scholarly journals The role of hormonal-cytokine interactions in the formation of humoral immune response

1995 ◽  
Vol 41 (1) ◽  
pp. 32-34
Author(s):  
S. V. Shirshev
Keyword(s):  
Immune Response ◽  
Chorionic Gonadotropin ◽  
Humoral Immune Response ◽  
Alpha Interferon ◽  
Type I ◽  
Humoral Immune ◽  
Beta Interferon ◽  
Cytokine Interactions

The functional activity of splenocytes of CBA mice was investigated in a syngeneic transfer system by the level of formation of antibody-producing cells (APC). Splenocytes were preincubated for 1 h in vitro with chorionic gonadotropin and type I recombinant interferons, as well as in hormonal-cytokine combinations. Chorionic gonadotropin in doses 10 and 50 MU/ml depressed APC formation, whereas alpha-interferon (250 MU/ml) stimulated it, and beta-interferon in the same concentration did not influence the level of humoral immune response. Chorionic gonadotropin, if added to splenocyte culture in combination with alpha-interferon, completely lost its immunodepressive properties. However, if together with alpha-interferon in a dose of 10 MU/ml it had a costimulating effect, in contrast to that, in combination with beta-interferon in the tested concentrations it was conducive to only a statistically reliable increase in the number of APC.

scholarly journals Role of Bordetella bronchisepticaFimbriae in Tracheal Colonization and Development of a Humoral Immune Response

2000 ◽  
Vol 68 (4) ◽  
pp. 2024-2033 ◽  
Author(s):  
Seema Mattoo ◽  
Jeff F. Miller ◽  
Peggy A. Cotter
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Limit Of Detection ◽  
Immunoglobulin M ◽  
Tissue Culture Cell ◽  
Wild Type ◽  
Humoral Immune ◽  
Tract Infections

ABSTRACT Fimbriae are filamentous, cell surface structures which have been proposed to mediate attachment of Bordetella species to respiratory epithelium. Bordetella bronchiseptica has four known fimbrial genes: fim2, fim3,fimX, and fimA. While these genes are unlinked on the chromosome, their protein products are assembled and secreted by a single apparatus encoded by the fimBCD locus. ThefimBCD locus is embedded within the fha operon, whose genes encode another putative adhesin, filamentous hemagglutinin (FHA). We have constructed a Fim− B. bronchiseptica strain, RB63, by introducing an in-frame deletion extending from fimB through fimD. Western blot analysis showed that RB63 is unable to synthesize fimbriae but is unaffected for FHA expression. Using this mutant, we assessed the role of fimbriae in pathogenesis in vitro and in vivo in natural animal hosts. Although RB63 was not significantly defective in its ability to adhere to various tissue culture cell lines, including human laryngeal HEp-2 cells, it was considerably altered in its ability to cause respiratory tract infections in rats. The number of ΔfimBCD bacteria recovered from the rat trachea at 10 days postinoculation was significantly decreased compared to that of wild-type B. bronchiseptica and was below the limit of detection at 30 and 60 days postinoculation. The number of bacteria recovered from the nasal cavity and larynx was not significantly different between RB63 and the wild-type strain at any time point. The ability of fimbriae to mediate initial attachment to tracheal tissue was tested in an intratracheal inoculation assay. Significantly fewer RB63 than wild-type bacteria were recovered from the tracheas at 24 h after intratracheal inoculation. These results demonstrate that fimbriae are involved in enhancing the ability of B. bronchiseptica to establish tracheal colonization and are essential for persistent colonization at this site. Interestingly, anti-Bordetella serum immunoglobulin M (IgM) levels were significantly lower in animals infected with RB63 than in animals infected with wild-type B. bronchiseptica at 10 days postinoculation. Even at 30 days postinoculation, RB63-infected animals had lower serum anti-Bordetella antibody titers in general. This disparity in antibody profiles suggests that fimbriae are also important for the induction of a humoral immune response.

Role of Autorosette Forming Cells in Antibody Synthesis in vitro: Suppressive Activity of ARFC in Humoral Immune Response

Immunobiology ◽  
1984 ◽  
Vol 166 (1) ◽  
pp. 1-11 ◽  
Author(s):  
N. Khansari ◽  
M. Petrini ◽  
F. Ambrogii ◽  
P. Goldschmidt-Clermont ◽  
H.H. Fudenberg
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Suppressive Activity ◽  
Antibody Synthesis ◽  
Humoral Immune

Colloidal manganese salt improves the efficacy of rabies vaccines in mice, cats, and dogs

2021 ◽  
Author(s):  
Zongmei Wang ◽  
Yueming Yuan ◽  
Chen Chen ◽  
Chengguang Zhang ◽  
Fei Huang ◽  
...  
Keyword(s):  
Immune Response ◽  
B Cells ◽  
Humoral Immune Response ◽  
Plasma Cells ◽  
Vaccine Development ◽  
Type I ◽  
Humoral Immune ◽  
Tfh Cells

Rabies, caused by rabies virus (RABV), remains a serious threat to public health in most countries worldwide. At present, the administration of rabies vaccines has been the most effective strategy to control rabies. Herein, we evaluate the effect of colloidal manganese salt (Mn jelly, MnJ) as an adjuvant of rabies vaccine in mice, cats, and dogs. The results showed that MnJ promoted type I interferon (IFN-I) and cytokine production in vitro and the maturation of dendritic cells (DCs) in vitro and in vivo . Besides, MnJ serving as an adjuvant for rabies vaccines could significantly facilitate the generation of T follicular helper (Tfh) cells, germinal center (GC) B cells, plasma cells (PCs), and RABV-specific antibody-secreting-cells (ASCs), consequently improved the immunogenicity of rabies vaccines and provide better protection against virulent RABV challenge. Similarly, MnJ enhanced the humoral immune response in cats and dogs as well. Collectively, our results suggest that MnJ can facilitate the maturation of DCs during rabies vaccination, which can be a promising adjuvant candidate for rabies vaccines. IMPORTANCE Extending humoral immune response by using adjuvants is an important strategy for vaccine development. In this study, a novel adjuvant MnJ supplemented in rabies vaccines was evaluated in mice, cats, and dogs. Our results in the mouse model revealed that MnJ increased the numbers of mature DCs, Tfh cells, GC B cells, PCs, and RABV-specific ASCs, resulting in enhanced immunogenicity and protection rate of rabies vaccines. We further found MnJ had the same stimulative effect in cats and dogs. Our study provides the first evidence that MnJ serving as a novel adjuvant of rabies vaccines can boost immune response both in a mouse and pet model.

Role of Yersinia pseudotuberculosis outer proteins (Yops) in murine humoral immune response

2009 ◽  
Vol 54 (3) ◽  
pp. 239-245 ◽  
Author(s):  
J. M. L. Maia ◽  
L. G. S. Monnazzi ◽  
B. M. M. Medeiros
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Yersinia Pseudotuberculosis ◽  
Humoral Immune

The role of anti‐flavivirus humoral immune response in protection and pathogenesis

2020 ◽  
Vol 30 (4) ◽  
Author(s):  
Arianna Mahely Hurtado‐Monzón ◽  
Carlos Daniel Cordero‐Rivera ◽  
Carlos Noe Farfan‐Morales ◽  
Juan Fidel Osuna‐Ramos ◽  
Luis Adrián De Jesús‐González ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Humoral Immune

scholarly journals Potential role of soluble CD40 in the humoral immune response impairment of uraemic patients

Immunology ◽  
2003 ◽  
Vol 110 (1) ◽  
pp. 131-140 ◽  
Author(s):  
Cecile Contin ◽  
Vincent Pitard ◽  
Yahsou Delmas ◽  
Nadege Pelletier ◽  
Thierry Defrance ◽  
...  
Keyword(s):  
Immune Response ◽  
Humoral Immune Response ◽  
Potential Role ◽  
Humoral Immune
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