In vitro caries formation in primary tooth enamel

2006 ◽  
Vol 137 (5) ◽  
pp. 638-644 ◽  
Author(s):  
Gary H. Westerman ◽  
M. John Hicks ◽  
Catherine M. Flaitz ◽  
G. Lynn Powell
Keyword(s):  
Tooth Enamel ◽  
Primary Tooth

scholarly journals Effect of Nd:YAG laser combined with fluoride on the prevention of primary tooth enamel demineralization

2012 ◽  
Vol 23 (2) ◽  
pp. 104-109 ◽  
Author(s):  
Danielle Torres Azevedo ◽  
Juliana Jendiroba Faraoni-Romano ◽  
Juliana dos Reis Derceli ◽  
Regina Guenka Palma-Dibb
Keyword(s):  
Nd:Yag Laser ◽  
Tooth Enamel ◽  
Fluoride Varnish ◽  
Permanent Teeth ◽  
Primary Tooth ◽  
Preventive Action ◽  
Contact Mode ◽  
Enamel Demineralization ◽  
Nail Polish

Most studies dealing with the caries preventive action of Nd:YAG laser have been done in permanent teeth and studies on primary teeth are still lacking. The aim of this study was to evaluate in vitro the effect of Nd:YAG laser combined or not with fluoride sources on the acid resistance of primary tooth enamel after artificial caries induction by assessing longitudinal microhardness and demineralization depth. Sixty enamel blocks obtained from the buccal/lingual surface of exfoliated human primary molars were coated with nail polish/wax, leaving only a 9 mm² area exposed on the outer enamel surface, and randomly assigned to 6 groups (n=10) according to the type of treatment: C-control (no treatment); APF: 1.23% acidulated phosphate fluoride gel; FV: 5% fluoride varnish; L: Nd:YAG laser 0.5 W/10 Hz in contact mode; APFL: fluoride gel + laser; FVL: fluoride varnish + laser. After treatment, the specimens were subjected to a des-remineralization cycle for induction of artificial caries lesions. Longitudinal microhardness data (%LMC) were analyzed by the Kruskal-Wallis test and demineralization depth data were analyzed by oneway ANOVA and Fisher’s LSD test (á=0.05). APFL and APF groups presented the lowest percentage of microhardness change (p<0.05). Demineralization depth was smaller in all treated groups compared with the untreated control. In conclusion, Nd:YAG laser combined or not with fluoride gel/varnish was not more effective than fluoride alone to prevent enamel demineralization within the experimental period.

In vitro occlusal wear of restorative materials on primary teeth

2005 ◽  
Vol 29 (3) ◽  
pp. 221-224 ◽  
Author(s):  
P. Villalta ◽  
C.R. Rodrigues
Keyword(s):  
Tooth Enamel ◽  
Pediatric Dentistry ◽  
Permanent Tooth ◽  
Primary Tooth ◽  
In Vitro Test ◽  
Test Protocol ◽  
Occlusal Wear ◽  
Restorative Materials ◽  
Materials Used

Since primary tooth enamel wears more rapidly than permanent tooth enamel, it is important to study the mechanical wear patterns of restorative materials used in the primary dentition. It is important that an in vitro evaluation of wear resistance of different restorative materials is done in order to establish a valid in vitro test protocol for use in pediatric dentistry.

scholarly journals Effect of conventional and sugar free pediatric syrup formulations on primary tooth enamel hardness: An in vitro study

2015 ◽  
Vol 33 (4) ◽  
pp. 331 ◽  
Author(s):  
GauraoVasant Mali ◽  
ArunSuresh Dodamani ◽  
GundabakthaNagappa Karibasappa ◽  
PrashanthVishwakarma Kumar ◽  
VardhamanMulchand Jain
Keyword(s):  
Tooth Enamel ◽  
In Vitro Study ◽  
Vitro Study ◽  
Primary Tooth

Assessing the Effect of Low Calorie Soda Beverages on Primary Tooth Enamel: An In Vitro Study

2019 ◽  
Vol 43 (3) ◽  
pp. 190-195 ◽  
Author(s):  
Alexandra Korte ◽  
Matina V Angelopoulou ◽  
Georgios Maroulakos
Keyword(s):  
Surface Roughness ◽  
Tooth Enamel ◽  
In Vitro Study ◽  
Primary Tooth ◽  
Dietary Advice ◽  
Low Calorie ◽  
Treatment Groups ◽  
Dental Office ◽  
Before And After

Objective: The purpose of this study was to evaluate the effect of low calorie soda beverages on the enamel of primary teeth. Study Design: Fifty enamel slabs were prepared from twenty primary extracted teeth and were equally divided into five groups: a) 0.9% NaCl (Control), b) Coca-Cola Classic (Sucrose), c) Diet Coke (Aspartame), d) Zevia Cola (Erythritol), e) Coca-Cola Life (Stevia). Each specimen was exposed to the beverage for a total of sixty minutes. Enamel surface roughness was measured before and after the exposures using a LEXT OLS4000 3D Laser Measuring Microscope. Results: All tested sodas resulted to a statistically significant change on the surface roughness of the enamel (p =.000). However, this effect did not differ significantly between the different treatment groups (p =.103). Conclusions: Both regular soda and low calorie soda containing different commercial sweeteners appear to have an effect on the surface morphology of primary tooth enamel. Thus, it is important to discourage the intake of any type of soda as part of the dietary advice provided in the dental office.

scholarly journals Evaluation of Change in Microhardness by Application of MI Varnish on Primary Tooth Enamel, Affected by Use of Frequently Prescribed Paediatric Syrups: An In Vitro Study

Cureus ◽  
2020 ◽  
Author(s):  
Ankita Maurya ◽  
N.D. Shashikiran ◽  
Namrata Gaonkar ◽  
Sachin Gugawad ◽  
Swapnil Taur ◽  
...  
Keyword(s):  
Tooth Enamel ◽  
In Vitro Study ◽  
Vitro Study ◽  
Primary Tooth

scholarly journals Bond Durability of Er:YAG Laser-Prepared Primary Tooth Enamel

2013 ◽  
Vol 24 (4) ◽  
pp. 330-334 ◽  
Author(s):  
Maria Cristina Borsatto ◽  
Mayara Garcia Martinelli ◽  
Marta Maria Martins Giamatei Contente ◽  
Talitha de Siqueira Mellara ◽  
Jesus Djalma Pecora ◽  
...  
Keyword(s):  
Shear Bond Strength ◽  
Composite Resin ◽  
Er:Yag Laser ◽  
Tooth Enamel ◽  
Water Storage ◽  
Adhesive System ◽  
Primary Tooth ◽  
Cavity Preparation ◽  
Etch And Rinse

This study evaluated in vitro the influence of thermocycling and water storage (WS) on the shear bond strength (SBS) of composite resin in cavities prepared in primary tooth enamel with conventional bur or Er:YAG laser. The test surfaces were obtained from 48 primary molars and randomly assigned to 2 groups (n=24), according to cavity preparation: A: bur-preparation and B: Er:YAG laser irradiation. The specimens were restored with an etch-and-rinse adhesive system and composite resin. Each group was divided into 4 subgroups (n=6) according to WS duration and number of thermal cycles (TCs): I: 24 h WS/no thermocycling; II: 7 days WS/500 TCs; III: 1 month WS/2,000 TCs; IV: 6 months WS/12,000 TCs. The specimens were tested to failure in shear strength at a crosshead speed of 0.5 mm/min. Data were analyzed statistically by two-way ANOVA and Tukey's test. SBS means (S.D.) in MPa were: AI: 17.45 (2.03), AII:16.38 (1.49), AIII: 6.88 (0.66), AIV: 7.77 (1.53), BI: 12.32 (0.99), BII: 15.37 (2.24), BIII: 15.05 (2.01) and BIV-5.51 (1.01). WS duration and number of TCs influenced significantly the SBS values only for BIV (p<0.05). AI presented the highest SBS value, which was statistically similar to those of AII, BII and BIII. In conclusion, the adhesion of an etch-and-rinse adhesive to Er:YAG laser-irradiated primary tooth enamel was affected by the methods used to simulate degradation of the adhesive interface only when 6 months WS/12,000 TCs were employed.

scholarly journals In Vitro Erosive Effect of Pediatric Medicines on Deciduous Tooth Enamel

2014 ◽  
Vol 25 (1) ◽  
pp. 22-27 ◽  
Author(s):  
Camila Scatena ◽  
Daniel Galafassi ◽  
Jaciara Miranda Gomes-Silva ◽  
Maria Cristina Borsatto ◽  
Mônica Campos Serra
Keyword(s):  
Exposure Time ◽  
Ferrous Sulfate ◽  
Tooth Enamel ◽  
Artificial Saliva ◽  
Titratable Acidity ◽  
Primary Tooth ◽  
Control Group ◽  
Salbutamol Sulfate ◽  
Sem Images

This study evaluated, in vitro, the erosive potential of pediatric liquid medicines in primary tooth enamel, depending on the exposure time. Sixty deciduous incisors were randomly assigned to 4 groups (n=15), according to the immersion solutions: guaifenesin; ferrous sulfate; salbutamol sulfate and artificial saliva. The immersion cycles in the medicines were undertaken under a 1-min agitation, which wasperformed three times daily, during 28 days. Surface microhardness was measured at 7,14, 21 and 28 days. The titratable acidity and buffering capacity of the immersion media were determined. Data were analyzed by Analysis of Variance and Tukey's test (α=0.05). Salbutamol sulfate caused a gradual loss in enamel microhardness deciduous, observed at all times (p<0.005). Exposure to guaifenesin or ferrous sulfate resulted in significant decrease of enamel microhardness only after 28 days (p<0.005). In the control group (artificial saliva), microhardness did not changed (p>0.005) at any of the studied times. Scanning Electron Microscopy (SEM) images revealed that after 28 days the surfaces clearly exhibited structural loss, which was unlike those immersed in artificial saliva. Erosion of deciduous enamel was dependent on the type of medicine and exposure time.

scholarly journals Preventing and arresting primary tooth enamel lesions using self-assembling peptide P11-4 in vitro

2021 ◽  
Author(s):  
Nour Wahba ◽  
Falk Schwendicke ◽  
Mohamed Amr Kamel ◽  
Gehan Allam ◽  
Sebastian Paris ◽  
...  
Keyword(s):  
Tooth Enamel ◽  
Minimal Invasive ◽  
Primary Tooth ◽  
Treatment Needs ◽  
Invasive Treatment ◽  
Self Assembling ◽  
Ph Cycling ◽  
Casein Phosphopeptide ◽  
Negative Controls

Abstract Background: Self-assembling peptides (SAP) may prevent and/or arrest caries lesions. The aim of the study was to evaluate SAP for caries prevention and arrest in primary tooth enamel in vitro. Methods: 215 extracted primary teeth were used. In the prevention experiment, self-assembling peptide for prevention (SAPP), fluoride varnish/mouthwash (FV/FMW), casein-phosphopeptide amorphous-calcium phosphate (CPP-ACP), nanohydroxy-apatite (nHA) were applied. Samples were subjected to a demineralizing pH-cycling for 14-days. In the arrest experiment, 60 samples were pre-demineralized and induced lesions treated using self-assembling peptide for repair (SAPR), FV, CPP-ACP plus fluoride and resin infiltration (RI), and submitted to pH-cycling. Thirty-five samples were used as negative controls (NC). Mineral loss and its differences (Δ/ΔΔZ) were determined using transversal microradiography. Results: FV (ΔZ median: -46 [interquartile range: 189] vol%×µm) and FMW (-28 [129] vol%×µm) prevented caries significantly more effective than all other groups (p<0.05), which did not show significant preventive effects compared with NC (ΔZ=1446 [378] vol%×µm). RI (ΔΔZ=1808 [2193] vol%×µm) and FV (ΔΔZ=1494 [4274] vol%×µm) arrested lesions compared with NC (ΔΔZ=5605 [1371] vol%×µm; p<0.05), while SAPR and CPP-ACPF did not show such arrest. Conclusions: FV and FMW showed the largest caries-preventive effect, while RI and FV arrested lesion progression in primary tooth enamel in vitro. Clinical significance: Preventing and arresting caries lesion allows decreasing invasive treatment needs and patient’s morbidity and fosters the application of minimal invasive dentistry approach.

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