Hydrogenase 3 But Not Hydrogenase 4 is Major in Hydrogen Gas Production by Escherichia coli Formate Hydrogenlyase at Acidic pH and in the Presence of External Formate

2004 ◽  
Vol 41 (3) ◽  
pp. 357-366 ◽  
Author(s):  
Nelli Mnatsakanyan ◽  
Karine Bagramyan ◽  
Armen Trchounian
Keyword(s):  
Escherichia Coli ◽  
Gas Production ◽  
Hydrogen Gas ◽  
Acidic Ph ◽  
Formate Hydrogenlyase ◽  
Hydrogenase 3

scholarly journals Molecular Hydrogen Formation by Escherichia Coli Hydrogenase 3 during Fermentation of Glucose at Slightly Acidic pH

2011 ◽  
Vol 100 (3) ◽  
pp. 488a
Author(s):  
Anna Poladyan ◽  
Anna Poghosyan ◽  
Karen Trchounian ◽  
Armen Trchounian
Keyword(s):  
Escherichia Coli ◽  
Molecular Hydrogen ◽  
Acidic Ph ◽  
Hydrogen Formation ◽  
Hydrogenase 3

Enhanced hydrogen gas production from mixture of beer spent grains (BSG) and distiller's grains (DG) with glycerol by Escherichia coli

2020 ◽  
Vol 45 (35) ◽  
pp. 17233-17240 ◽  
Author(s):  
Satenik Mirzoyan ◽  
Akerke Toleugazykyzy ◽  
Kairat Bekbayev ◽  
Armen Trchounian ◽  
Karen Trchounian
Keyword(s):  
Escherichia Coli ◽  
Gas Production ◽  
Hydrogen Gas ◽  
Distiller's Grains ◽  
Spent Grains

scholarly journals The dual-function chaperone HycH improves assembly of the formate hydrogenlyase complex

2017 ◽  
Vol 474 (17) ◽  
pp. 2937-2950 ◽  
Author(s):  
Ute Lindenstrauß ◽  
Philipp Skorupa ◽  
Jennifer S. McDowall ◽  
Frank Sargent ◽  
Constanze Pinske
Keyword(s):  
Escherichia Coli ◽  
Electron Transfer ◽  
Mutant Strain ◽  
Protein Complexes ◽  
Dual Function ◽  
Formate Hydrogenlyase ◽  
Accessory Function ◽  
Membrane Bound ◽  
Hydrogenase 3 ◽  
Influence Activity

The assembly of multi-protein complexes requires the concerted synthesis and maturation of its components and subsequently their co-ordinated interaction. The membrane-bound formate hydrogenlyase (FHL) complex is the primary hydrogen-producing enzyme in Escherichia coli and is composed of seven subunits mostly encoded within the hycA-I operon for [NiFe]-hydrogenase-3 (Hyd-3). The HycH protein is predicted to have an accessory function and is not part of the final structural FHL complex. In this work, a mutant strain devoid of HycH was characterised and found to have significantly reduced FHL activity due to the instability of the electron transfer subunits. HycH was shown to interact specifically with the unprocessed species of HycE, the catalytic hydrogenase subunit of the FHL complex, at different stages during the maturation and assembly of the complex. Variants of HycH were generated with the aim of identifying interacting residues and those that influence activity. The R70/71/K72, the Y79, the E81 and the Y128 variant exchanges interrupt the interaction with HycE without influencing the FHL activity. In contrast, FHL activity, but not the interaction with HycE, was negatively influenced by H37 exchanges with polar residues. Finally, a HycH Y30 variant was unstable. Surprisingly, an overlapping function between HycH with its homologous counterpart HyfJ from the operon encoding [NiFe]-hydrogenase-4 (Hyd-4) was identified and this is the first example of sharing maturation machinery components between Hyd-3 and Hyd-4 complexes. The data presented here show that HycH has a novel dual role as an assembly chaperone for a cytoplasmic [NiFe]-hydrogenase.

scholarly journals Dose–Response Effects of 3-Nitrooxypropanol Combined with Low- and High-Concentrate Feed Proportions in the Dairy Cow Ration on Fermentation Parameters in a Rumen Simulation Technique

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1784
Author(s):  
Matthias Schilde ◽  
Dirk von Soosten ◽  
Liane Hüther ◽  
Susanne Kersten ◽  
Ulrich Meyer ◽  
...  
Keyword(s):  
Dose Response ◽  
Gas Production ◽  
Hydrogen Gas ◽  
Simulation Technique ◽  
Mitigation Strategies ◽  
Feed Additive ◽  
Future Research ◽  
Dependent Manner ◽  
Dose Dependent

Methane (CH4) from ruminal feed degradation is a major pollutant from ruminant livestock, which calls for mitigation strategies. The purpose of the present 4 × 2 factorial arrangement was to investigate the dose–response relationships between four doses of the CH4 inhibitor 3-nitrooxypropanol (3-NOP) and potential synergistic effects with low (LC) or high (HC) concentrate feed proportions (CFP) on CH4 reduction as both mitigation approaches differ in their mode of action (direct 3-NOP vs. indirect CFP effects). Diet substrates and 3-NOP were incubated in a rumen simulation technique to measure the concentration and production of volatile fatty acids (VFA), fermentation gases as well as substrate disappearance. Negative side effects on fermentation regarding total VFA and gas production as well as nutrient degradability were observed for neither CFP nor 3-NOP. CH4 production decreased from 10% up to 97% in a dose-dependent manner with increasing 3-NOP inclusion rate (dose: p < 0.001) but irrespective of CFP (CFP × dose: p = 0.094). Hydrogen gas accumulated correspondingly with increased 3-NOP dose (dose: p < 0.001). In vitro pH (p = 0.019) and redox potential (p = 0.066) varied by CFP, whereas the latter fluctuated with 3-NOP dose (p = 0.01). Acetate and iso-butyrate (mol %) decreased with 3-NOP dose, whereas iso-valerate increased (dose: p < 0.001). Propionate and valerate varied inconsistently due to 3-NOP supplementation. The feed additive 3-NOP was proven to be a dose-dependent yet effective CH4 inhibitor under conditions in vitro. The observed lack of additivity of increased CFP on the CH4 inhibition potential of 3-NOP needs to be verified in future research testing further diet types both in vitro and in vivo.

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