice to salivary gland extracts of three ixodid tick species (Acari: Ixodidae)

Biologia ◽  
2007 ◽  
Vol 62 (6) ◽  
Author(s):  
Mária Kazimírová ◽  
Terézia Rolníková ◽  
Emília Dvorožňáková ◽  
Milan Buc
Keyword(s):  
Salivary Gland ◽  
Cell Cultures ◽  
Tick Species ◽  
Ixodid Tick ◽  
Rhipicephalus Appendiculatus ◽  
Ixodid Ticks ◽  
In Vitro Proliferation ◽  
Murine Splenocytes ◽  
Ifn Γ

AbstractIn vitro proliferation and cytokine production were investigated in BALB/c mice splenic cell cultures that were stimulated with concanavalin A (ConA) and lipopolysaccharide (LPS) and simultaneously exposed to salivary gland extracts (SGE) of unfed and partially fed adult ixodid ticks (Ixodes ricinus, Rhipicephalus appendiculatus, Amblyomma variegatum). Generally, tick SGE enhanced proliferation of unstimulated splenocytes and SGE of unfed ticks suppressed mitogen induced proliferation. Partially fed R. appendiculatus and A. variegatum suppressed ConA responses, while partially fed I. ricinus stimulated both ConA and LPS induced proliferation. A. variegatum and R. appendiculatus females slightly enhanced LPS responses 2 days after attachment but suppressed them at the end of the slow feeding phase. In 72 h ConA induced cell cultures, interferon gamma (IFN-γ) production was suppressed by SGE of all ticks, interleukin (IL)-10 production was enhanced by unfed I. ricinus and partially fed A. variegatum males and IL-5 production was enhanced by feeding R. appendiculatus females and A. variegatum males. The study revealed variability in the responsiveness of murine splenocytes to SGE of different ixodid tick species, whereby patterns of host immunomodulation within one tick species differed between sexes and changed during feeding.

Glutathione S-Transferase Activity in Nontreated and CGA-154281- Treated Maize Shoots

1991 ◽  
Vol 46 (9-10) ◽  
pp. 850-855 ◽  
Author(s):  
John V. Dean ◽  
John W. Gronwald ◽  
Michael P. Anderson
Keyword(s):  
Liquid Chromatography ◽  
Anion Exchange ◽  
Cinnamic Acid ◽  
Fast Protein Liquid Chromatography ◽  
Transferase Activity ◽  
Glutathione S Transferase ◽  
Selective Enhancement

Abstract Fast protein liquid chromatography (anion exchange) was used to separate glutathione S-transferase isozymes in nontreated etiolated maize shoots and those treated with the herbi­cide safener CGA -1542814-(dichloroacetyl)-3,4-dihydro-3-methyl-2 H-1 ,4-benzoxazine. Non­treated shoots contained isozymes active with the following substrates: trans-cinnamic acid (1 isozyme), atrazine (3 isozymes), 1-chloro-2,4-dinitrobenzene (1 isozyme), metolachlor (2 isozymes) and the sulfoxide derivative of S-ethyl dipropylcarbamothioate (2 isozymes). Pre­treatment of shoots with the safener CGA -154281 (1 μM) had no effect on the activity of the isozymes selective for trans-cinnamic acid and atrazine but increased the activity of the constitutively-expressed isozymes that exhibit activity with 1-chloro-2,4-dinitrobenzene, metola­chlor and the sulfoxide derivative of S-ethyl dipropylcarbamothioate. The safener pretreat­ment also caused the appearance of one new isozyme active with 1-chloro-2,4-dinitrobenzene and one new isozyme active with metolachlor. The results illustrate the complexity of gluta­thione S-transferase activity in etiolated maize shoots, and the selective enhancement of gluta­thione S-transferase isozymes by the safener CGA -154281.

scholarly journals Tryptic phosphopeptides from whole casein. I. Preparation and analysis by fast protein liquid chromatography

1989 ◽  
Vol 56 (4) ◽  
pp. 603-611 ◽  
Author(s):  
Marcel A. Juillerat ◽  
Robert Baechler ◽  
Raphael Berrocal ◽  
Serge Chanton ◽  
Jean-Claude Scherz ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Anion Exchange ◽  
Exchange Resin ◽  
Anion Exchange Resin ◽  
Gradient Elution ◽  
Fast Protein Liquid Chromatography ◽  
Parent Species ◽  
Net Charge ◽  
Phosphorylated Peptides ◽  
Salt Gradient

SummaryTryptic phosphopeptides were obtained from whole bovine casein by chromatography on the anion exchange resin QAE-Sephadex A 25. Salt gradient elution of the column allowed separation of non-phosphorylated peptides from phosphorylated species. The preparations obtained contained at least seven distinct phosphopeptides of which the following casein fragments were identified: αs1(43–58):2P, αs1(59–79): 5P, αs2(46–70): 4P, β(1–28): 4P, β(2–28): 4P, and β(33–48): 1P. Fast protein liquid chromatography (FPLC) on Mono Q HR 5/5 resin showed that the phosphopeptides were eluted in the same order as from the QAE-Sephadex resin. However, on the analytical column HR 5/5 the fragments αs1(59–79): 5P and β(2–28): 4P, having the same net charge under the conditions of chromatography, co-eluted, whereas they were at least partly separated on the preparative column HR 16/10. Following enzymic dephosphorylation, the peptides eluted at lower salt strength in the gradient. FPLC on Mono Q resin thus permitted dephosphorylation to be monitored and intermediates between the parent species and the fully dephosphorylated peptide to be identified.

Survey of ixodid tick species on domestic cats in Japan

2003 ◽  
Vol 111 (2-3) ◽  
pp. 231-239 ◽  
Author(s):  
Yojiro Shimada ◽  
Hisashi Inokuma ◽  
Takeshi Beppu ◽  
Masaru Okuda ◽  
Takafumi Onishi
Keyword(s):  
Tick Species ◽  
Ixodid Tick ◽  
Domestic Cats

scholarly journals Small mammals as hosts of immature ixodid ticks

2005 ◽  
Vol 72 (3) ◽  
Author(s):  
I.G. Horak ◽  
L.J. Fourie ◽  
L.E.O. Braack
Keyword(s):  
Small Mammals ◽  
Tick Species ◽  
Ixodid Tick ◽  
Ixodid Ticks ◽  
Free State ◽  
Immature Stages ◽  
Rhabdomys Pumilio ◽  
Haemaphysalis Leachi ◽  
Lepus Capensis ◽  
Multimammate Mice

Two hundred and twenty-five small mammals belonging to 16 species were examined for ticks in Free State, Mpumalanga and Limpopo Provinces, South Africa, and 18 ixodid tick species, of which two could only be identified to genus level, were recovered. Scrub hares, Lepus saxatilis, and Cape hares, Lepus capensis, harboured the largest number of tick species. In Free State Province Namaqua rock mice, Aethomys namaquensis, and four-striped grass mice, Rhabdomys pumilio, were good hosts of the immature stages of Haemaphysalis leachi and Rhipicephalus gertrudae, while in Mpumalanga and Limpopo Provinces red veld rats, Aethomys chrysophilus, Namaqua rock mice and Natal multimammate mice, Mastomys natalensis were good hosts of H. leachi and Rhipicephalus simus. Haemaphysalis leachi was the only tick recovered from animals in all three provinces.

Improved enzyme screening by automated fast protein liquid chromatography

1985 ◽  
Vol 151 (2) ◽  
pp. 547-553 ◽  
Author(s):  
Horst Schütte ◽  
Werner Hummel ◽  
Maria-Regina Kula
Keyword(s):  
Liquid Chromatography ◽  
Fast Protein Liquid Chromatography ◽  
Enzyme Screening
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