Molecular Characterization of Dopamine D2 Receptor Isoforms Tagged With Green Fluorescent Protein

2006 ◽  
Vol 34 (1) ◽  
pp. 1-14 ◽  
Author(s):  
Keyvan Sedaghat ◽  
Marie-France Nantel ◽  
Simon Ginsberg ◽  
Véronique Lalonde ◽  
Mario Tiberi
Keyword(s):  
Green Fluorescent Protein ◽  
Molecular Characterization ◽  
Fluorescent Protein ◽  
Dopamine D2 Receptor ◽  
D2 Receptor ◽  
Dopamine D2 ◽  
Receptor Isoforms ◽  
Green Fluorescent

scholarly journals Preferential Coupling of Dopamine D2S and D2L Receptor Isoforms with Gi1 and Gi2 Proteins—In Silico Study

2020 ◽  
Vol 21 (2) ◽  
pp. 436 ◽  
Author(s):  
Justyna Żuk ◽  
Damian Bartuzi ◽  
Dariusz Matosiuk ◽  
Agnieszka A. Kaczor
Keyword(s):  
Dopamine D2 Receptor ◽  
D2 Receptor ◽  
Antipsychotic Agents ◽  
Intracellular Loop ◽  
Dopamine D2 ◽  
Receptor Isoforms ◽  
In Silico Study ◽  
Third Intracellular Loop ◽  
G Protein Coupled ◽  
Insight Into

The dopamine D2 receptor belongs to rhodopsin-like G protein-coupled receptors (GPCRs) and it is an important molecular target for the treatment of many disorders, including schizophrenia and Parkinson’s disease. Here, computational methods were used to construct the full models of the dopamine D2 receptor short (D2S) and long (D2L) isoforms (differing with 29 amino acids insertion in the third intracellular loop, ICL3) and to study their coupling with Gi1 and Gi2 proteins. It was found that the D2L isoform preferentially couples with the Gi2 protein and D2S isoform with the Gi1 protein, which is in accordance with experimental data. Our findings give mechanistic insight into the interplay between isoforms of dopamine D2 receptors and Gi proteins subtypes, which is important to understand signaling by these receptors and their mediation by pharmaceuticals, in particular psychotic and antipsychotic agents.

Toward Molecular-Level Characterization of Photoinduced Decarboxylation of the Green Fluorescent Protein: Accessibility of the Charge-Transfer States

2012 ◽  
Vol 8 (6) ◽  
pp. 1912-1920 ◽  
Author(s):  
Bella L. Grigorenko ◽  
Alexander V. Nemukhin ◽  
Dmitry I. Morozov ◽  
Igor V. Polyakov ◽  
Ksenia B. Bravaya ◽  
...  
Keyword(s):  
Charge Transfer ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Molecular Level ◽  
Green Fluorescent
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