scholarly journals Genetic variation in the diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae) in China inferred from mitochondrial COI gene sequence

2006 ◽  
Vol 103 (3) ◽  
pp. 605-611 ◽  
Author(s):  
Jianhong LI ◽  
Feng ZHAO ◽  
Yong Soo CHOI ◽  
Iksoo KIM ◽  
Hung Dae SOHN ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Plutella Xylostella ◽  
Gene Sequence ◽  
Diamondback Moth ◽  
Coi Gene ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene

scholarly journals Genetic variation among the geographic population of Cassava mealybug Phenacoccus manihoti (Hemiptera: Pseudococcidae) in Indonesia inferred from mitochondrial COI gene sequence

2019 ◽  
Vol 20 (9) ◽  
Author(s):  
Nopriawansyah Nopriawansyah ◽  
AUNU RAUF ◽  
YAYI M. KUSUMAH ◽  
ALI NURMANSYAH ◽  
YONNY KOESMARYONO
Keyword(s):  
Genetic Variation ◽  
Genetic Distance ◽  
Nucleotide Diversity ◽  
Gene Sequence ◽  
Coi Gene ◽  
Phenacoccus Manihoti ◽  
Geographic Population ◽  
Mitochondrial Coi ◽  
Cassava Mealybug ◽  
Mitochondrial Coi Gene

Abstract. Nopriawansyah N, Rauf A, Kusumah YM, Nurmansyah A, Koesmaryono Y. 2019. Genetic variation among the geographic population of Cassava mealybug Phenacoccus manihoti (Hemiptera: Pseudococcidae) in Indonesia inferred from mitochondrial COI gene sequence. Biodiversitas 20: 2685-2692. Cassava mealybug, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae) is an invasive pest native to South America. Research was conducted with the objective to study genetic variation of cassava mealybug from different geographic areas in Indonesia. Thirty-six samples of cassava mealybugs were collected from eight different population locations. Genomic DNA was extracted from individual specimens of P. manihoti using gSYNC DNA Extraction Kit from Geneaid Lot No. FB221707. PCR amplification using primary CO1 gene showed the target position as long as 518 bp. Analysis of homology from eight populations revealed eight haplotypes in Indonesia with different variable sites. Genetic distance analysis was done by calculating base pair differences in each population. The percentage of genetic distance between populations was 0.0000% -0.00409%. The results of the analyst using the Kimura-2 parameter showed that the population was divided into 2 clusters. The highest haplotype (Hd) diversity value was 0.8333, while the lowest was zero (Hd = 0). The highest nucleotide diversity (π) was 0.00225 and the lowest was 0.000000. The genetic distance and nucleotide diversity were low because P. manihoti was thelytoky parthenogenesis.

Classification of Pelteobagrus fish in Poyang Lake based on mitochondrial COI gene sequence

2015 ◽  
Vol 27 (6) ◽  
pp. 4635-4637
Author(s):  
Bin Zhong ◽  
Ting-Ting Chen ◽  
Rui-Yue Gong ◽  
Zhe-Xia Zhao ◽  
Binhua Wang ◽  
...  
Keyword(s):  
Gene Sequence ◽  
Poyang Lake ◽  
Coi Gene ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene

scholarly journals First record of Zaprionus indianus (Gupta, 1970) (Diptera Drosophilidae) from Bangladesh

2011 ◽  
Vol 11 (3) ◽  
pp. 757-760
Author(s):  
Sadniman Rahman ◽  
Deponkor Kumar Roy ◽  
Mohammad Shamimul Alam
Keyword(s):  
Gene Sequence ◽  
First Record ◽  
Coi Gene ◽  
Zaprionus Indianus ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene

Zaprionus indianus (Gupta, 1970) (Diptera Drosophilidae), also known as African fig fly, isconsidered as the primary pest only to fig fruits along with secondary pest to other fruits. Its oc-currence has been reported from many countries but not previously reported from Bangladesh.With this note, we report here the first observation of Z. indianus from Bangladesh. Fly sampleswere collected by using yeast-banana traps. The species was identified by morphological char-acteristics and mitochondrial COI gene sequence.

scholarly journals Genetic identification of Javan hawk-eagle (Nisaetus bartelsi) from Indonesia using mitochondrial COI gene

2019 ◽  
pp. 70-77
Author(s):  
Suhadi ◽  
Dwi Listyorini ◽  
Riri Wiyanti Retnaningtyas ◽  
Fima Rizki Eka Putri ◽  
Dina Ayu Valentiningrum
Keyword(s):  
Phylogenetic Tree ◽  
Gene Sequence ◽  
Dna Isolation ◽  
Genetic Identification ◽  
Coi Gene ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Conservation Effort ◽  
Interspecific Distance ◽  
Intraspecific Distance

Nisaetus bartelsi is a native hawk from Java which its genetic information is not commonly understood yet. As a part of the conservation effort to maintain genetic diversity of this endangered species, this research aimed to obtain COI gene sequences from five individuals of N. bartelsi to confirm its position in the phylogenetic tree. DNA isolation from 5 N. bartelsi blood sample was performed and its COI gene sequence was amplified, sequenced, and used to reconstruct phylogenetic tree using MEGA6 with several other members of Nisaetus, Aquila, and Saggitaridae family. Furthermore, the intraspecific distance between 5 N. bartelsi samples and interspecific distance with other species were calculated using MEGA6. The result suggested that all five individuals belonged to the species Javan hawk-eagle (N. bartelsi) and were closely related to the Blyth’s hawk-eagle (Nisaetus alboniger). The DNA barcoding of the Javan hawk-eagle conducted in this study is a stepping stone to conservation efforts for the Javan hawk-eagle.

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