Insulinreceptor interactions and EPR spectroscopy in experimental diabetes mellitus

2019 ◽  
Vol 43 (3) ◽  
pp. 48-50
Author(s):  
N. P. Mikaelyan ◽  
Yu. A. Knyazev ◽  
A. G. Maxina ◽  
B. A. Dainyak ◽  
A. Ye. Gurina ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Epr Spectroscopy ◽  
Plasma Membranes ◽  
Experimental Diabetes ◽  
Inverse Correlation ◽  
Insulin Binding ◽  
Binding Activity ◽  
Insulin Concentration ◽  
Experimental Diabetes Mellitus ◽  
In Cells

Insulin concentration in the blood and the level of its binding in tissues inversely correlate in experimental diabetes mellitus. Insulin-binding activity in cells and the microviscosity and hydrophilism of plasma membranes are in inverse correlation. ATP modifies insulin reception.

scholarly journals THE ALTERATION OF COMPOSITION OF FATTY ACIDS AND INSULIN-BINDING ACTIVITY OF CELLS IN CONDITIONS OF OXIDATIVE STRESS UNDER EXPERIMENTAL DIABETES MELLITUS

2017 ◽  
Vol 23 (6) ◽  
pp. 308-311
Author(s):  
Nina P. Mikaelyan ◽  
A. E Gurina ◽  
A. A Terentev ◽  
A. A Mikaelyan
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Fatty Acids ◽  
Fatty Acid ◽  
Unsaturated Fatty Acids ◽  
Experimental Diabetes ◽  
Saturated Fatty Acids ◽  
Insulin Binding ◽  
Binding Activity ◽  
Experimental Diabetes Mellitus

The parallel study of effect of experimental diabetes mellitus on the level of lipids and composition of fatty acids in blood and homogenates of liver and insulin-binding activity of cells in conditions of oxidative stress demonstrated that in rats already at early period of development of diabetes, alongside with hyperlipoproteinemia, hypertriglyceridemia and hypercholesterolemia significant alterations are marked in structure of fatty acids and homogenates of liver. These alterations are accompanied by peroxide oxidation of lipids and decreasing of activity of enzymes-antioxidants and also by decreasing of insulin-binding activity of cells. Under experimental diabetes mellitus, a significant increasing of level of saturated fatty acids, especially palmitic fatty acid results in decreasing of levels of mono-unsaturated fatty acids (at the expense of oleic fatty acid) that significantly differs corresponding specter in patients with diabetes. The increasing of concentration of eicosapentaenoic and docosahexaenoic acids is accompanied by decreasing of total content of fatty acid Omega-3 at the expense of alfa-linoleic fatty acid. At that, the level of fatty acid Omega-6 reliably decreases both in erythrocytes and homogenates of liver. The results of study also testify that already at early stages of development of diabetes mellitus alterations in pool of fatty acids of erythrocytes and homogenates of liver are similar (with slight differences) and in all analyzed tissues disorder of metabolism of fatty acids has an anti-sclerotic direction.

Effect of Curcuma longa and Galega orientalis on renal function in rats with experimental diabetes mellitus and acute renal failure

2019 ◽  
pp. 88-95
Author(s):  
Р.И. Айзман ◽  
А.П. Козлова ◽  
Е.И. Гордеева ◽  
М.С. Головин ◽  
Г.А. Корощенко ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Renal Failure ◽  
Renal Function ◽  
Acute Renal Failure ◽  
Experimental Diabetes ◽  
Curcuma Longa ◽  
Diabetic Rats ◽  
Standard Diet ◽  
Experimental Diabetes Mellitus ◽  
Galega Orientalis

Цель - исследование влияния куркумы длинной и галеги восточной на осмо- и ионорегулирующую функции почек крыс при аллоксан-индуцированном сахарном диабете и острой почечной недостаточности в эксперименте. Методика. Эксперименты выполнены на самцах крыс Wistar (n=70) с моделью сахарного диабета (1-я серия) и острой почечной недостаточности (2-я серия). В обеих сериях животные были поделены на 3 группы: крыс 1-й группы содержали на стандартном корме, крысам остальных групп в корм добавляли куркуму (2-я группа) или галегу (3-я группа) (2% от массы корма). На 7-е сут эксперимента проводили исследование диуретической и ионоуретической функций почек натощак и после 5% водной нагрузки. Концентрацию ионов в моче и плазме определяли методом пламенной фотометрии; осмотическую концентрацию биологических жидкостей - методом криоскопии; биохимические показатели крови - колориметрическим методом. Результаты. У животных с сахарным диабетом фоновый диурез, а также экскреция натрия и калия были статистически значимо выше, чем у контрольных животных. При острой почечной недостаточности наблюдался более низкий уровень диуреза и ионоуреза, особенно после водной нагрузки. Прием куркумы и галеги вызывал улучшение осмо- и ионорегулирующей функции почек у крыс с сахарным диабетом, и практически не влиял на эти функции почек при острой почечной недостаточности. Заключение. При сахарном диабете оба фитопрепарата вызывали понижение концентрации глюкозы, креатинина, мочевины и улучшение ионно-осмотических показателей плазмы крови, при этом эффект куркумы был выражен отчетливее. При острой почечной недостаточности эти фитопрепараты не давали описанного эффекта. Aim. To study effects of the phytomedicines, Curcuma longa and Galega orientalis, on osmosis- and ion-regulating renal functions in rats with experimental diabetes mellitus (DM) and acute renal failure (ARF). Methods. Experiments were performed in two series on Wistar male rats (n=70) with modeled diabetes mellitus (series 1) and acute renal failure (series 2). In each series, the animals were divided into 3 groups, 1) rats of group 1 receiving a standard diet; 2) rats of groups 2 and 3 receiving a standard diet supplemented with turmeric or galega (2% of food weight), respectively. On the 7th day of the experiment, the diuretic and ionuretic renal function was studied in fasting state and after 5% water loading. Concentrations of ions in urine and plasma were determined by flame photometry; osmotic concentrations of biological fluids were measured by cryoscopy; blood biochemical parameters were measured by colorimetry. Results. In diabetic rats, background diuresis and sodium and potassium excretion were significantly higher than in the control animals. In rats with acute renal failure, diuresis and ionuresis were significantly lower, particularly after the water loading. Turmeric and galega supplementation improved the osmotic and ion-regulating renal function in diabetic rats and left practically unchanged these functions in rats with acute renal failure. Conclusion. In rats with diabetes mellitus, both herbal remedies reduced concentrations of glucose, creatinine, and urea and improved ion-osmotic parameters of blood plasma with a more pronounced effect of turmeric. In acute renal failure, these phytomedicines did not produce the described effects.

MORPHOFUNCTIONAL CHANGES IN THE LIVER OF 24-MONTH-OLD RATS IN THE EARLY STAGES OF EXPERIMENTAL DIABETES MELLITUS DEVELOPMENT

2018 ◽  
Vol 14 (65) ◽  
pp. 152
Author(s):  
O. Ya. Zhurakivska ◽  
Yu. V. Bodnarchuk ◽  
V. M. Pertsovych ◽  
H. B. Kulynych ◽  
V. A. Мiesoiedova
Keyword(s):  
Diabetes Mellitus ◽  
Experimental Diabetes ◽  
Experimental Diabetes Mellitus ◽  
Early Stages ◽  
Old Rats
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