scholarly journals Detection of viable hookworm ova from wastewater and sludge

2017 ◽  
Author(s):  
Pradip Gyawali
Keyword(s):  
Hookworm Ova

scholarly journals Rapid concentration and sensitive detection of hookworm ova from wastewater matrices using a real-time PCR method

2015 ◽  
Vol 159 ◽  
pp. 5-12 ◽  
Author(s):  
P. Gyawali ◽  
J.P.S. Sidhu ◽  
W. Ahmed ◽  
P. Jagals ◽  
S. Toze
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Sensitive Detection ◽  
Pcr Method ◽  
Hookworm Ova

A DILUTION—FLOTATION TECHNIC FOR COUNTING HOOKWORM OVA IN FIELD SURVEYS*

1926 ◽  
Vol 6 (supp1) ◽  
pp. 146-159
Author(s):  
FRED C. CALDWELL ◽  
ELFREDA L. CALDWELL
Keyword(s):  
Field Surveys ◽  
Hookworm Ova

Quantification of hookworm ova from wastewater matrices using quantitative PCR

2017 ◽  
Vol 57 ◽  
pp. 231-237 ◽  
Author(s):  
Pradip Gyawali ◽  
Warish Ahmed ◽  
Jatinder P. Sidhu ◽  
Paul Jagals ◽  
Simon Toze
Keyword(s):  
Quantitative Pcr ◽  
Hookworm Ova

scholarly journals Comparison of culture-based, vital stain and PMA-qPCR methods for the quantitative detection of viable hookworm ova

2017 ◽  
Vol 75 (11) ◽  
pp. 2615-2621 ◽  
Author(s):  
P. Gyawali ◽  
J. P. S. Sidhu ◽  
W. Ahmed ◽  
P. Jagals ◽  
S. Toze
Keyword(s):  
Environmental Samples ◽  
Quantitative Measurement ◽  
Quantitative Polymerase Chain Reaction ◽  
Detection Methods ◽  
Quantitative Detection ◽  
Propidium Monoazide ◽  
Chain Reaction ◽  
Vital Stain ◽  
Polymerase Chain ◽  
Hookworm Ova

Accurate quantitative measurement of viable hookworm ova from environmental samples is the key to controlling hookworm re-infections in the endemic regions. In this study, the accuracy of three quantitative detection methods [culture-based, vital stain and propidium monoazide-quantitative polymerase chain reaction (PMA-qPCR)] was evaluated by enumerating 1,000 ± 50 Ancylostoma caninum ova in the laboratory. The culture-based method was able to quantify an average of 397 ± 59 viable hookworm ova. Similarly, vital stain and PMA-qPCR methods quantified 644 ± 87 and 587 ± 91 viable ova, respectively. The numbers of viable ova estimated by the culture-based method were significantly (P < 0.05) lower than vital stain and PMA-qPCR methods. Therefore, both PMA-qPCR and vital stain methods appear to be suitable for the quantitative detection of viable hookworm ova. However, PMA-qPCR would be preferable over the vital stain method in scenarios where ova speciation is needed.

scholarly journals CURRENCY NOTES AND ASSOCIATED RISK OF NEGLECTED TROPICAL DISEASES: STUDY ON THE NIGERIAN NAIRA

2020 ◽  
Vol 7 (12) ◽  
pp. 252-258
Author(s):  
Ukpong Iniodu George ◽  
Joshua Esther Ifenyinwa
Keyword(s):  
Public Health ◽  
Public Education ◽  
Potential Role ◽  
Neglected Tropical Diseases ◽  
Tropical Diseases ◽  
Enterobius Vermicularis ◽  
Oral Pathogens ◽  
And Control ◽  
Hookworm Ova

Currency notes could play a role in the transmission of faeco-oral pathogens. This study aimed at identifying the possible role of the Nigerian Naira in the transmission of some neglected tropical diseases (NTDs) and the implication on their intervention and control in Nigeria. Method: 250 samples of all denominations of mint, dirty and mutilated Naira notes were examined for presence of parasites using the rinse method. Result: 58.4% of notes were contaminated with 161 cysts and ova of a protozoan (45.3%) and helminths (54.7%): Entamoeba histolytica cysts (43.7%), Enterobius vermicularis ova (5.4%), Ascaris lumbricoides ova (34.7%) and Hookworm ova (12.6%). Parasite contamination was independent of currency denomination (X2 =45.4; P=0.05). Mutilated notes harboured more parasites (51.5%) than dirty notes (44.9%). Mint notes had zero contamination. This was statistically significant (X2 =5.6; P<0.05). Polymer notes were more contaminated (51.37%) than paper notes. This study has identified a public health risk and the potential role of the Naira notes in the epidemiology of some NTDs in the study area. Public education on the health implications of the abuse of the Naira, beyond reasons of patriotism is required now. The Central Bank should ensure quick withdrawal of mutilated notes from circulation.

The Effect of Sea-Water on the Development of Hookworm Ova and Larvae (Necator americanus)

1927 ◽  
Vol 13 (4) ◽  
pp. 270 ◽  
Author(s):  
Fred C. Caldwell ◽  
Elfreda L. Caldwell
Keyword(s):  
Sea Water ◽  
Necator Americanus ◽  
Hookworm Ova
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