scholarly journals C-banding, fluorescent staining and NOR location in holokinetic chromosomes of bugs of the Neotropical genus Antiteuchus (Heteroptera: Pentatomidae: Discocephalinae)

2006 ◽  
Vol 103 (1) ◽  
pp. 239-243 ◽  
Author(s):  
Cecilia LANZONE ◽  
Maria Jose DE SOUZA
Keyword(s):  
Fluorescent Staining ◽  
Holokinetic Chromosomes ◽  
C Banding ◽  
Heteroptera Pentatomidae

Karyotypic and chromosome banding studies of the potato tuber moth, Phthorimaea operculella (Zeller) (Lepidoptera, Gelechiidae)

1984 ◽  
Vol 26 (2) ◽  
pp. 141-145 ◽  
Author(s):  
D. G. Bedo
Keyword(s):  
Silver Staining ◽  
Chromosome Banding ◽  
Phthorimaea Operculella ◽  
Holocentric Chromosomes ◽  
Potato Tuber Moth ◽  
Considerable Variability ◽  
Fluorescent Staining ◽  
Hoechst 33258 ◽  
Metaphase Chromosomes ◽  
C Banding

The karyotype of Phthorimaea operculella is similar in both sexes and consists of 29 chromosome pairs. These are of similar size with gradual intergradation except for one pair which is significantly longer. C-banding and fluorescent staining with quinacrine and Hoechst 33258 failed to induce bands in metaphase chromosomes while silver staining clearly showed active nucleoli in all stages except metaphase. The banding results are compared with the few reports available on banding of holocentric chromosomes. It is concluded that considerable variability exists in the heterochromatic structure of holocentric chromosomes.Key words: Lepidoptera, moth, karyotype, C-banding.

A study on the karyotype, Ag-NORs and C-banding inEpinephelus bleekeri

2012 ◽  
Vol 36 (5) ◽  
pp. 647
Author(s):  
Yan CAI ◽  
Yong-can ZHOU ◽  
Rui-min XIE ◽  
Zhen-yu XIE ◽  
Yong-qin FENG ◽  
...  
Keyword(s):  
C Banding

C-banding and fluorescence in situ hybridization studies of the wheat–alien hybrid 'Agrotana'

Genome ◽  
1994 ◽  
Vol 37 (3) ◽  
pp. 477-481 ◽  
Author(s):  
Jie Xu ◽  
R. L. Conner ◽  
A. Laroche
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Genomic Dna ◽  
Chromosome Engineering ◽  
C Banding ◽  
Chinese Spring Wheat ◽  
Mother Cells ◽  
Alien Chromatin ◽  
Chromosome Segments

'Agrotana', a wheat-alien hybrid (2n = 56), is a potential source of resistance to common root rot, stem rust, wheat streak mosaic virus, and the wheat curl mite. However, the origin of 'Agrotana', reported to be durum wheat × Agropyron trichophorum (pubescent wheatgrass), is uncertain. The objective of this investigation was to determine the chromosome constitution of 'Agrotana' using C-banding and fluorescence in situ hybridization techniques. The F1 hybrid of 'Agrotana' × 'Chinese Spring' wheat showed 7 I + 21 II in 14.9% of the pollen mother cells, evidence of the presence of the A, B, and D genomes in 'Agrotana'. The hybrid had 16 heavily C-banded chromosomes, namely 4A, and 1-7B of wheat, and a translocation that probably involved wheat chromosomes 2A and 2D. In situ hybridization using biotinylated genomic DNA of Ag. trichophorum cv. Greenleaf blocked with CS DNA failed to identify the alien chromosomes in 'Agrotana', indicating that the alien chromosomes were not likely derived from pubescent wheatgrass. In situ hybridization using labelled wheat genomic DNA blocked with 'Agrotana' DNA revealed that 'Agrotana' had 40 wheat, 14 alien, and 2 (a pair) wheat–alien translocated chromosomes. There was no homology between wheat and the alien chromosomes or chromosome segments involved in the wheat–alien recombinant. Two of the seven pairs of alien chromosomes were homoeologous to each other. The ability to identify alien chromatin in wheat using labelled wheat DNA instead of labelled alien DNA will be particularly useful in chromosome engineering of wheat germplasms having alien chromatin of unknown origin.Key words: wheat–alien hybrid, C-banding, fluorescence in situ hybridization, labelled wheat DNA as probe.

scholarly journals Development and reproduction of Podisus distinctus (Heteroptera: Pentatomidae) fed on larva of Bombyx mori (Lepidoptera: Bombycidae)

2004 ◽  
Vol 64 (2) ◽  
pp. 237-242 ◽  
Author(s):  
M. C. Lacerda ◽  
A. M. R. M. Ferreira ◽  
T. V. Zanuncio ◽  
J. C. Zanuncio ◽  
A. S. Bernardino ◽  
...  
Keyword(s):  
Biological Control ◽  
Life Cycle ◽  
Relative Humidity ◽  
Bombyx Mori ◽  
Insect Pests ◽  
Males And Females ◽  
Heteroptera Pentatomidae ◽  
Bombyx Mori L ◽  
Chemical Products ◽  
Coleoptera Tenebrionidae

Biological control has been reducing the use of chemical products against insect pests, specially predatory Pentatomidae. Species of this group can present high variations in their life cycle as a result of their diet. Thus, the objective of this research was to study nymph development and reproduction of Podisus distinctus (Stäl, 1860) (Heteroptera: Pentatomidae) fed on Bombyx mori L., 1758 (Lepidoptera: Bombycidae) larvae (T1), compared to those fed on Tenebrio molitor L., 1758 (Coleoptera: Tenebrionidae) (T2) and Musca domestica L., 1758 (Diptera: Muscidae) larvae (T3) at a temperature of 25 ± 0.5ºC, relative humidity of 70 ± 2%, and photophase of 12 h. Predators fed on B. mori showed duration of the nymph phase (18.68 ± 1.02) similar to those fed on T. molitor (18.32 ± 1.49). Pre-oviposition and oviposition periods and number of egg masses, besides eggs and nymphs per female, were higher with B. mori (5.83 ± 2.02; 15.00 ± 7.40; 8.42 ± 1.84; 296.69 ± 154.75; and 228.55 ± 141.04, respectively) while longevity of males and females of P. distinctus was 25.76 ± 16.15 and 35.00 ± 16.15 days with T. molitor, and 20.57 ± 13.60 and 23.46 ± 12.35 days with B. mori, respectively.

Is Elsiella Froeschner, 1981 a valid genus? (Hemiptera: Heteroptera: Pentatomidae: Pentatominae)

Zootaxa ◽  
2012 ◽  
Vol 3238 (1) ◽  
pp. 39 ◽  
Author(s):  
FELIPE LORENZ SIMÕES ◽  
AUGUSTO FERRARI ◽  
JOCÉLIA GRAZIA
Keyword(s):  
Cladistic Analysis ◽  
Morphological Characters ◽  
Data Set ◽  
Male Specimen ◽  
Valid Genus ◽  
Heteroptera Pentatomidae

The genus Elsiella Froeschner, 1981 is validated based on the analysis of a recently found male specimen of E. plana(Walker, 1867). A cladistic analysis comprising a data set of 40 morphological characters and 22 taxa, including the generaElsiella, Serdia Stål, Similliserdia Fortes & Grazia, Neotibilis Grazia & Barcellos, and Tibilis Stål, was performed. Elsiellaplana is redescribed and illustrated. Maps are provided for Elsiella and Serdia with biogeographical considerations for Serdia.

Annual Cycle of Freshwater Diatoms in the High Arctic Revealed by Multiparameter Fluorescent Staining

2020 ◽  
Vol 80 (3) ◽  
pp. 559-572
Author(s):  
Eva Hejduková ◽  
Josef Elster ◽  
Linda Nedbalová
Keyword(s):  
Annual Cycle ◽  
High Arctic ◽  
Fluorescent Staining ◽  
Freshwater Diatoms
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