scholarly journals Changes in E2F5 intracellular localization in mouse and human choroid plexus epithelium with development

2005 ◽  
Vol 49 (7) ◽  
pp. 859-865 ◽  
Author(s):  
Adam Swetloff ◽  
Patrizia Ferretti
Keyword(s):  
Choroid Plexus ◽  
Intracellular Localization ◽  
Choroid Plexus Epithelium ◽  
Human Choroid Plexus

Distribution of sodium transporters and aquaporin-1 in the human choroid plexus

2006 ◽  
Vol 291 (1) ◽  
pp. C59-C67 ◽  
Author(s):  
Jeppe Praetorius ◽  
Søren Nielsen
Keyword(s):  
Plasma Membrane ◽  
Choroid Plexus ◽  
Water Channel ◽  
Apical Plasma Membrane ◽  
Membrane Domain ◽  
Choroid Plexus Epithelium ◽  
Human Choroid Plexus ◽  
Fluid Production ◽  
Species Pattern ◽  
Plasma Membrane Domain

The choroid plexus epithelium secretes electrolytes and fluid in the brain ventricular lumen at high rates. Several channels and ion carriers have been identified as likely mediators of this transport in rodent choroid plexus. This study aimed to map several of these proteins to the human choroid plexus. Immunoperoxidase-histochemistry was employed to determine the cellular and subcellular localization of the proteins. The water channel, aquaporin (AQP) 1, was predominantly situated in the apical plasma membrane domain, although distinct basolateral and endothelial immunoreactivity was also observed. The Na+-K+-ATPase α1-subunit was exclusively localized apically in the human choroid plexus epithelial cells. Immunoreactivity for the Na+-K+-2Cl− cotransporter, NKCC1, was likewise confined to the apical plasma membrane domain of the epithelium. The Cl−/HCO3− exchanger, AE2, was localized basolaterally, as was the Na+-dependent Cl−/HCO3− exchanger, NCBE, and the electroneutral Na+-HCO3− cotransporter, NBCn1. No immunoreactivity was found toward the Na+-dependent acid/base transporters NHE1 or NBCe2. Hence, the human choroid plexus epithelium displays an almost identical distribution pattern of water channels and Na+ transporters as the rat and mouse choroid plexus. This general cross species pattern suggests central roles for these transporters in choroid plexus functions such as cerebrospinal fluid production.

Ultrastructural and immunohistological evidence for dendritic-like cells within human choroid plexus epithelium

Neuroreport ◽  
1997 ◽  
Vol 8 (8) ◽  
pp. 1995-1998 ◽  
Author(s):  
Jean-Marie Serot ◽  
Bernard Foliguet ◽  
Marie-Christine Béné ◽  
Gilbert-Charles Faure
Keyword(s):  
Choroid Plexus ◽  
Choroid Plexus Epithelium ◽  
Human Choroid Plexus

Organization of tight junctions in the choroid plexus epithelium

1978 ◽  
Vol 36 (2) ◽  
pp. 336-337
Author(s):  
B. Van Deurs ◽  
J. K. Koehler
Keyword(s):  
Choroid Plexus ◽  
Present Report ◽  
Freeze Fracture ◽  
Barrier Properties ◽  
Cell Junctions ◽  
Structural Basis ◽  
Apical Surface ◽  
Choroid Plexus Epithelium ◽  
Solid Nitrogen ◽  
Special Composition

The choroid plexus epithelium constitutes a blood-cerebrospinal fluid (CSF) barrier, and is involved in regulation of the special composition of the CSF. The epithelium is provided with an ouabain-sensitive Na/K-pump located at the apical surface, actively pumping ions into the CSF. The choroid plexus epithelium has been described as “leaky” with a low transepithelial resistance, and a passive transepithelial flux following a paracellular route (intercellular spaces and cell junctions) also takes place. The present report describes the structural basis for these “barrier” properties of the choroid plexus epithelium as revealed by freeze fracture.Choroid plexus from the lateral, third and fourth ventricles of rats were used. The tissue was fixed in glutaraldehyde and stored in 30% glycerol. Freezing was performed either in liquid nitrogen-cooled Freon 22, or directly in a mixture of liquid and solid nitrogen prepared in a special vacuum chamber. The latter method was always used, and considered necessary, when preparations of complementary (double) replicas were made.

scholarly journals T-Lymphocytes Traffic into the Brain across the Blood-CSF Barrier: Evidence Using a Reconstituted Choroid Plexus Epithelium

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0150945 ◽  
Author(s):  
Nathalie Strazielle ◽  
Rita Creidy ◽  
Christophe Malcus ◽  
José Boucraut ◽  
Jean-François Ghersi-Egea
Keyword(s):  
T Lymphocytes ◽  
Choroid Plexus ◽  
Choroid Plexus Epithelium ◽  
The Brain

scholarly journals Choroid plexus papillomas are induced by c-Myc overexpression in the choroid plexus via a T-cell inflammatory mechanism

2019 ◽  
Vol 21 (Supplement_4) ◽  
pp. iv9-iv10
Author(s):  
Ashirwad Merve ◽  
Xinyu Zhang ◽  
Nicola Pomella ◽  
Serena Acquati ◽  
Joerg Hoeck ◽  
...  
Keyword(s):  
T Cell ◽  
Mouse Model ◽  
Choroid Plexus ◽  
Tumour Size ◽  
Choroid Plexus Papilloma ◽  
Experimental Models ◽  
Chemotherapeutic Agents ◽  
Human Choroid Plexus ◽  
Choroid Plexus Papillomas ◽  
Plexus Papilloma

Abstract Choroid plexus tumours (CPT) account for up to 20% of brain tumours in children under 2 years of age. Histologically CPTs are classified into three categories - Choroid Plexus Papilloma (CPP), Atypical Choroid Plexus Papilloma (ACPP) and Choroid Plexus Carcinoma (CPC). Recent literature demonstrates that CPP and ACPP are molecularly distinct from CPC. Initial management for CPT include surgery followed by adjuvant therapy in selected patients. Currently there are no disease-specific chemotherapeutic agents available, possibly because of their rarity and paucity of faithful pre-clinical experimental models. In this study we show that c-Myc overexpression in the choroid plexus epithelium induces T-cell inflammation-dependent choroid plexus papillomas in a mouse model. We demonstrate that c-MYC is expressed in a substantial proportion of human choroid plexus tumours and that this subgroup of tumours is characterised by an inflammatory transcriptome and significant inflammatory infiltrates. We observed that triple transgenic compound mutant mouse model with c-Myc overexpression in an immune-suppressed background led to a decreased incidence of CPP and reduced tumour bulk. A reduced tumour size was also observed when c-Myc overexpressing mice were treated with anti-CD3 antibodies. Our data raise the possibility that benign choroid plexus tumours expressing c-MYC could be amenable to medical therapy with anti-inflammatory drugs.

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