scholarly journals ANTHOCYANINS OF BASIL LEAVES: DETERMINATION AND PREPARATION OF DRIED ENCAPSULATED FORMS

2017 ◽  
pp. 129-135 ◽  
Author(s):  
Виктор (Viktor) Иванович (Ivanovich) Дейнека (Deineka) ◽  
Ярослава (Iaroslava) Юрьевна (Iur'evna) Кульченко (Kul'chenko) ◽  
Ирина (Irina) Петровна (Petrovna) Блинова (Blinova) ◽  
Андрей (Andrei) Николаевич (Nikolaevich) Чулков (Chulkov) ◽  
Людмила (Liudmila) Александровна (Aleksandrovna) Дейнека (Deineka)
Keyword(s):  
Mass Spectra ◽  
High Stability ◽  
Reversed Phase ◽  
Ocimum Basilicum ◽  
Mass Spectrometric ◽  
Reversed Phase Hplc ◽  
Cinnamic Acids ◽  
Spectra Analysis ◽  
Red Color ◽  
High Level

Anthocyanins composition of five purple leaves cultivars of Ocimum basilicum L. was investigated by reversed-phase HPLC with mass-spectrometric detection in the conditions of ions partial fragmentation as well as preparation of dried differently colored forms of anthocyanins encapsulated into maltodextrin matrix. Mass-spectra analysis revealed that according to anthocyanin set basil cultivars under investigation may be divided onto two groups with common feature being high level of acylation with (mainly) p-coumaric, ferulic and malonic acids of the same base – cyanidin-3-dihexoside-5-hexoside. The presence of acylation with substituted cinnamic acids permits to obtain solutions not only with red color (the property of the flavylium form) but also with blue shades coloration due to quinoid and negatively charged quinoid forms. All forms exept of flavylium are not stable in solution but stable enough to prepare dried encapsulated forms by lyophilization. And thou the loss of anthocyanins at drying is not negligible the final product is characterized with high stability at storage in refrigerator.

Characterization of a goat whey peptic hydrolysate produced by an ultrafiltration membrane enzymic reactor

2000 ◽  
Vol 67 (4) ◽  
pp. 551-559 ◽  
Author(s):  
STÉPHANIE BORDENAVE ◽  
FRÉDÉRIC SANNIER ◽  
GUY RICART ◽  
JEAN-MARIE PIOT
Keyword(s):  
Reversed Phase ◽  
Ultrafiltration Membrane ◽  
Reversed Phase Hplc ◽  
Spectra Analysis ◽  
Membrane Peptides ◽  
Derivative Spectra ◽  
Wide Range ◽  
Molecular Masses ◽  
Β Lactoglobulin

Goat whey was hydrolysed by pepsin in an ultrafiltration membrane enzymic reactor coupled with a 30 kDa mineral membrane. Peptides collected in the permeate were resolved using reversed-phase HPLC. Their sequences were determined by amino acid analysis, second order derivative spectra analysis and mass spectrometry. Owing to the resistance of β-lactoglobulin (β-lg) towards pepsin, the majority of peptides identified were derived from α-lactalbumin (α-la). Pepsin showed a broad specificity of hydrolysis sites and generated a wide range of products from dipeptides to very large peptides containing disulphide bridges. The molecular masses of peptides resulting from α-la degradation were between 150 and 6900 Da: 36% were < 600 Da, 24% were 600–2000 Da and 40% were > 2000 Da.

scholarly journals DETERMINATION OF ANTHOCYANINS OF FRUITS OF SOME PLANTS OF THE GENUS RIBES BY REVERSED-PHASE HPLC AND HYDROPHILIC INTERACTION CHROMATOGRAPHY (HILIC)

2020 ◽  
pp. 81-88
Author(s):  
Viktor Ivanovich Deineka ◽  
Elena Yur'yevna Oleinits ◽  
Aleksandr Alekseyevich Pavlov ◽  
Andrey Yur'yevich Mikheev ◽  
Ol'ga Vladimirovna Shelepova ◽  
...  
Keyword(s):  
Stationary Phases ◽  
Gradient Elution ◽  
Botanical Garden ◽  
Reversed Phase ◽  
Hydrophilic Interaction Chromatography ◽  
Coumaric Acid ◽  
Reversed Phase Hplc ◽  
Hydrophilic Interaction ◽  
Cinnamic Acids

The anthocyanin composition of some species of gooseberries with colored fruits was studied in comparison with anthocyanins of currant fruits from the collection of the Tsitsin Main Botanical garden. In fruits of currants Ribes nigrum var. sibiricum E. Wolf, R. nigrum L., R. americanum Mill., as well as in dark-colored fruits R. aureum Pursh., 3-glucosides and 3-rutinosides of cyanidin and delphinidin were found as major components in species-dependent ratios. It was found that in the fruits of gooseberry species Ribes oxyacanthoides L., Ribes missouriensis (Nutt.) Coville & Britton, Ribes rusticum Jancz. and Ribes cynosbati (L.) Mill. and in the fruits of one of the currants (R. alpinum L.) there is practically no biosynthesis of delphinidin derivatives, and 3-glucoside and 3-rutinoside of cyanidin are detected. Meanwhile, the biosynthesis of acylated by substituted cinnamic acids (mainly – p-coumaric acid) anthocyanins were found in the fruits of Ribes oxyacanthoides and Ribes cynosbati (about 10%), Ribes rusticum (about 20%). The inheritance of characteristic for the fruit of some species of gooseberry biosynthesis of acylated mainly p-coumaric acid, was installed in the fruits of jostaberry - Ribes x nidigrolaria Rud. Bauer & A. Bauer (about 10%). The species composition of anthocyanins was established using traditional reversed-phase HPLC in gradient elution mode using diode-array and mass spectrometric detection options. In addition, a method for the separation of anthocyanins of the same objects in the isocratic mode under hydrophilic interaction chromatography on diol stationary phases, allowing the use of simpler equipment. The variants of sample preparation of extract samples for the subsequent HPLC determination of anthocyanins by two different methods to avoid the appearance of artifacts are proposed.

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