Simultaneous Purification and Reversible Immobilization of D-Amino Acid Oxidase from Trigonopsis variabilis on a Hydrophobic Support

2001 ◽  
Vol 95 (2) ◽  
pp. 083-092 ◽  
Author(s):  
Stanislaus F D'Souza ◽  
Ashwini Deshpande
1985 ◽  
Vol 31 (7) ◽  
pp. 625-628 ◽  
Author(s):  
Eva M. Kubicek-Pranz ◽  
M. Röhr

Production of D-amino-acid oxidase by Trigonopsis variabilis has been investigated using a two-stage cultivation technique. After transfer of exponentially growing cells to fresh medium, the enzyme was induced by addition of D-amino acids to the growth medium, among which D-methionine and D-alanine were the most effective. The simultaneous presence of the L form of amino acids or [Formula: see text] did not affect this induction. The presence of trace metals, inorganic phosphate, and a high rate of agitation were necessary for formation of maximal D-amino-acid oxidase activity. The enzyme is not subject to glucose repression.


1962 ◽  
Vol 27 (3) ◽  
pp. 465-471 ◽  
Author(s):  
S. Sentheshanmuganathan ◽  
W. J. Nickerson

1980 ◽  
Vol 33 (6) ◽  
pp. 585-590 ◽  
Author(s):  
NOBUFUSA SERIZAWA ◽  
KEIKO NAKAGAWA ◽  
TATSUO HANEISHI ◽  
SHOJI KAMIMURA ◽  
ATSUSHI NAITO

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