scholarly journals Optimizing Inflammatory Bowel Disease for Successful In Vitro Fertilization

2016 ◽  
Vol 3 (1) ◽  
pp. e199
Author(s):  
Jason J. Pan ◽  
Mora Cannon ◽  
Edward Kane ◽  
Gauree Konijeti
Keyword(s):  
Inflammatory Bowel Disease ◽  
In Vitro Fertilization ◽  
Bowel Disease ◽  
Vitro Fertilization ◽  
Inflammatory Bowel

scholarly journals In Vitro Fertilization in Women With Inflammatory Bowel Disease Is as Successful as in Women From the General Infertility Population

2015 ◽  
Vol 13 (9) ◽  
pp. 1641-1646.e3 ◽  
Author(s):  
Sveta Shah Oza ◽  
Vikas Pabby ◽  
Laura E. Dodge ◽  
Vasiliki A. Moragianni ◽  
Michele R. Hacker ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
In Vitro Fertilization ◽  
Bowel Disease ◽  
Vitro Fertilization ◽  
Inflammatory Bowel

scholarly journals Factors Associated with the Success of In Vitro Fertilization in Women with Inflammatory Bowel Disease

2016 ◽  
Vol 61 (8) ◽  
pp. 2381-2388 ◽  
Author(s):  
Sveta Shah Oza ◽  
Vikas Pabby ◽  
Laura E. Dodge ◽  
Michele R. Hacker ◽  
Janis H. Fox ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
In Vitro Fertilization ◽  
Bowel Disease ◽  
Vitro Fertilization ◽  
Factors Associated ◽  
Inflammatory Bowel

scholarly journals 344 In-Vitro Fertilization in Women With Inflammatory Bowel Disease is as Successful as in Women From the General Infertility Population

2014 ◽  
Vol 146 (5) ◽  
pp. S-79-S-80
Author(s):  
Sveta Shah ◽  
Vikas Pabby ◽  
Laura E. Dodge ◽  
Vasiliki A. Moragianni ◽  
Janis H. Fox ◽  
...  
Keyword(s):  
Inflammatory Bowel Disease ◽  
In Vitro Fertilization ◽  
Bowel Disease ◽  
Vitro Fertilization ◽  
Inflammatory Bowel

P140 3D BIOENGINEERED TISSUE MODEL OF THE LARGE INTESTINE TO STUDY INFLAMMATORY BOWEL DISEASE

2020 ◽  
Vol 26 (Supplement_1) ◽  
pp. S34-S35
Author(s):  
Terrence Roh ◽  
Ying Chen ◽  
Harry Paul ◽  
Chengchen Guo ◽  
David Kaplan
Keyword(s):  
Inflammatory Bowel Disease ◽  
Large Intestine ◽  
Intestinal Epithelium ◽  
Bowel Disease ◽  
Tissue Model ◽  
Inflammatory Bowel ◽  
Silk Scaffold ◽  
Subepithelial Layer ◽  
Migration Of Macrophages

Abstract An in vitro model of intestine epithelium with an immune compartment was bioengineered to mimic immunologic responses seen in inflammatory bowel disease [1]. While aspects of intestinal immunity can be modeled in transwells and 2D culture systems, 3D tissue models improve physiological relevance by providing a 3D substrate which enable migration of macrophages towards the epithelium. An intestinal epithelium comprised of non-transformed human colon organoid cells and a subepithelial layer laden with monocyte-derived macrophages was bioengineered to mimic native intestinal mucosa cell organization using spongy silk scaffolds. Confluent epithelial monolayers with microvilli, a mucus layer, and infiltration of macrophages to the basal side of the epithelium were observed. Inflammation, induced by E. coli O111:B4 lipopolysaccharide and interferon γ resulted in morphology changes to the epithelium, resulting in ball-like structures, decreased epithelial coverage, and migration of macrophages to the epithelium. Analysis of cytokines present in the inflamed tissue model demonstrated significantly upregulated secretion of pro-inflammatory cytokines associated with active inflammatory bowel disease, including CXCL10, IL-1β, IL-6, MCP-2, and MIP-1β. The macrophage layer enhanced epithelial and biochemical responses to inflammatory stimuli, and this new tissue system may be useful to study and develop potential therapies for inflammatory bowel disease. References: 6 Roh, T.T., et al., 3D bioengineered tissue model of the large intestine to study inflammatory bowel disease. Biomaterials, 2019: p. 119517. 7 In, J., et al., Enterohemorrhagic Escherichia coli reduce mucus and intermicrovillar bridges in human stem cell-derived colonoids. Cellular and molecular gastroenterology and hepatology, 2015. 2(1): p. 48–62.e3. 8 Chen, Y., et al., In vitro enteroid-derived three-dimensional tissue model of human small intestinal epithelium with innate immune responses. PLoS ONE, 2017. 12(11): p. e0187880. Colonoid and macrophage cultivation scheme in the 3D bilayer system. (A) Human monocytes were isolated from whole blood and human colonoids from large intestine biopsies were cultured according to established protocols [2]. (B) Cell suspensions of colonoids were seeded on the film surface on the inner silk scaffold and monocyte-derived macrophages were seeded throughout the porous outer silk scaffold using established protocols [3]. (C) The model is cultured for 3 weeks total with 2 weeks in High WNT media and 1 week in differentiation media based on established protocol. Colonoids are present in the model throughout the 3 week culture time. 2 sets of macrophages are added with the first set added after the first week of culture and the second set replacing the first set after the second week.

scholarly journals Food and Food Groups in Inflammatory Bowel Disease (IBD): The Design of the Groningen Anti-Inflammatory Diet (GrAID)

Nutrients ◽  
2021 ◽  
Vol 13 (4) ◽  
pp. 1067
Author(s):  
Marjo J. E. Campmans-Kuijpers ◽  
Gerard Dijkstra
Keyword(s):  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Food Group ◽  
Dietary Guidelines ◽  
Current Evidence ◽  
Group Level ◽  
Food Groups ◽  
Inflammatory Bowel ◽  
Anti Inflammatory

Diet plays a pivotal role in the onset and course of inflammatory bowel disease (IBD). Patients are keen to know what to eat to reduce symptoms and flares, but dietary guidelines are lacking. To advice patients, an overview of the current evidence on food (group) level is needed. This narrative review studies the effects of food (groups) on the onset and course of IBD and if not available the effects in healthy subjects or animal and in vitro IBD models. Based on this evidence the Groningen anti-inflammatory diet (GrAID) was designed and compared on food (group) level to other existing IBD diets. Although on several foods conflicting results were found, this review provides patients a good overview. Based on this evidence, the GrAID consists of lean meat, eggs, fish, plain dairy (such as milk, yoghurt, kefir and hard cheeses), fruit, vegetables, legumes, wheat, coffee, tea and honey. Red meat, other dairy products and sugar should be limited. Canned and processed foods, alcohol and sweetened beverages should be avoided. This comprehensive review focuses on anti-inflammatory properties of foods providing IBD patients with the best evidence on which foods they should eat or avoid to reduce flares. This was used to design the GrAID.

Sign in / Sign up

Export Citation Format

Share Document