Sex Differences in Lumbar Spinal Cord Gene Expression Following Experimental Lumbar Radiculopathy

2006 ◽  
Vol 30 (3) ◽  
pp. 283-296 ◽  
Author(s):  
Michael L. LaCroix-Fralish ◽  
Vivianne L. Tawfik ◽  
Kevin F. Spratt ◽  
Joyce A. DeLeo
Keyword(s):  
Gene Expression ◽  
Spinal Cord ◽  
Sex Differences ◽  
Lumbar Spinal Cord ◽  
Lumbar Radiculopathy ◽  
Lumbar Spinal

Differential Spinal Cord Gene Expression in Rodent Models of Radicular and Neuropathic Pain

2006 ◽  
Vol 104 (6) ◽  
pp. 1283-1292 ◽  
Author(s):  
Michael L. LaCroix-Fralish ◽  
Vivianne L. Tawfik ◽  
Flobert Y. Tanga ◽  
Kevin F. Spratt ◽  
Joyce A. DeLeo
Keyword(s):  
Gene Expression ◽  
Spinal Cord ◽  
Chronic Pain ◽  
Polymerase Chain Reaction ◽  
Mechanical Allodynia ◽  
Lumbar Spinal Cord ◽  
Chain Reaction ◽  
Pain Models ◽  
Polymerase Chain ◽  
Lumbar Spinal

Background Neuropathic pain and radicular low back pain both have a major impact on human health worldwide. Microarray gene analysis on central nervous system tissues holds great promise for discovering novel targets for persistent pain modulation. Methods Rat models of lumbar radiculopathy (L5 nerve root ligation) and neuropathy (L5 spinal nerve transection) were used for these studies. The authors measured mechanical allodynia followed by analysis of global gene expression in the lumbar spinal cord at two time points (7 days and 14 days) after surgery using the Affymetrix RAE230A GeneChip(R) (Santa Clara, CA). The expression patterns of several genes of interest were subsequently confirmed using real-time reverse transcriptase polymerase chain reaction. Results The authors observed similarly robust mechanical allodynia in both models. Second, they observed significant differences in lumbar spinal cord gene expression across chronic pain models. There was little overlap between genes altered in each injury model, suggesting that the site and type of injury produce distinct spinal mechanisms mediating the observed mechanical allodynia. The authors further confirmed a subset of the genes using reverse transcriptase polymerase chain reaction and identified several genes as either neuropathy-associated genes or radiculopathy-associated genes. Conclusions These two models of persistent pain produce similar allodynic outcomes but produce differential gene expression. These results suggest that diverging mechanisms lead to a common behavioral outcome in these pain models. Furthermore, these distinct pathophysiologic mechanisms in neuropathic versus radicular pain may implicate unique drug therapies for these types of chronic pain syndromes.

Differential effects of left and right neuropathy on opioid gene expression in lumbar spinal cord

2018 ◽  
Vol 1695 ◽  
pp. 78-83 ◽  
Author(s):  
Olga Kononenko ◽  
Irina Mityakina ◽  
Vladimir Galatenko ◽  
Hiroyuki Watanabe ◽  
Igor Bazov ◽  
...  
Keyword(s):  
Gene Expression ◽  
Spinal Cord ◽  
Lumbar Spinal Cord ◽  
Differential Effects ◽  
Left And Right ◽  
Lumbar Spinal

Genomic study of gene expression in the mouse lumbar spinal cord under the conditions of simulated microgravity

2011 ◽  
Vol 439 (1) ◽  
pp. 197-200 ◽  
Author(s):  
R. R. Islamov ◽  
A. A. Rizvanov ◽  
O. V. Tyapkina ◽  
B. S. Shenkman ◽  
I. B. Kozlovskaya ◽  
...  
Keyword(s):  
Gene Expression ◽  
Spinal Cord ◽  
Simulated Microgravity ◽  
Lumbar Spinal Cord ◽  
Genomic Study ◽  
Lumbar Spinal

scholarly journals Integrative genetic analysis of the amyotrophic lateral sclerosis spinal cord implicates glial activation and suggests new risk genes

2021 ◽  
Author(s):  
Jack Humphrey ◽  
Sanan Venkatesh ◽  
Rahat Hasan ◽  
Jake T Herb ◽  
Katia de Paiva Lopes ◽  
...  
Keyword(s):  
Gene Expression ◽  
Spinal Cord ◽  
Amyotrophic Lateral Sclerosis ◽  
Motor Neurons ◽  
Cell Types ◽  
Lumbar Spinal Cord ◽  
Gene Expression Response ◽  
Expression Response ◽  
Lumbar Spinal ◽  
Lateral Sclerosis

Amyotrophic lateral sclerosis (ALS) is a progressively fatal neurodegenerative disease affecting motor neurons in the brain and spinal cord. We used 380 post-mortem tissue RNA-seq transcriptomes from 154 ALS cases and 49 control individuals from cervical, thoracic, and lumbar spinal cord segments to investigate the gene expression response to ALS. We observed an increase in microglia and astrocyte expression, accompanied by a decrease in oligodendrocytes. By creating a gene co-expression network in the ALS samples, we identify several activated microglia modules that negatively correlate with retrospective disease duration. We map molecular quantitative trait loci and find several potential ALS risk loci that may act through gene expression or splicing in the spinal cord and assign putative cell-types for FNBP1, ACSL5, SH3RF1 and NFASC. Finally, we outline how repeat expansions that alter splicing of C9orf72 are tagged by common variants, and use this to suggest ATXN3 as a putative risk gene.

scholarly journals Expression of changes in NT3 gene after rat's sciatica nerve compression in the presence of hydroalcoholic extract of mountain tea (Stachyslavandulifolia)

2020 ◽  
Author(s):  
Fariba Ghasemzadeh ◽  
Maryam Tehranipour ◽  
Khadijeh Nezhad Shahrokhabadi
Keyword(s):  
Gene Expression ◽  
Spinal Cord ◽  
Male Rats ◽  
Lumbar Spinal Cord ◽  
Control Group ◽  
Alcoholic Extract ◽  
Hydroalcoholic Extract ◽  
Treatment Groups ◽  
Soxhlet Method ◽  
Lumbar Spinal

Introduction: Neurotrophic factors change in response to nerve damage. Stachyslavandulifolia belongs to the Laminaceae family and since tea has antioxidant and anti-inflammatory effects, therefore, this study aimed to determine the effects of hydroalcoholic extract of mountain tea and its effect on NT3 gene expression after compression. Methods: In this experimental study, at first the hydro-alcoholic extract of stachys was prepared by the Soxhlet method. In this study, 36 Wistar male rats , 250-300 gr, were randomly divided into 9 groups, 4 rats in each group, and included control, compression (1, 7, 14 and 21 days) and experimental (1, 7, 14 and 28 days) groups. Experimental groups were treated by 75 mg / kg of hydro-alcoholic extract of stachys and to induce the stress in the control group, saline serum was injected. In compression and experimental groups , the sciatic nerve of right leg was compressed for 60 seconds. The first injection of extract in experimental group was performed intraperitoneally and immediately after the compression and the second one was injected 7 days later. Then the sampling was performed of lumbar spinal cord on 1, 7, 14 and 28 days in compression and experimental groups and the total RNA was extracted from the spinal cord segments, cDNA was synthesized and after that the alteration of gene expression of NT3 samples was studied in both samples, without treatment and treated with hydro-alcoholic extract.Data were analyzed using MxPro software and Anova test with a significant level of p <0.05 and Excel software was used for drawing graphs. Results: The results showed that NT3 gene expression was significantly increased in the compression and treatment groups (p <0.001). Although, the NT3 gene expression was decreased in the treatment group compared to the compression group. Conclusion: It seems that hydroalcoholic extract of Stachyslavandulifolia shoots did not affect NT3 gene expression.

Effect of 17β-estradiol on gene expression in lumbar spinal cord following sciatic nerve crush injury in ovariectomized mice

2003 ◽  
Vol 966 (1) ◽  
pp. 65-75 ◽  
Author(s):  
Rustem R. Islamov ◽  
Wesley A. Hendricks ◽  
Laxmansa C. Katwa ◽  
Roger J. McMurray ◽  
Elena S. Pak ◽  
...  
Keyword(s):  
Gene Expression ◽  
Spinal Cord ◽  
Sciatic Nerve ◽  
Lumbar Spinal Cord ◽  
Sciatic Nerve Crush ◽  
Nerve Crush ◽  
Ovariectomized Mice ◽  
17Β Estradiol ◽  
Lumbar Spinal ◽  
Nerve Crush Injury

Full-genome study of gene expression in lumbar spinal cord of mice after 30-day space flight on Bion-M1 biosatellite

2016 ◽  
Vol 122 ◽  
pp. 231-236 ◽  
Author(s):  
R.R. Islamov ◽  
О.А. Gusev ◽  
A. Tanabe ◽  
M. Terada ◽  
O.V. Tyapkina ◽  
...  
Keyword(s):  
Gene Expression ◽  
Spinal Cord ◽  
Space Flight ◽  
Lumbar Spinal Cord ◽  
Full Genome ◽  
Genome Study ◽  
Lumbar Spinal

scholarly journals Neonatal androgen-dependent sex differences in lumbar spinal cord dopamine concentrations and the number of A11 diencephalospinal dopamine neurons

2010 ◽  
pp. NA-NA ◽  
Author(s):  
Samuel S. Pappas ◽  
Chelsea T. Tiernan ◽  
Bahareh Behrouz ◽  
Cynthia L. Jordan ◽  
S. Marc Breedlove ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Sex Differences ◽  
Dopamine Neurons ◽  
Lumbar Spinal Cord ◽  
Lumbar Spinal
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