scholarly journals Metagenomic approach of associated fungi with Megaplatypus mutatus (Coleoptera: Platypodinae)

Silva Fennica ◽  
2018 ◽  
Vol 52 (3) ◽  
Author(s):  
Esteban Ceriani-Nakamurakare ◽  
Sergio Ramos ◽  
Carolina Robles ◽  
María Novas ◽  
María D´Jonsiles ◽  
...  
Keyword(s):  
Metagenomic Approach ◽  
Megaplatypus Mutatus

scholarly journals Characterization of efficient xylanases from industrial-scale pulp and paper wastewater treatment microbiota

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jia Wang ◽  
Jiawei Liang ◽  
Yonghong Li ◽  
Lingmin Tian ◽  
Yongjun Wei
Keyword(s):  
Wastewater Treatment ◽  
Xylanase Activity ◽  
Pulp And Paper ◽  
Optimal Conditions ◽  
Paper Wastewater ◽  
Termite Hindgut ◽  
Uncultured Microorganisms ◽  
Metagenomic Approach ◽  
Gh10 Family

AbstractXylanases are widely used enzymes in the food, textile, and paper industries. Most efficient xylanases have been identified from lignocellulose-degrading microbiota, such as the microbiota of the cow rumen and the termite hindgut. Xylanase genes from efficient pulp and paper wastewater treatment (PPWT) microbiota have been previously recovered by metagenomics, assigning most of the xylanase genes to the GH10 family. In this study, a total of 40 GH10 family xylanase genes derived from a certain PPWT microbiota were cloned and expressed in Escherichia coli BL21 (DE3). Among these xylanase genes, 14 showed xylanase activity on beechwood substrate. Two of these, PW-xyl9 and PW-xyl37, showed high activities, and were purified to evaluate their xylanase properties. Values of optimal pH and temperature for PW-xyl9 were pH 7 and 60 ℃, respectively, while those for PW-xyl37 were pH 7 and 55 ℃, respectively; their specific xylanase activities under optimal conditions were 470.1 U/mg protein and 113.7 U/mg protein, respectively. Furthermore, the Km values of PW-xyl9 and PW-xyl37 were determined as 8.02 and 18.8 g/L, respectively. The characterization of these two xylanases paves the way for potential application in future pulp and paper production and other industries, indicating that PPWT microbiota has been an undiscovered reservoir of efficient lignocellulase genes. This study demonstrates that a metagenomic approach has the potential to screen efficient xylanases of uncultured microorganisms from lignocellulose-degrading microbiota. In a similar way, other efficient lignocellulase genes might be identified from PPWT treatment microbiota in the future.

scholarly journals Evolutionarily recent dual obligatory symbiosis among adelgids indicates a transition between fungus- and insect-associated lifestyles

2021 ◽  
Author(s):  
Gitta Szabó ◽  
Frederik Schulz ◽  
Alejandro Manzano-Marín ◽  
Elena Rebecca Toenshoff ◽  
Matthias Horn
Keyword(s):  
Defense Mechanisms ◽  
Amino Acid Production ◽  
Evolutionary Transition ◽  
Evolutionary Time ◽  
Evolutionary Trajectories ◽  
Host Infection ◽  
Phylogenomic Analyses ◽  
Time Specific ◽  
High Level ◽  
Metagenomic Approach

AbstractAdelgids (Insecta: Hemiptera: Adelgidae) form a small group of insects but harbor a surprisingly diverse set of bacteriocyte-associated endosymbionts, which suggest multiple replacement and acquisition of symbionts over evolutionary time. Specific pairs of symbionts have been associated with adelgid lineages specialized on different secondary host conifers. Using a metagenomic approach, we investigated the symbiosis of the Adelges laricis/Adelgestardus species complex containing betaproteobacterial (“Candidatus Vallotia tarda”) and gammaproteobacterial (“Candidatus Profftia tarda”) symbionts. Genomic characteristics and metabolic pathway reconstructions revealed that Vallotia and Profftia are evolutionary young endosymbionts, which complement each other’s role in essential amino acid production. Phylogenomic analyses and a high level of genomic synteny indicate an origin of the betaproteobacterial symbiont from endosymbionts of Rhizopus fungi. This evolutionary transition was accompanied with substantial loss of functions related to transcription regulation, secondary metabolite production, bacterial defense mechanisms, host infection, and manipulation. The transition from fungus to insect endosymbionts extends our current framework about evolutionary trajectories of host-associated microbes.

Metagenomic approach to the study of halophages: the environmental halophage 1

2007 ◽  
Vol 9 (7) ◽  
pp. 1711-1723 ◽  
Author(s):  
Fernando Santos ◽  
Anke Meyerdierks ◽  
Arantxa Peña ◽  
Ramon Rosselló-Mora ◽  
Rudolf Amann ◽  
...  
Keyword(s):  
Metagenomic Approach

Identification of bacteria associated with underground parts of Crocus sativus by 16S rRNA gene targeted metagenomic approach

2014 ◽  
Vol 30 (10) ◽  
pp. 2701-2709 ◽  
Author(s):  
Sheetal Ambardar ◽  
Naseer Sangwan ◽  
A. Manjula ◽  
J. Rajendhran ◽  
P. Gunasekaran ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Crocus Sativus ◽  
Rrna Gene ◽  
Identification Of Bacteria ◽  
Metagenomic Approach

scholarly journals Soil Bacterial Community Shifts after Chitin Enrichment: An Integrative Metagenomic Approach

PLoS ONE ◽  
2013 ◽  
Vol 8 (11) ◽  
pp. e79699 ◽  
Author(s):  
Samuel Jacquiod ◽  
Laure Franqueville ◽  
Sébastien Cécillon ◽  
Timothy M. Vogel ◽  
Pascal Simonet
Keyword(s):  
Bacterial Community ◽  
Soil Bacterial Community ◽  
Community Shifts ◽  
Soil Bacterial ◽  
Metagenomic Approach

Identification of a Novel Gene Through the Metagenomic Approach to Degrade the Targeted Pollutant

2019 ◽  
pp. 204-227
Author(s):  
Suriya Jayaraman ◽  
Suganya Thangaiyan ◽  
Kannan Mani ◽  
Kayalvizhi Nagarajan ◽  
Krishnan Muthukalingan
Keyword(s):  
Novel Gene ◽  
Metagenomic Approach
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