Cytokine Gene Expression in Peripheral Blood Mononuclear Cells Reflects a Systemic Immune Response in Alcoholic Chronic Pancreatitis

1999 ◽  
Vol 26 (3) ◽  
pp. 137-146 ◽  
Author(s):  
Christoph Hanck ◽  
Siegbert Rossol ◽  
Annette Hartmann ◽  
Manfred V. Singer
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Chronic Pancreatitis ◽  
Mononuclear Cells ◽  
Cytokine Gene Expression ◽  
Cytokine Gene ◽  
Systemic Immune Response ◽  
Peripheral Blood Mononuclear ◽  
Alcoholic Chronic Pancreatitis ◽  
Blood Mononuclear Cells

scholarly journals Cytokine Gene Expression Occurs More Rapidly in Stimulated Peripheral Blood Mononuclear Cells from Human Immunodeficiency Virus-Infected Persons

2000 ◽  
Vol 7 (5) ◽  
pp. 769-773 ◽  
Author(s):  
Elizabeth Crabb Breen ◽  
Matthew McDonald ◽  
Jiang Fan ◽  
John Boscardin ◽  
John L. Fahey
Keyword(s):  
Gene Expression ◽  
Mononuclear Cells ◽  
Cytokine Gene Expression ◽  
Cytokine Gene ◽  
Cytokine Mrna ◽  
Peripheral Blood Mononuclear ◽  
Tnf Α ◽  
Immunodeficiency Virus ◽  
Blood Mononuclear Cells ◽  
Kinetics Of

ABSTRACT Evaluation of cytokine gene expression following in vitro stimulation is one means of examining the dysregulation of the immune system in human immunodeficiency virus (HIV) infection. We have assessed differences in the immune status of non-HIV-infected (HIV−) and HIV-infected (HIV+) individuals by evaluating the kinetics of the expression of cytokine genes. We compared detailed time courses of cytokine mRNA expression in HIV− and HIV+ peripheral blood mononuclear cells (PBMC) and found that there is a significant shift (P < 0.01) for all cytokines examined (interleukin 2 [IL-2], IL-6, IL-10, gamma interferon, and tumor necrosis factor alpha [TNF-α]) to an earlier time of mean peak mRNA expression by HIV+ PBMC (between 4 and 8 h) compared to HIV− PBMC (8 h) in response to either phytohemagglutinin (PHA) or anti-CD3 stimulation. Additional studies showed that although PHA-stimulated HIV+ PBMC showed decreased median IL-2, IL-4, and TNF-α mRNA levels, they typically demonstrated more rapid kinetics (increased mean 4-h/24-h cytokine mRNA ratios), with significant differences for IL-4 (P < 0.05) and TNF-α (P < 0.005), compared to HIV− PBMC. The use of fresh or frozen cells gave comparable cytokine mRNA data; however, the secretion of some cytokine proteins (IL-2 receptor, IL-10, and TNF-α) appeared to be reduced in HIV+ PBMC that had been frozen and thawed. Our studies demonstrate that the kinetics of cytokine gene expression can reveal additional dysregulation of the immune system in HIV infection, suggesting that PBMC of HIV-infected persons exist in an activated state in vivo that permits them to express cytokine genes more rapidly than a normal PBMC.

Sign in / Sign up

Export Citation Format

Share Document