scholarly journals Analysis of transcripts of heat shock protein genes in silkworm, Bombyx mori (Lepidoptera: Bombycidae)

2015 ◽  
Vol 112 (4) ◽  
pp. 676-687 ◽  
Author(s):  
Nalavadi CHANDRAKANTH ◽  
Kangayam M. PONNUVEL ◽  
Shunmugam M. MOORTHY ◽  
Sirigineedi SASIBHUSHAN ◽  
Vankadara SIVAPRASAD
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Bombyx Mori ◽  
Heat Shock Protein Genes ◽  
Silkworm Bombyx Mori

Expression of metabolic enzyme genes and heat-shock protein genes during embryonic development in diapause and non-diapause egg of multivoltine silkworm Bombyx mori

Biologia ◽  
2008 ◽  
Vol 63 (5) ◽  
Author(s):  
Ragunathan Saravanakumar ◽  
Kangayam Ponnuvel ◽  
Syed Qadri
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Bombyx Mori ◽  
Early Stage ◽  
Metabolic Enzyme ◽  
Dehydrogenase Gene ◽  
Heat Shock Protein Genes ◽  
High Level ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Silkworm Bombyx Mori

AbstractThe expression of metabolic enzyme genes and heat-shock protein genes (Hsp) during early embryogenesis in diapause and non-diapause eggs of the silkworm Bombyx mori was quantified by semi-quantitative RT-PCR. The trehalase gene (Tre) was expressed in non-diapause eggs up-to nine days, while in diapause eggs was not up regulated. The glycogen phosphorylase gene (GPase) was expressed in non-diapause eggs, whereas in diapause eggs a high level was observed in early stage, but down regulated in later stage. The phosphofructokinase gene (PFK) and sorbitol dehyrogenase-2 gene (SDH-2) expression was fluctuated in non-diapause eggs, whereas in diapause eggs these were expressed only at early stage and not observed in later stage. The glucose-6-phosphate dehydrogenase gene (G6P-DH) in non-diapause eggs was highly expressed during the differentiation phase and decreased in the organogenesis phase. In contrast to this, expression in diapause eggs was of low level during differentiation phase and of high level observed in the organogenesis phase. In the tissues, PFK and SDH-2 were selectively expressed in cuticle and midgut, whereas Tre expression was high in midgut and ovary of larvae incubated at 15°C. The Hsp (20.4, 20.8, 40, 70, and 90) were expressed in both diapause and non-diapause eggs. Their expression was, however, selective in tissues with Hsp20.4 in midgut and ovary, Hsp40 in head, Hsp70 in cuticle and Hsp90 in ovary and head in high amounts at 15°C. These results suggest that the metabolic enzyme genes studied except Hsp play a major role during embryogenesis of diapause and non-diapause silkworm.

scholarly journals The small heat shock protein (sHSP) genes in the silkworm, Bombyx mori, and comparative analysis with other insect sHSP genes

2009 ◽  
Vol 9 (1) ◽  
pp. 215 ◽  
Author(s):  
Zi-Wen Li ◽  
Xue Li ◽  
Quan-You Yu ◽  
Zhong-Huai Xiang ◽  
Hirohisa Kishino ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Bombyx Mori ◽  
Small Heat Shock Protein ◽  
Silkworm Bombyx Mori

scholarly journals Characterization of Heat Shock Protein 60 as an Interacting Partner of Superoxide Dismutase 2 in the Silkworm, Bombyx mori, and Its Response to the Molting Hormone, 20-Hydroxyecdysone

Antioxidants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1385
Author(s):  
Yosui Nojima
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Bombyx Mori ◽  
Molecular Mechanisms ◽  
Heat Shock Protein 60 ◽  
Molting Hormone ◽  
Superoxide Dismutase 2 ◽  
Silkworm Bombyx Mori

Oxidative stress promotes pupation in some holometabolous insects. The levels of superoxide, a reactive oxygen species (ROS), are increased and superoxide dismutase 1 (BmSod1) and superoxide dismutase 2 (BmSod2) are decreased during metamorphic events in silkworm (Bombyx mori). These observations strongly suggest that pupation is initiated by oxidative stress via the down-regulation of BmSod1 and BmSod2. However, the molecular mechanisms underlying ROS production during metamorphic events in silkworm remain unknown. To investigate these molecular mechanisms, the peripheral proteins of BmSod1 and BmSod2 were identified and characterized using dry and wet approaches in this study. Based on the results, silkworm heat shock protein 60 (BmHsp60) was identified as an interacting partner of BmSod2, which belongs to the Fe/MnSOD family. Furthermore, the present study results showed that BmHsp60 mRNA expression levels were increased in response to oxidative stress caused by ultraviolet radiation and that BmHsp60 protein levels (but not mRNA levels) were decreased during metamorphic events, which are regulated by the molting hormone 20-hydroxyecdysone. These findings improve our understanding of the mechanisms by which holometabolous insects control ROS during metamorphosis.

scholarly journals Perturbation of chromatin architecture on ecdysterone induction of Drosophila melanogaster small heat shock protein genes.

1989 ◽  
Vol 9 (1) ◽  
pp. 332-335 ◽  
Author(s):  
S E Kelly ◽  
I L Cartwright
Keyword(s):  
Drosophila Melanogaster ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Intergenic Region ◽  
Small Heat Shock Protein ◽  
Dnase I ◽  
Developmentally Regulated ◽  
Regulatory Interactions ◽  
Chromatin Architecture ◽  
Heat Shock Protein Genes

Alterations in the pattern of DNase I hypersensitivity were observed on ecdysterone-stimulated transcription of Drosophila melanogaster small heat shock protein genes. Perturbations were induced near hsp27 and hsp22, coupled with an extensive domain of chromatin unfolding in the intergenic region between hsp23 and the developmentally regulated gene 1. These regions represent candidates for ecdysterone regulatory interactions.

Sign in / Sign up

Export Citation Format

Share Document