scholarly journals Characterization of mouse embryonic stem cell differentiation into the pancreatic lineage in vitro by transcriptional profiling, quantitative RT-PCR and immunocytochemistry

2010 ◽  
Vol 54 (1) ◽  
pp. 41-54 ◽  
Author(s):  
Alexandra Rolletschek ◽  
Insa S. Schroeder ◽  
Herbert Schulz ◽  
Oliver Hummel ◽  
Norbert Huebner ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Transcriptional Profiling ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Stem Cell Differentiation ◽  
Embryonic Stem Cell Differentiation ◽  
Rt Pcr

scholarly journals RNA regulates Glycolysis and Embryonic Stem Cell Differentiation via Enolase 1

2020 ◽  
Author(s):  
Ina Huppertz ◽  
Joel I. Perez-Perri ◽  
Panagiotis Mantas ◽  
Thileepan Sekaran ◽  
Thomas Schwarzl ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cell ◽  
Rna Binding ◽  
Cultured Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Stem Cell Differentiation ◽  
Embryonic Stem Cell Differentiation

AbstractCells must coordinate their metabolism and fate trajectories (1, 2), but the underlying mechanisms are only beginning to be discovered. To understand why the glycolytic enzyme enolase 1 (ENO1) binds RNA (3–6), we studied this phenomenon in vitro, in human cells, and during mouse embryonic stem cell differentiation. We find specific cellular RNA ligands that inhibit ENO1’s enzymatic activity in vitro. Increasing the concentration of these ligands in cultured cells inhibits glycolysis. We demonstrate that pluripotent stem cells expressing an ENO1 mutant that is hyper-inhibited by RNA are severely impaired in their glycolytic capacity and in endodermal differentiation, whereas cells with an RNA binding-deficient ENO1 mutant display disproportionately high endodermal marker expression. Our findings uncover ENO1 riboregulation as a novel form of metabolic control. They also describe an unprecedented mechanism involved in the regulation of stem cell differentiation.One Sentence SummaryRNA directly regulates enzyme activity to control metabolism and stem cell fate

scholarly journals Activation of proHGF by St14 induces mouse embryonic stem cell differentiation

2016 ◽  
Vol 7 (8) ◽  
pp. 601-605
Author(s):  
Xiaoshuang Yan ◽  
Yan Xue ◽  
Yiye Zhou ◽  
Yan Cheng ◽  
Shang Yin ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Stem Cell Differentiation ◽  
Embryonic Stem Cell Differentiation

8-Hydroxyguanine levels and repair capacity during mouse embryonic stem cell differentiation

2011 ◽  
Vol 45 (5) ◽  
pp. 527-533 ◽  
Author(s):  
Ayumi Kuboyama ◽  
Shoichi Tanaka ◽  
Kazuaki Kawai ◽  
Hiroshi Kasai ◽  
Haruki Morii ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Differentiation ◽  
Embryonic Stem Cell ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cell ◽  
Stem Cell Differentiation ◽  
Embryonic Stem Cell Differentiation ◽  
Repair Capacity
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