scholarly journals Role of mitochondrial DNA replication during differentiation of reprogrammed stem cells

2010 ◽  
Vol 54 (11-12) ◽  
pp. 1659-1670 ◽  
Author(s):  
Richard D.W. Kelly ◽  
Justin C. St. John
Keyword(s):  
Stem Cells ◽  
Mitochondrial Dna ◽  
Dna Replication ◽  
Mitochondrial Dna Replication

scholarly journals Primer retention owing to the absence of RNase H1 is catastrophic for mitochondrial DNA replication

2015 ◽  
Vol 112 (30) ◽  
pp. 9334-9339 ◽  
Author(s):  
J. Bradley Holmes ◽  
Gokhan Akman ◽  
Stuart R. Wood ◽  
Kiran Sakhuja ◽  
Susana M. Cerritelli ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Dna Replication ◽  
Strand Break ◽  
Initiation Site ◽  
Major Noncoding Region ◽  
Mitochondrial Dna Replication ◽  
Strand Initiation ◽  
Mitochondrial Dna Polymerase ◽  
Dna Maintenance

Encoding ribonuclease H1 (RNase H1) degrades RNA hybridized to DNA, and its function is essential for mitochondrial DNA maintenance in the developing mouse. Here we define the role of RNase H1 in mitochondrial DNA replication. Analysis of replicating mitochondrial DNA in embryonic fibroblasts lacking RNase H1 reveals retention of three primers in the major noncoding region (NCR) and one at the prominent lagging-strand initiation site termed Ori-L. Primer retention does not lead immediately to depletion, as the persistent RNA is fully incorporated in mitochondrial DNA. However, the retained primers present an obstacle to the mitochondrial DNA polymerase γ in subsequent rounds of replication and lead to the catastrophic generation of a double-strand break at the origin when the resulting gapped molecules are copied. Hence, the essential role of RNase H1 in mitochondrial DNA replication is the removal of primers at the origin of replication.

scholarly journals Mitochondrial DNA replication during differentiation of murine embryonic stem cells

2007 ◽  
Vol 120 (22) ◽  
pp. 4025-4034 ◽  
Author(s):  
J. M. Facucho-Oliveira ◽  
J. Alderson ◽  
E. C. Spikings ◽  
S. Egginton ◽  
J. C. St. John
Keyword(s):  
Stem Cells ◽  
Mitochondrial Dna ◽  
Dna Replication ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Murine Embryonic Stem Cells ◽  
Mitochondrial Dna Replication
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