Plasma Concentrations of Ir-inhibin, Inhibin A, Inhibin pro-αC, FSH, and Estradiol-17β During Estrous Cycle in Mares and Their Relationship with Follicular Growth

Endocrine ◽  
2004 ◽  
Vol 25 (1) ◽  
pp. 07-14 ◽  
Author(s):  
Mohamed S. Medan ◽  
Yasuo Nambo ◽  
Natsuko Nagamine ◽  
Hiromi Shinbo ◽  
Gen Watanabe ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Plasma Concentrations ◽  
Follicular Growth ◽  
Inhibin A ◽  
Estradiol 17Β

scholarly journals Peripheral concentrations of inhibin A, ovarian steroids, and gonadotropins associated with follicular development throughout the estrous cycle of the sow

Reproduction ◽  
2010 ◽  
Vol 139 (1) ◽  
pp. 153-161 ◽  
Author(s):  
Michiko Noguchi ◽  
Koji Yoshioka ◽  
Seigo Itoh ◽  
Chie Suzuki ◽  
Sachiko Arai ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Luteal Phase ◽  
Inverse Relationship ◽  
Plasma Concentrations ◽  
Follicular Development ◽  
Follicular Phase ◽  
Follicular Growth ◽  
Inhibin A ◽  
Ovarian Steroids ◽  
Feedback Regulator

We investigated changes in peripheral concentrations of inhibin A, total inhibin, steroids, and gonadotropins throughout the intact estrous cycle of the sow in relation to ovarian changes determined by daily transrectal ultrasonography. All visible follicles of 3 mm or more in diameter were classified as small (≥3 and <6 mm) or large (≥6 mm). Follicular recruitment was identified in two periods of the cycle: one from the late luteal to the follicular phase, characterized by an increase in the number of small follicles followed by the appearance of large follicles; and another during the early luteal phase, consisting only of increased numbers of small follicles. Plasma concentrations of inhibin A increased (P<0.05), coinciding with the two periods of follicle emergence. Estradiol (E2) levels increased (P<0.05) during the follicular phase, but not during the early luteal phase. An inverse relationship (P<0.01) between the patterns of inhibin and FSH concentrations was noted around the two periods of follicle emergence, but there was no relationship (P≥0.1) between the patterns of plasma E2and FSH during the early luteal phase. In conclusion, measurement of plasma inhibin A levels combined with ultrasonographic examination of the ovaries revealed two periods of synchronous follicular growth during the sow's estrous cycle. The results strongly suggest that inhibin A functions as a negative feedback regulator of FSH secretion throughout the estrous cycle, whereas E2appears to influence FSH secretion only during the follicular phase.

Changes in Plasma Concentrations of Inhibin, Estradiol-17β, Progesterone, LH and FSH Associated with Follicular Waves During the Estrous Cycle in Sows.

2008 ◽  
Vol 78 (Suppl_1) ◽  
pp. 103-104
Author(s):  
Michiko Noguchi ◽  
Koji Yoshioka ◽  
Chie Suzuki ◽  
Sachiko Arai ◽  
Seigo Itoh ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Plasma Concentrations ◽  
Follicular Waves ◽  
Estradiol 17Β

scholarly journals Plasma level and expression of visfatin in the porcine hypothalamus during the estrous cycle and early pregnancy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tadeusz Kaminski ◽  
Marta Kiezun ◽  
Ewa Zaobidna ◽  
Kamil Dobrzyn ◽  
Barbara Wasilewska ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Estrous Cycle ◽  
Early Pregnancy ◽  
Energy Homeostasis ◽  
Plasma Concentrations ◽  
Tissue Samples ◽  
Hormone Synthesis ◽  
Energy Sensor ◽  
Gene And Protein Expression ◽  
Hormonal Milieu

AbstractVisfatin appears to be an energy sensor involved in the regulation of female fertility, which creates a hormonal link integrating the control of energy homeostasis and reproduction. This study evaluates the expression levels of visfatin gene and protein in selected areas of the porcine hypothalamus responsible for gonadotropin-releasing hormone synthesis: the mediobasal hypothalamus (MBH) and preoptic area (POA), and visfatin concentrations in the blood plasma. The tissue samples were harvested from gilts on days 2–3, 10–12, 14–16, and 17–19 of the estrous cycle, and on days 10–11, 12–13, 15–16, 27–28 of pregnancy. Visfatin was localized in the cytoplasm and nucleus of cells creating both studied hypothalamic structures. The study demonstrated that visfatin gene and protein expression in MBH and POA depends on hormonal status related to the phase of the estrous cycle or early pregnancy. Blood plasma concentrations of visfatin during the estrous cycle were higher on days 2–3 in relation to other studied phases of the cycle, while during early pregnancy, the highest visfatin contents were observed on days 12–13. This study demonstrated visfatin expression in the porcine hypothalamus and its dependence on the hormonal milieu related to the estrous cycle and early pregnancy.

scholarly journals Relationship between plasma concentrations of estradiol-17β and progesterone and the distribution of epithelial and inflammatory cells in the external opening of the river buffaloes’ uterine cervix during the gestational period

2021 ◽  
Vol 24 (3) ◽  
pp. 365-375
Author(s):  
M. Goli ◽  
E. Ayen ◽  
S. Hassanzadeh ◽  
M. H. Khadem Anssari
Keyword(s):  
Uterine Cervix ◽  
Jugular Vein ◽  
Plasma Concentrations ◽  
Inflammatory Cells ◽  
Gestation Period ◽  
Blood Levels ◽  
External Opening ◽  
Microscopic Evaluation ◽  
The Difference ◽  
Estradiol 17Β

Changes in the distribution of the epithelial and inflammatory cells in the external opening of the uterine cervix in river buffaloes at three different occasions during the gestation period were investigated by light microscopic evaluation of mucus smears prepared by wet swab sampling and Giemsa-stained. Forty five pregnant river buffaloes that were in the first (n=15), second (n=15) and third (n=15) approximately equal parts of their gestation period were sampled twice concurrently by individual wet swabs from the external opening of the uterine cervix. Five smears were prepared from each sample. Immediately after mucus sampling, blood samples were obtained from the jugular vein of each animal, centrifuged immediately and stored at -20 oC to assay the plasma levels of estradiol-17β and progesterone. The results showed that changes in the percentage of the vacuolated and non-vacuolated epithelial cells, lymphocytes, eosinophils, and basophils were not statistically significant between the groups, which suggests that as the pregnancy progressed, changes in the blood levels of estradiol-17β and progesterone had little or no effect on the distribution of these cells in the region, but changes in the percentage of neutrophils were statistically significant between the groups. It was also found that changes in the percentage of macrophages between the second and third trimesters of the gestation period were not significant, but the difference between the first and the other trimesters was statistically significant. It was concluded that the percentages of neutrophils and macrophages increase significantly as the plasma concentrations of progesterone decrease during the gestation.

scholarly journals Plasma Level and Expression of Visfatin in the Porcine Hypothalamus During the Estrous Cycle and Early Pregnancy

2020 ◽  
Author(s):  
Tadeusz Kaminski ◽  
Marta Kiezun ◽  
Ewa Zaobidna ◽  
Kamil Dobrzyń ◽  
Barbara Wasilewska ◽  
...  
Keyword(s):  
Protein Expression ◽  
Blood Plasma ◽  
Estrous Cycle ◽  
Early Pregnancy ◽  
Energy Homeostasis ◽  
Plasma Concentrations ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tissue Samples ◽  
Hormone Synthesis ◽  
Gene And Protein Expression

Abstract BackgroundVisfatin exists in two forms: the intracellular form which is a rate limiting enzyme engaged in nicotinamide adenine dinucleotide biosynthesis and the extracellular form considered as an adipokine, produced mainly by the adipose tissue. Visfatin could be an energy sensor involved in regulating female fertility, which creates a hormonal link integrating the control of energy homeostasis and reproduction. MethodsThe study compares the expression levels of visfatin gene (quantitative real time PCR) and protein (Western blotting and fluorescent immunohistochemistry) in the selected areas of the porcine hypothalamus responsible for gonadotropin releasing hormone synthesis: the mediobasal hypothalamus (MBH) and preoptic area (POA), and visfatin concentrations in the blood plasma (enzyme-linked immunosorbent assay). The tissue samples were harvested from gilts on days 2-3, 10-12, 14-16 and 17-19 of the estrous cycle, and on days 10-11, 12-13, 15-16, 27-28 of pregnancy. Differences between groups were analyzed by one-way ANOVA followed by Tukey’s post hoc test. ResultsDuring the estrous cycle, visfatin mRNA expression in the MBH was higher on days 2-3 and 17-19, while the visfatin protein concentration on days 17-19. During early pregnancy, the most pronounced gene and protein expression in the MBH was found on days 15-16 and 10-11, respectively. In the POA during the estrous cycle, visfatin gene expression was the highest on days 17-19, and the protein level of visfatin on days 14-16. During early pregnancy, the highest expression of visfatin gene in the POA was observed on days 15-16, whereas the protein concentrations on days 27-28. Blood plasma concentrations of visfatin during the estrous cycle were higher on days 2-3 in relation to other studied phases of the cycle. During early pregnancy, the highest visfatin contents in the blood plasma were observed on days 12-13. Visfatin gene and protein expression in MBH and POA, and visfatin plasma concentrations differed during early pregnancy in relation to days 10-12 of the cycle. ConclusionsThis study demonstrated visfatin expression in the porcine hypothalamus and its dependence on hormonal milieu related to the estrous cycle and early pregnancy.

A selective increase in circulating inhibin and inhibin pro-αC at the time of ovulation in the mare

1999 ◽  
Vol 277 (5) ◽  
pp. E870-E875 ◽  
Author(s):  
Kentaro Nagaoka ◽  
Yasuo Nambo ◽  
Natsuko Nagamine ◽  
Shun-Ichi Nagata ◽  
Yumiko Tanaka ◽  
...  
Keyword(s):  
Follicular Fluid ◽  
Plasma Concentrations ◽  
Precursor Protein ◽  
Α Subunit ◽  
Ovulatory Follicles ◽  
The Relationship ◽  
Selective Increase ◽  
Estradiol 17Β

The relationship between a selective increase in circulating immunoreactive (ir)-inhibin and the time of ovulation was investigated in mares. Concentrations of plasma ir-inhibin were measured every 4 h during the periovulatory period. Inhibin pro-αC, a precursor protein of the inhibin α-subunit, was also measured. The changes in ir-inhibin and inhibin pro-αC in circulation were parallel. Concentrations of both ir-inhibin and inhibin pro-αC in the plasma increased at the same time when ovulatory follicles ruptured, and the peak levels of circulating ir-inhibin and inhibin pro-αC were maintained for 4–8 h. There was no selective increase in plasma concentrations of estradiol-17β during the process of ovulation. These results suggest that the selective increase in ir-inhibin and inhibin pro-αC was caused by the absorption of follicular fluid after the rupture of ovulatory follicles. These results also suggest that the measuring of plasma concentrations of ir-inhibin or inhibin pro-αC in mares might be a useful method for detecting the time of ovulation.

scholarly journals Changes in Plasma Concentrations of Immunoreactive Inhibin, Estradiol and FSH Associated with Follicular Waves during the Estrous Cycle of the Cow

1995 ◽  
Vol 41 (4) ◽  
pp. 311-320 ◽  
Author(s):  
Hiroyuki KANEKO ◽  
Hisashi KISHI ◽  
Gen WATANABE ◽  
Kazuyoshi TAYA ◽  
Shuji SASAMOTO ◽  
...  
Keyword(s):  
Estrous Cycle ◽  
Plasma Concentrations ◽  
Follicular Waves

scholarly journals Dihydrotestosterone influenced numbers of healthy follicles and follicular amounts of LH receptor mRNA during the follicular phase of the estrous cycle in gilts

Reproduction ◽  
2008 ◽  
Vol 135 (3) ◽  
pp. 343-350 ◽  
Author(s):  
H Cárdenas ◽  
E Jiménez ◽  
W F Pope
Keyword(s):  
Estrous Cycle ◽  
Corn Oil ◽  
Follicular Phase ◽  
Lh Receptor ◽  
Receptor Mrna ◽  
Gonadotropin Receptor ◽  
Treatment Dose ◽  
Recruitment And Selection ◽  
Estradiol 17Β ◽  
Receptor Inhibitor

The present experiments were conducted to determine androgenic effects on numbers, health, and amounts of gonadotropin receptor mRNA in late developing follicles of gilts. Gilts (n=5 per group) received daily injections of one of the following treatments on days 13–16 or days 13–18 of the estrous cycle: corn oil, 5α-dihydrotestosterone (DHT, 10 mg), flutamide (1.5 g, an androgen receptor inhibitor), DHT (10 mg) plus flutamide (1.5 g), testosterone (10 mg), and testosterone (10 mg) plus flutamide (1.5 g). Ovarian follicles ≥5 mm in diameter were evaluated on day 17 or 19, 24 h after receiving the last treatment dose. Follicles were classified as healthy (H), moderately atretic (MA), or very atretic (VA). Treatment with DHT increased (P<0.05) the numbers of H follicles relative to control gilts on days 17 and 19. DHT administration from days 13 to 16 diminished (P<0.05) the amounts of LH receptor (LHR) mRNA in H follicles from day 17 (relative amounts: 1.45±0.33 and 2.72±0.33 for DHT- and vehicle-treated gilts respectively). The effects of DHT on numbers of H follicles and LHR mRNA were not observed in gilts receiving DHT plus flutamide. Androgens did not influence numbers of MA, VA, and total follicles, or follicular estradiol-17β concentrations and amounts of FSHR mRNA. Treating gilts with DHT during follicular recruitment and selection did not induce changes in the numbers of total follicles ≥5 mm, but rather increased the numbers of healthy follicles in this follicular population in association with decreased amounts of LHR mRNA.

scholarly journals Secretion of inhibin A, inhibin B and inhibin pro-alphaC during the oestrous cycle of the golden hamster (Mesocricetus auratus)

1999 ◽  
Vol 162 (3) ◽  
pp. 451-456 ◽  
Author(s):  
K Ohshima ◽  
H Kishi ◽  
M Itoh ◽  
G Watanabe ◽  
K Arai ◽  
...  
Keyword(s):  
Golden Hamster ◽  
Plasma Concentrations ◽  
Follicular Development ◽  
Early Morning ◽  
Oestrous Cycle ◽  
Inhibin B ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inhibin A ◽  
Negative Relationships ◽  
Plasma Oestradiol

Plasma concentrations of inhibin pro-alphaC, inhibin A and inhibin B were determined by enzyme-linked immunosorbent assay at 6 h intervals throughout the 4-day oestrous cycle of the golden hamster. Plasma concentrations of follicle-stimulating hormone (FSH) and oestradiol-17beta were also measured by radioimmunoassay during the oestrous cycle. Plasma concentrations of inhibin A increased from the early morning of day 1 (day 1=day of ovulation) and reached plateau levels at 0500 h on day 2. An abrupt increase in plasma concentrations of inhibin A was found at 1700 h on day 4, when the preovulatory FSH surge was observed. An increase in plasma concentrations of inhibin B occurred on day 1 and reached plateau levels at 1700 h on day 1. The levels remained elevated until 0500 h on day 4 and declined gradually by 2300 h on day 4. Plasma concentrations of inhibin pro-alphaC gradually increased with some fluctuation from day 1 to 1700 h on day 4 and then declined. Significant negative relationships were noted between plasma FSH and both dimeric forms of inhibin from day 1 to day 3. Significant positive relationships were found between plasma oestradiol-17beta and inhibin A or inhibin pro-alphaC throughout the oestrous cycle. In contrast, no significant relationship was found between plasma oestradiol-17beta and inhibin B. These findings suggest that both dimeric forms of inhibin play a role in the regulation of FSH secretion during follicular development. These findings also suggest that inhibin pro-alphaC could be secreted primarily by large follicles, and early atretic follicles could also be responsible for inhibin pro-alphaC secretion. On the other hand, the secretory pattern of dimeric inhibins might shift from inhibin B to inhibin A with follicular development.

Sign in / Sign up

Export Citation Format

Share Document