scholarly journals Antimicrobial Efficacy of Octenidine Hydrochloride, Green Tea, Sodium Hypochlorite and Chlorhexidine Gluconate as Retreatment Endodontic Irrigant Against E. faecalis, Candida & Mixed Culture – In-Vitro Study

2021 ◽  
Vol 10 (32) ◽  
pp. 2629-2633
Author(s):  
Reetika S. Khandelwal ◽  
Shivkumar P. Mantri ◽  
Bonny Paul ◽  
Kavita A. Dube ◽  
Gargi Mishra ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Mixed Culture ◽  
Green Tea ◽  
Sodium Hypochlorite ◽  
Culture Broth ◽  
Chlorhexidine Gluconate ◽  
Antimicrobial Efficacy ◽  
Significant Difference ◽  
Better Than

BACKGROUND Sodium hypochlorite & chlorhexidine are usually employed as endodontic irrigants due to their antimicrobial properties, however, their cytotoxicity & allergenicity issues demand an equally effective but safer irrigant. The purpose of the study was to compare the antimicrobial efficacy of octenidine hydrochloride (OCT), green tea, sodium hypochlorite and chlorhexidine (CHX) at various concentrations against E. faecalis, C. albicans and its mixture. METHODS The study was conducted in Hitkarini Dental College, Jabalpur. Various concentrations of irrigants were prepared. Muller Hinton agar media plates were swabbed with the culture broth of E faecalis ATCC 29212, Candida albicans ATCC 10231 or a mixture of these. Five Whatman paper disks (6 mm), each loaded with experimental concentration of the irrigant, were placed at equal distance on to the plate. The plates were incubated at 370 C for 48 hours. This was done in 6 replicates. The plates were evaluated for clear zone of inhibition measured in mm. RESULTS The diameter readings were analysed using Kruskal Wallis and Mann Whitney U tests. There was a significant difference between the groups against Enterococcus faecalis; candida, & mixed culture (P < 0.01). CONCLUSIONS 5 % Sodium hypochlorite is better than all others whereas 2 % CHX is better than OCT & Green tea. 0.1 % OCT is better than 3 % Green tea. OCT can be used as a contributory irrigant along with NaOCl to achieve antimicrobial advantage. KEY WORDS Antimicrobial Efficacy, Candida Albicans, Chlorhexidine Gluconate, E Faecalis, Green Tea, Octenidine Hydrochloride, Sodium Hypochlorite

scholarly journals An in vitro comparison of Antimicrobial Efficacy of Three Root Canal Irrigants—BioPure MTAD, 2% Chlorhexidine Gluconate and 5.25% Sodium Hypochlorite as a Final Rinse against E. faecalis

2013 ◽  
Vol 14 (5) ◽  
pp. 842-847 ◽  
Author(s):  
MS Rama Rao ◽  
Vinod Agrawal ◽  
Kanupriya Dhingra ◽  
V Rajesh Gopal ◽  
Abhijita Mohapatra
Keyword(s):  
Sodium Hypochlorite ◽  
Root Canal ◽  
Antibacterial Activities ◽  
Chlorhexidine Gluconate ◽  
Antimicrobial Efficacy ◽  
Pulp Tissue ◽  
Root Canal Irrigants ◽  
Significant Difference ◽  
In Vitro Comparison

ABSTRACT Aim This study was conducted to evaluate the antimicrobial activity of 5.25% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX) and BioPure MTAD when used as a final rinse against Enterococcus faecalis. Materials and methods Sixty single-rooted premolars were biomechanically prepared, inoculated with E. faecalis and divided into various groups. These were then irrigated with the test irrigants and tested microbiologically for growth of E. faecalis immediately after irrigation and after 48 hours. Results Statistical analysis showed that there was a significant difference between the antibacterial activities of BioPure MTAD, 2% CHX and 5.25% NaOCl at 5 minutes; however, the antibacterial activities of the three irrigants were comparable after 2 days of irrigation Conclusion The present study concludes that BioPure MTAD is as effective against E. faecalis as 5.25% NaOCl and more effective than 2% CHX. Clinical significance E. faecalis is one of the most resistant intracanal species and a possible cause of root canal failure. Many authors have stressed the importance of using antimicrobial irrigants during chemomechanical preparation to ensure complete disinfection. Therefore, various irrigating solutions have been used during and immediately after root canal preparation to remove debris and necrotic pulp tissue and to eliminate microorganisms that cannot be reached by mechanical instrumentation. How to cite this article Agrawal V, Rao MSR, Dhingra K, Gopal VR, Mohapatra A, Mohapatra A. An in vitro comparison of Antimicrobial Efficacy of Three Root Canal Irrigants—BioPure MTAD, 2% Chlorhexidine Gluconate and 5.25% Sodium Hypochlorite as a Final Rinse against E. faecalis. J Contemp Dent Pract 2013;14(5):842-847.

scholarly journals COMPARATIVE EVALUATION OF ANTIMICROBIAL EFFICACY OF SODIUM HYPOCHLORITE, CHLORHEXIDINE, NEEM AND GREEN TEA AGAINST E. FAECALIS - AN IN VITRO STUDY.

2019 ◽  
Vol 7 (4) ◽  
pp. 449-453
Author(s):  
Vrishali Shrigondekar ◽  
◽  
Jyoti Pawar ◽  
Meenal Gulve ◽  
Roshan Samuel ◽  
...  
Keyword(s):  
Green Tea ◽  
Sodium Hypochlorite ◽  
Comparative Evaluation ◽  
In Vitro Study ◽  
Vitro Study ◽  
Antimicrobial Efficacy

scholarly journals The Effect of Three Different Disinfectant Materials on Polyether Impressions by Spray Method

2012 ◽  
Vol 3 (3) ◽  
pp. 229-233
Author(s):  
Ehsan Ghasemi ◽  
Hamid Badrian ◽  
Nafiseh Hosseini ◽  
Navid Khalighinejad
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Sodium Hypochlorite ◽  
P Value ◽  
Plastic Bags ◽  
Spray Method ◽  
Significant Difference ◽  
Different Types

ABSTRACT Background Dentists, dental equipments and dental laboratories are exposed to different types of pathogenic microorganisms. The aim of this study is to investigate the effect of three different types of disinfectant agents: Sodium hypochlorite 0.525%, Epimax and Deconex, on polyether impressions after 5 and 10 minutes. Materials and methods In this in vitro experimental study, 66 circular samples of polyether impression material of 1 cm diameter and 2 mm thickness were contaminated with Staphylococcus aureus (ATCC29213) Pseudomonas aeruginosa (ATCC27853) and Candida albicans fungus (PTCC5027). Except for control samples, all of them were disinfected with sodium hypochlorite 0.525, Deconex and Epimax by way of spraying. Afterward, they kept in plastic bags with humid rolled cotton for 5 and 10 minutes. In order to isolate bacteria, the samples were immersed in 2% trypsin for 1 hour and then the solution was diluted with normal salin in portion of 1, 1/2, and 1/4. The trypsin suspensions were transferred to culture plates and the number of colonies was counted after 24 and 48 hours for bacteria and after 72 hours for fungus. For data analysis Mann-Whitney statistical test was used (α = 0.05). Results Epimax and sodium hypochlorite thoroughly eliminated Candida albicans as time elapsed from 5 to 10 minutes; however, the other two microorganisms were not eliminated completely. Deconex was completely efficient for all microorganisms when immersion time was enhanced from 5 to 10 minutes. There was a significant difference between efficacy of Deconex-sodium hypochlorite and sodium hypochlorite-Epimax for Pseudomonas aeruginosa in 5-minute immersion (p-value:0/046) Moreover, Deconex and sodium hypochlorite were significantly different for Staphylococcus aureus (p-value:0/046) and Pseudomonas aeruginosa in 10-minute immersion (p < 0.05). Conclusion According to the results of this study, none of the three disinfection materials could eradicate three different kinds of microorganisms in 5 minutes, but in 10 minutes, Deconex could completely eradicate all microorganisms compared to other disinfection agents, which is a good indicator for high efficacy of this agent in disinfecting polyether impressions. How to cite this article Ghasemi E, Badrian H, Hosseini N, Khalighinejad N. The Effect of Three Different Disinfectant Materials on Polyether Impressions by Spray Method. World J Dent 2012;3(3):229-233.

Antimicrobial Efficacy of a Novel Root Canal Irrigant Made from Coconut and Bromelain Extract against Biofilm Forming Pathogens– An In vitro Evaluation

2021 ◽  
pp. 5222-5226
Author(s):  
Jerry Jose ◽  
Ajitha Palanivelu
Keyword(s):  
Candida Albicans ◽  
Enterococcus Faecalis ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Antimicrobial Efficacy ◽  
Diffusion Method ◽  
Measured Quantity ◽  
Zone Of Inhibition ◽  
Root Canal Irrigant

Objective: Sodium hypochlorite is shown to be a gold standard intracanal irrigant which has shown to have adequate antimicrobial properties and tissue dissolving properties. The toxicity of these irrigants are seen to be high towards various cells. Natural irrigants provide an alternative to show adequate antimicrobial activity and lesser cytotoxicity potential. The current study proposes a novel intracanal irrigant made from coconut and bromelain extract. Material and Methods: 10grams of sodium hydroxide was dissolved in deionized water followed by which 60ml of coconut oil was added to the warm water bath (40C). The final product was left to dry and crushed manually to powder. This powder was then taken in measured quantity to make a final product containing 20% of coconut and 4% of bromelain. The obtained irrigant was then evaluated against Enterococcus faecalis using Agar well diffusion method. Results: For the natural irrigant, the maximum zone of inhibition was seen highest at 22mm against Enterococcus faecalis and 25mm against Candida Albicans. For 3% sodium hypochlorite when compared against Enterococcus faecalis and Candida Albicans showed a maximum zone of inhibition at 33mm against Enterococcus faecalis and 35mm against Candida Albicans. Conclusion: The study shows that the novel irrigant derived from coconut and bromelain extract has a good antimicrobial efficacy against Enterococcus faecalis and Candida albicans. It can be concluded that it has a potential application as a root canal irrigant for root canal disinfection.

scholarly journals Comparison of cytotoxicity of various concentrations origanum extract solution with 2% chlorhexidine gluconate and 5.25% sodium hypochlorite

2015 ◽  
Vol 09 (01) ◽  
pp. 006-010 ◽  
Author(s):  
Evren Ok ◽  
Necdet Adanir ◽  
Sema Hakki
Keyword(s):  
Sodium Hypochlorite ◽  
Cytotoxic Effect ◽  
Statistical Significance ◽  
In Vitro Study ◽  
Solution Concentration ◽  
Chlorhexidine Gluconate ◽  
Extract Solution ◽  
Root Canal Irrigation ◽  
Significant Difference

ABSTRACT Objective: The purpose of this in vitro study was to compare the cytotoxicity of 0.5–4.5 origanum extract solution (OES), 2% chlorhexidine gluconate (CHX) and 5.25% sodium hypochlorite (NaOCl) with WST-1 test on human periodontal ligament (hPDL) fibroblasts. Materials and Methods: About 0.5–4.5% OES, 2% CHX and 5.25% NaOCl solutions cytotoxicity was evaluated with cell culture test using PDL fibroblasts. Viability of hPDL cells was evaluated with WST-1 (Cell Proliferation Reagent WST-1 Roche) test at 1, 24 and 72nd h. hPDL cells were plated at 20 × 103 cells per well in 96-well plates. Absorbance values were read in optical density 480 nm by ELISA plate reader spectrophotometer. The statistical differences between various groups were evaluated using one-way ANOVA, post-hoc Duncan's Multiple Range test using SAS software. Statistically, a significant difference was considered at P < 0.001. Results: According to the 1-h cytotoxicity results, 0.5% OES showed the least cytotoxic effect in test groups. There were not found any statistical significance between 1% OES and 2% CHX. About 5.25% NaOCl showed more cytotoxic effect than 1% OES and 2% CHX. In 24 and 72 h, different concentrations of OES, 5.25% NaOCl, 2% CHX solutions showed similar cytotoxic effect. Conclusions: Based on these results, 1% OES and 2% CHX showed similar results and less cytotoxic effect than 5.25% NaOCl. It could be considered as a favorable solution concentration when OES was used as root canal irrigation solution.

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