scholarly journals Vasculogenesis and angiogenesis in the mouse embryo studied using quail/mouse chimeras

2005 ◽  
Vol 49 (2-3) ◽  
pp. 355-361 ◽  
Author(s):  
Michel Pudliszewski ◽  
Luc Pardanaud
Keyword(s):  
Mouse Embryo ◽  
Mouse Chimeras ◽  
Vasculogenesis And Angiogenesis

Spontaneous Production of Type C Virus Particles in a Culture Derived from Rat Embryo Cells

1972 ◽  
Vol 30 ◽  
pp. 288-289
Author(s):  
Elizabeth S. Priori ◽  
T. Shigematsu ◽  
B. Myers ◽  
L. Dmochowski
Keyword(s):  
Virus Particle ◽  
Mouse Embryo ◽  
Calf Serum ◽  
Embryo Cell ◽  
Virus Particles ◽  
Extracellular Virus ◽  
Mouse Embryo Cell ◽  
Mature Virus Particle ◽  
Embryo Cells ◽  
Type C

Spontaneous release of type C virus particles in long-term cultures of mouse embryo cells as well as induction of similar particles in mouse embryo cell cultures with IUDR or BUDR have been reported. The presence of type C virus particles in cultures of normal rat embryos has not been reported.NB-1, a culture derived from embryos of a New Zealand Black (NB) rat (rats obtained from Mr. Samuel M. Poiley, N.C.I., Bethesda, Md.) and grown in McCoy's 5A medium supplemented with 20% fetal calf serum was passaged weekly. Extracellular virus particles similar to murine leukemia particles appeared in the 22nd subculture. General appearance of cells in passage 23 is shown in Fig. 1. Two budding figures and one immature type C virus particle may be seen in Fig. 2. The virus particles and budding were present in all further passages examined (currently passage 39). Various stages of budding are shown in Figs. 3a,b,c,d. Appearance of a mature virus particle is shown in Fig. 4.

Use of F9 teratocarcinoma STEM cells to study cell-matrix interactions in the early mouse embryo

1992 ◽  
Vol 50 (1) ◽  
pp. 602-603
Author(s):  
Marc Lenburg ◽  
Rulang Jiang ◽  
Lengya Cheng ◽  
Laura Grabel
Keyword(s):  
Mouse Embryo ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Cell Types ◽  
Embryoid Bodies ◽  
F9 Cells ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Cell Matrix Interactions ◽  
F9 Teratocarcinoma

We are interested in defining the cell-cell and cell-matrix interactions that help direct the differentiation of extraembryonic endoderm in the peri-implantation mouse embryo. At the blastocyst stage the mouse embryo consists of an outer layer of trophectoderm surrounding the fluid-filled blastocoel cavity and an eccentrically located inner cell mass. On the free surface of the inner cell mass, facing the blastocoel cavity, a layer of primitive endoderm forms. Primitive endoderm then generates two distinct cell types; parietal endoderm (PE) which migrates along the inner surface of the trophectoderm and secretes large amounts of basement membrane components as well as tissue-type plasminogen activator (tPA), and visceral endoderm (VE), a columnar epithelial layer characterized by tight junctions, microvilli, and the synthesis and secretion of α-fetoprotein. As these events occur after implantation, we have turned to the F9 teratocarcinoma system as an in vitro model for examining the differentiation of these cell types. When F9 cells are treated in monolayer with retinoic acid plus cyclic-AMP, they differentiate into PE. In contrast, when F9 cells are treated in suspension with retinoic acid, they form embryoid bodies (EBs) which consist of an outer layer of VE and an inner core of undifferentiated stem cells. In addition, we have established that when VE containing embryoid bodies are plated on a fibronectin coated substrate, PE migrates onto the matrix and this interaction is inhibited by RGDS as well as antibodies directed against the β1 integrin subunit. This transition is accompanied by a significant increase in the level of tPA in the PE cells. Thus, the outgrowth system provides a spatially appropriate model for studying the differentiation and migration of PE from a VE precursor.

Cell-matrix interactions in the early mouse embryo

1992 ◽  
Vol 50 (1) ◽  
pp. 600-601
Author(s):  
A.E. Sutherland ◽  
P.G. Calarco ◽  
C.H. Damsky
Keyword(s):  
Mouse Embryo ◽  
Giant Cells ◽  
Blastocyst Stage ◽  
Integrin Subunit ◽  
Β1 Integrin ◽  
Cell Stage ◽  
Early Mouse Embryo ◽  
Migratory Activity ◽  
Early Mouse ◽  
Cell Matrix Interactions

Cell-extracellular matrix (ECM) interactions mediated by the integrin family of receptors are critical for morphogenesis and may also play a regulatory role in differentiation during early development. We have examined the onset of expression of individual integrin subunit proteins in the early mouse embryo, and their roles in early morphogenetic events. As detected by immunoprecipitation, the α6, αV, β1, and β3 subunits are detected as early as the 4-cell stage, α5 at the hatched blastocyst stage and αl and α3 following blastocyst attachment. We tested the role of these integrins in the attachment and migratory activity of two cell populations of the early mouse embryo: the trophoblast giant cells, which invade the uterine stroma and ultimately contribute to the chorio-allantoic placenta, and the parietal endoderm, which migrates over the inner surface of the trophoblast and ultimately forms Reichert's membrane and the parietal yolk sac. Experiments were done in serum-free medium on substrates coated with laminin (Ln) and fibronectin (Fn). Trophoblast outgrowth occurs on Ln and its E8 fragment (long arm), but not on the E1’ fragment (cross region) (Figs. 1, 2 ). This outgrowth is inhibited by anti-E8, anti-Ln, and by the anti-β1 family antiserum anti-ECMR, but not by anti-αV or the function-perturbing GoH3 antibody that recognizes the α6/β1 integrin, a major Ln (E8) receptor. This suggests that trophoblast outgrowth on Ln or E8 is mediated by a different β1 integrin such as α3/β1. Early stages of trophoblast outgrowth (up to 48 hours) on Fn are inhibited by anti-Fn and by function-perturbing anti-αV antibodies, whereas at later times outgrowth becomes insensitive to anti-αV but remains sensitive to the anti-β1 family antiserum anti-ECMr, indicating that trophoblast cells modulate their interaction with Fn during outgrowth. Trophoblast outgrowth on vitronectin (Vn) is sensitive to anti-αV antibodies throughout the 5-day period examined.

Vasculogenesis and Angiogenesis: Lessons From Genetics in Mice

2002 ◽  
Vol 7 (6) ◽  
pp. 381-416
Author(s):  
Rita L. S. Carvalho ◽  
Mariëtte H. E. Driessens ◽  
Christine Mummery
Keyword(s):  
Vasculogenesis And Angiogenesis

Transporters for L-proline in the pre-implantation mouse embryo

2014 ◽  
Author(s):  
Margot L Day ◽  
M Zada ◽  
Charles Bailey ◽  
Tamara Treleaven ◽  
Sukran Ozsoy ◽  
...  
Keyword(s):  
Mouse Embryo

Importance of amino acids in the development of preimplantation mouse embryo

2014 ◽  
Author(s):  
Radu Zamfirescu ◽  
Salini Shreedharan ◽  
Mark Zada ◽  
Michael Morris ◽  
Margot L Day
Keyword(s):  
Amino Acids ◽  
Mouse Embryo ◽  
Preimplantation Mouse Embryo ◽  
Preimplantation Mouse

A New Concept of the Pathogenesis of Retinopathy of Prematurity

2020 ◽  
pp. 5-12
Author(s):  
E.I. Sidorenko ◽  
Keyword(s):  
Growth Factors ◽  
Retinopathy Of Prematurity ◽  
Vascular Endothelium ◽  
Retinal Vessels ◽  
Protective Mechanisms ◽  
Preclinical Stage ◽  
Circulatory Hypoxia ◽  
Preclinical Phase ◽  
Vasculogenesis And Angiogenesis ◽  
Vascular Autoregulation

The lecture provides a detailed description of the development of retinal vessels, vasculogenesis and angiogenesis. The cascade of protective mechanisms of the organism of the system of combating hyperoxia, the system of combating circulatory hypoxia and the key growth factors of the vascular endothelium during angiogenesis are shown. The pathogenesis of retinopathy of prematurity is described and the first phase of ROP development is analyzed in detail, the reasons for the delay in the maturation of vascular autoregulation are explained. The author proposed to distinguish the first preclinical phase in the ROP classification. In contrast to the modern concentration of attention of ophthalmologists on the active and cicatricial stages, the author proposes to pay special attention to the study of the preclinical stage of ROP, where its pathogenesis is formed.

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