scholarly journals Interaction of antibodies from the sera of autoimmune patients thyroidopathies with isolated diffuse cells toxic euthyroid nodular goiter.

1993 ◽  
Vol 39 (6) ◽  
pp. 46-50 ◽  
Author(s):  
S. I. Krainova ◽  
V. I. Kandror
Keyword(s):  
Enzyme Immunoassay ◽  
Surface Antigen ◽  
Indirect Immunofluorescence ◽  
Solid Phase ◽  
Nodular Goiter ◽  
Surface Antigens ◽  
Tissue Samples ◽  
Toxic Goiter ◽  
Antimicrosomal Antibody ◽  
Antibody Population

Blood sera of 46 patients with diffuse toxic goiter (DTG) and of 48 ones with Hashimotos thyroiditis (HT) were tested for antibodiescomplement-mediated cytotoxicity carriers (ACMMC). ACMCC targets were isolated DTG cells and cells of euthyroid nodular goiter (ENG) perinodular tissue. Antimicrosomal antibodies were assayed in the sera by indirect immunofluorescence and antibodies to all thyrocyte surface antigens isolated from both tissue samples were determined by solid-phase enzyme immunoassay. When DTG cells were targets, DTG patients' sera detected ACMCC in 36 % of cases and HT patients sera in 73% of cases (p0.001). In ENG cells the sera of patients of both groups detected ACMCC equally frequently (in more than 70% of cases). Of the 27 DTG patients sera tested with both tissues app. roximately a half detected ACMCC in only ENG tissue. There was no difference in HT patients sera effects on ACMCC detection in both tissue samples. This has brought the authors to a conclusion about DTG cells deficiency for ACMCC mediating antigens. Moreover, DTG cells bound much less antibodies from sera of patients with autoimmune thyropathies, than ENG cells (p0.001), this confirming a deficiency of surface antigen on DTG cells. No correlation between the presence in the sera of antimicrosomal cells and of ACMCC was detected. A conclusion has been made about heterogeneity of antimicrosomal antibody population and about the presence of ACMCC in blood sera of patients with autoimmune thyropathies, these antibodies not belonging to antimicrosomal ones. ACMCC also may be heterogenous and differ in DTG and HT patients.

scholarly journals Solid-phase enzyme immunoassay for immunoglobulin M antibodies to cytomegalovirus

1977 ◽  
Vol 5 (6) ◽  
pp. 629-634
Author(s):  
H Schmitz ◽  
H W Doerr ◽  
D Kampa ◽  
A Vogt
Keyword(s):  
Alkaline Phosphatase ◽  
Horseradish Peroxidase ◽  
Enzyme Immunoassay ◽  
Indirect Immunofluorescence ◽  
Solid Phase ◽  
Immunoglobulin M ◽  
Nuclear Antigen ◽  
Immunofluorescence Method ◽  
Infected Cells

A sensitive, solid-phase enzyme immunoassay for the detection of immunoglobulin M antibodies to cytomegalovirus is described. The results of the enzyme immunoassay correlated well with those obtained by an indirect immunofluorescence method. Horseradish peroxidase proved to be a more sensitive label than alkaline phosphatase. Nonspecific reactions, occurring with commercially available cytomegalovirus antigens, could be avoided by using a nuclear antigen prepared from sonically disrupted nuclei of cytomegalovirus-infected cells.

scholarly journals Solid-phase enzyme-immunoassay for detection of hepatitis B surface antigen.

1976 ◽  
Vol 29 (10) ◽  
pp. 873-879 ◽  
Author(s):  
G Wolters ◽  
L Kuijpers ◽  
J Kacaki ◽  
A Schuurs
Keyword(s):  
Hepatitis B ◽  
Enzyme Immunoassay ◽  
Surface Antigen ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen

Solid-phase enzyme immunoassay for hepatitis B surface antigen.

1977 ◽  
Vol 23 (5) ◽  
pp. 813-815 ◽  
Author(s):  
R Wei ◽  
G J Knight ◽  
D H Zimmerman ◽  
H E Bond
Keyword(s):  
Hepatitis B ◽  
Horseradish Peroxidase ◽  
Enzyme Immunoassay ◽  
Surface Antigen ◽  
Room Temperature ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen ◽  
Solid Support ◽  
Controlled Pore Glass ◽  
Pore Glass

Abstract A solid-phase enzyme immunoassay is described for measuring hepatitis B surface antigen in human serum or plasma. Immunologically purified antibody labeled with horseradish peroxidase was used as the indicator. In the assay system, antibody-coated controlled-pore glass is used as a solid support and there are three sequential incubations, totaling 2 h, at room temperature. Results for serially diluted positive and reference sera compare favorably to radioimmunoassay in sensitivity and specificity.

A solid-phase enzyme immunoassay for the common and subtypic determinants of hepatitis B surface antigen with monoclonal antibodies

1986 ◽  
Vol 87 (2) ◽  
pp. 203-210 ◽  
Author(s):  
Sadakazu Usuda ◽  
Fumio Tsuda ◽  
Tohru Gotanda ◽  
Katsumi Tachibana ◽  
Motozumi Nomura ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Hepatitis B ◽  
Enzyme Immunoassay ◽  
Surface Antigen ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen ◽  
The Common

A monoclonal-antibody enzyme immunoassay for detection of hepatitis B surface antigen with use of a biotin-avidin system.

1985 ◽  
Vol 31 (2) ◽  
pp. 202-205 ◽  
Author(s):  
Y S Liu ◽  
A Green
Keyword(s):  
Hepatitis B ◽  
Enzyme Immunoassay ◽  
Surface Antigen ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen ◽  
Standard Procedure ◽  
Complex Form ◽  
Antibody Complex ◽  
Covalently Linked ◽  
Viral Determinants

Abstract In this solid-phase two-site enzyme immunoassay for hepatitis B surface antigen (HBsAg), three monoclonal anti-HBs are used: 5D3 (IgM) is immobilized on plastic beads; 5C3 (IgG2a) and 5C 11 (IgG1), labeled with biotin, are used as the first conjugate. Horseradish peroxidase covalently linked to avidin is the second conjugate. First, serum or plasma is incubated with the antibody-coated bead and biotin-labeled antibodies, simultaneously, at 45 degrees C for 1 h ("stat" procedure), 3 h ("standard" procedure), or 18 h ("overnight" procedure), during which HBsAg forms a complex with the solid-phase antibody and the biotinylated antibodies. The enzyme-conjugated avidin is then bound to the biotin on the antigen-antibody complex at 45 degrees C for 15 min ("stat") or 30 min (standard and overnight procedures). The beads are incubated with enzyme-substrate solution (H2O2 and o-phenylenediamine). Color developed is measured at 492 nm. All procedures satisfied third-generation HBsAg tests required by the FDA Office of Biologics, being sensitive both to ad and ay subtypes in subnanogram amounts. The assay is reactive with adw2, adw4, adr, ayw2, ayw3, and ayr subtypes and can detect viral determinants in HBsAg-anti-HBs immune complex form. Thus it provides a sensitive, simple, and reproducible alternative to radioimmunoassay.

An "antibody electrode," preliminary report on a new approach in enzyme immunoassay.

1979 ◽  
Vol 25 (2) ◽  
pp. 318-321 ◽  
Author(s):  
J L Boitieux ◽  
G Desmet ◽  
D Thomas
Keyword(s):  
Enzyme Immunoassay ◽  
Surface Antigen ◽  
Solid Phase ◽  
Biological Fluids ◽  
Hepatitis B Surface Antigen ◽  
Antigen Concentration ◽  
New Approach ◽  
Solid Phase Radioimmunoassay ◽  
Reliable Procedure ◽  
Sensitive Electrode

Abstract We report preliminary experiments on a new, sensitive, and reliable procedure for enzyme immunoassay of various antigens in biological fluids. The method, developed from the biological model "hepatitis B surface-antigen/antibodies," is less time consuming than most immunochemical techniques and eliminates many inconveniences arising from use of isotopes. We use a solid-phase "sandwich" procedure, the antibodies being immobilized on gelatin membranes, and determine antigen concentration with the help of an iodide-sensitive electrode modified by fixing the active membrane onto the crystal sensor. We have established the analytical criteria of the method and compared it with the solid-phase radioimmunoassay for the surface antigen in dilution series. One-tenth microgram of antigen per liter can be reproducibly detected with our method. Use of antibody electrode should easily be extended to assay of other antigens and haptens that usually are determined by radioimmunoassay.

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