scholarly journals Signaling pathways dictating pluripotency in embryonic stem cells

2013 ◽  
Vol 57 (9-10) ◽  
pp. 667-675 ◽  
Author(s):  
Debasree Dutta
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Signaling Pathways ◽  
Embryonic Stem

scholarly journals Partial promoter substitutions generating transcriptional sentinels of diverse signaling pathways in embryonic stem cells and mice

2012 ◽  
Vol 5 (6) ◽  
pp. 956-966 ◽  
Author(s):  
P. Serup ◽  
C. Gustavsen ◽  
T. Klein ◽  
L. A. Potter ◽  
R. Lin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Signaling Pathways ◽  
Embryonic Stem

scholarly journals Integration of External Signaling Pathways with the Core Transcriptional Network in Embryonic Stem Cells

Cell ◽  
2008 ◽  
Vol 133 (6) ◽  
pp. 1106-1117 ◽  
Author(s):  
Xi Chen ◽  
Han Xu ◽  
Ping Yuan ◽  
Fang Fang ◽  
Mikael Huss ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Signaling Pathways ◽  
Embryonic Stem ◽  
Transcriptional Network ◽  
The Core

Pre-differentiated Embryonic Stem Cells Promote Neuronal Regeneration by Cross-coupling of BDNF and IL-6 Signaling Pathways in the Host Tissue.

2009 ◽  
pp. 090330061141047
Author(s):  
Margarita Glazova ◽  
Elena S. Pak ◽  
Justin Moretto ◽  
Sarah Hollis ◽  
Kori L. Brewer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Signaling Pathways ◽  
Embryonic Stem ◽  
Cross Coupling ◽  
Host Tissue ◽  
Neuronal Regeneration

CDK11 safeguards the identity of human embryonic stem cells via fine‐tuning signaling pathways

2019 ◽  
Vol 235 (5) ◽  
pp. 4279-4290
Author(s):  
Jianyi Ding ◽  
Zhuoqing Fang ◽  
Xinyuan Liu ◽  
Zhexin Zhu ◽  
Chunsheng Wen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Signaling Pathways ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Fine Tuning

scholarly journals Expression profiles of protein tyrosine kinase genes in human embryonic stem cells

Reproduction ◽  
2008 ◽  
Vol 136 (4) ◽  
pp. 423-432 ◽  
Author(s):  
Mi-Young Son ◽  
Janghwan Kim ◽  
Hyo-Won Han ◽  
Sun-Mi Woo ◽  
Yee Sook Cho ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Signaling Pathways ◽  
Human Embryonic Stem Cells ◽  
Tyrosine Kinases ◽  
Expression Profiles ◽  
Embryonic Stem ◽  
Degenerate Primers ◽  
Protein Tyrosine ◽  
Hesc Lines

Complex signaling pathways operate in human embryonic stem cells (hESCs) and are coordinated to maintain self-renewal and stem cell characteristics in them. Protein tyrosine kinases (PTKs) participate in diverse signaling pathways in various types of cells. Because of their functions as key molecules in various cellular processes, PTKs are anticipated to have important roles also in hESCs. In this study, we investigated the roles of PTKs in undifferentiated and differentiated hESCs. To establish comprehensive PTK expression profiles in hESCs, we performed reverse transcriptase PCR using degenerate primers according to the conserved catalytic PTK motifs in both undifferentiated and differentiated hESCs. Here, we identified 42 different kinases in two hESC lines, including 5 non-receptor tyrosine kinases (RTKs), 24 RTKs, and 13 dual and other kinases, and compared the protein kinase expression profiles of hESCs and retinoic acid-treated hESCs. Significantly, up- and downregulated kinases in undifferentiated hESCs were confirmed by real-time PCR and western blotting. MAP3K3, ERBB2, FGFR4, and EPHB2 were predominantly upregulated, while CSF1R, TYRO3, SRC, and GSK3A were consistently downregulated in two hESC lines. Western blot analysis showed that the transcriptional levels of these kinases were consistent with the translational levels. The obstruction of upregulated kinases’ activities using specific inhibitors disturbed the undifferentiated status and induced the differentiation of hESCs. Our results support the dynamic expression of PTKs during hESC maintenance and suggest that specific PTKs that are consistently up- and downregulated play important roles in the maintenance of stemness and the direction of differentiation of hESCs.

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