scholarly journals Intracellular localizations of the Dead End protein in Xenopus primordial germ cells

2014 ◽  
Vol 58 (10-11-12) ◽  
pp. 793-798 ◽  
Author(s):  
Ayaka Taguchi ◽  
Kenji Watanabe ◽  
Hidefumi Orii
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Dead End ◽  
The Dead

scholarly journals Dnd1 Knockout in Sturgeons By CRISPR/Cas9 Generates Germ Cell Free Host for Surrogate Production

Animals ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 174 ◽  
Author(s):  
Abdul Rasheed Baloch ◽  
Roman Franěk ◽  
Tomáš Tichopád ◽  
Michaela Fučíková ◽  
Marek Rodina ◽  
...  
Keyword(s):  
Germ Cells ◽  
Small Body ◽  
Primordial Germ Cells ◽  
Natural Habitats ◽  
Embryo Survival ◽  
Acipenser Ruthenus ◽  
Dead End ◽  
Living Fossils ◽  
Sturgeon Species ◽  
Surrogate Production

Sturgeons also known as living fossils are facing threats to their survival due to overfishing and interference in natural habitats. Sterlet (Acipenser ruthenus) due to its rapid reproductive cycle and small body size can be used as a sterile host for surrogate production for late maturing and large sturgeon species. Dead end protein (dnd1) is essential for migration of Primordial Germ Cells (PGCs), the origin of all germ cells in developing embryos. Knockout or knockdown of dnd1 can be done in order to mismigrate PGCs. Previously we have used MO and UV for the aforementioned purpose, and in our present study we have used CRISPR/Cas9 technology to knockout dnd1. No or a smaller number of PGCs were detected in crispants, and we also observed malformations in some CRISPR/Cas9 injected embryos. Furthermore, we compared three established methods to achieve sterility in sterlet, and we found higher embryo survival and hatching rates in CRISPR/Cas9, UV and MO, respectively.

scholarly journals Chicken Dead End Homologue Protein is a Nucleoprotein of Germ Cells Including Primordial Germ Cells

2009 ◽  
Vol 55 (2) ◽  
pp. 214-218 ◽  
Author(s):  
Shinya ARAMAKI ◽  
Kaiyu KUBOTA ◽  
Tomoki SOH ◽  
Nobuhiko YAMAUCHI ◽  
Masa-aki HATTORI
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Dead End

MOLECULAR CLONING OF CHICKEN DEAD END HOMOLOGUE (CDH) AND IDENTIFICATION OF PRIMORDIAL GERM CELLS USING CDH GENE

2007 ◽  
Vol 77 (Suppl_1) ◽  
pp. 87-87
Author(s):  
Shinya Aramaki ◽  
Fuminori Sato ◽  
Takako Kato ◽  
Yukio Kato ◽  
Masa-aki Hattori
Keyword(s):  
Molecular Cloning ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Dead End

Molecular cloning and expression of dead end homologue in chicken primordial germ cells

2007 ◽  
Vol 330 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Shinya Aramaki ◽  
Fuminori Sato ◽  
Takako Kato ◽  
Tomoki Soh ◽  
Yukio Kato ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Germ Cells ◽  
Primordial Germ Cells ◽  
Cloning And Expression ◽  
Dead End

Hexachlorobenzene toxicity in the rat ovary: II. Ultrastructure induced by medium (10 mg/kg) dose exposure

1992 ◽  
Vol 50 (1) ◽  
pp. 668-669
Author(s):  
Amreek Singh ◽  
Warren G. Foster ◽  
Anna Dykeman ◽  
David C. Villeneuve
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells ◽  
Surface Epithelium ◽  
Morphological Parameters ◽  
Comprehensive Investigation ◽  
Ovary Surface Epithelium ◽  
Rat Ovary ◽  
High Doses

Hexachlorobenzene (HCB) is a known toxicant that is found in the environment as a by-product during manufacture of certain pesticides. This chlorinated chemical has been isolated from many tissues including ovary. When administered in high doses, HCB causes degeneration of primordial germ cells and ovary surface epithelium in sub-human primates. A purpose of this experiment was to determine a no-effect dose of the chemical on the rat ovary. The study is part of a comprehensive investigation on the effects of the compound on the biochemical, hematological, and morphological parameters in the monkey and rat.

Transfusion of Chick Primordial Germ Cells into Quail Embryos and their Settlement in Gonads

1998 ◽  
Vol 69 (10) ◽  
pp. 911-915 ◽  
Author(s):  
Tamao ONO ◽  
Ryohei YOKOI ◽  
Seishi MAEDA ◽  
Takao NISHIDA ◽  
Hirohiko AOYAMA
Keyword(s):  
Germ Cells ◽  
Primordial Germ Cells
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