Metabolic Engineering of Saccharomyces cerevisiae for Efficient Production of Pure L-(+)-Lactic Acid

2006 ◽  
Vol 131 (1-3) ◽  
pp. 795-807 ◽  
Author(s):  
Nobuhiro Ishida ◽  
Satoshi Saitoh ◽  
Toru Ohnishi ◽  
Kenro Tokuhiro ◽  
Eiji Nagamori ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Metabolic Engineering ◽  
Efficient Production

Metabolic engineering of Saccharomyces cerevisiae for efficient production of pure l−(+)−lactic acid

1996 ◽  
Vol 131 (1-3) ◽  
pp. 795-807 ◽  
Author(s):  
Nobuhiro Ishida ◽  
Satoshi Saitoh ◽  
Toru Ohnishi ◽  
Kenro Tokuhiro ◽  
Eiji Nagamori ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Metabolic Engineering ◽  
Efficient Production

scholarly journals Combinatorial Metabolic Engineering in Saccharomyces cerevisiae for the Enhanced Production of the FPP-Derived Sesquiterpene Germacrene

2020 ◽  
Vol 7 (4) ◽  
pp. 135
Author(s):  
Jan Niklas Bröker ◽  
Boje Müller ◽  
Dirk Prüfer ◽  
Christian Schulze Gronover
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering ◽  
Metabolic Flux ◽  
Mevalonate Pathway ◽  
Plant Secondary Metabolites ◽  
Product Formation ◽  
Culture Conditions ◽  
Efficient Production ◽  
Enhanced Production ◽  
Engineering Strategy

Farnesyl diphosphate (FPP)-derived isoprenoids represent a diverse group of plant secondary metabolites with great economic potential. To enable their efficient production in the heterologous host Saccharomyces cerevisiae, we refined a metabolic engineering strategy using the CRISPR/Cas9 system with the aim of increasing the availability of FPP for downstream reactions. The strategy included the overexpression of mevalonate pathway (MVA) genes, the redirection of metabolic flux towards desired product formation and the knockout of genes responsible for competitive reactions. Following the optimisation of culture conditions, the availability of the improved FPP biosynthesis for downstream reactions was demonstrated by the expression of a germacrene synthase from dandelion. Subsequently, biosynthesis of significant amounts of germacrene-A was observed in the most productive strain compared to the wild type. Thus, the presented strategy is an excellent tool to increase FPP-derived isoprenoid biosynthesis in yeast.

Metabolic engineering of Saccharomyces cerevisiae for efficient production of endocrocin and emodin

2019 ◽  
Vol 54 ◽  
pp. 212-221 ◽  
Author(s):  
Lei Sun ◽  
Guiyou Liu ◽  
Ya Li ◽  
Dayong Jiang ◽  
Wenfeng Guo ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering ◽  
Efficient Production

scholarly journals The Model System Saccharomyces cerevisiae Versus Emerging Non-Model Yeasts for the Production of Biofuels

Life ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 299
Author(s):  
Maria Priscila Lacerda ◽  
Eun Joong Oh ◽  
Carrie Eckert
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Metabolic Engineering ◽  
Synthetic Biology ◽  
Biofuel Cell ◽  
Efficient Production ◽  
Yeast Saccharomyces Cerevisiae ◽  
Fatty Acid Production ◽  
Inhibitor Tolerance ◽  
Cell Factories ◽  
Recent Advances

Microorganisms are effective platforms for the production of a variety of chemicals including biofuels, commodity chemicals, polymers and other natural products. However, deep cellular understanding is required for improvement of current biofuel cell factories to truly transform the Bioeconomy. Modifications in microbial metabolic pathways and increased resistance to various types of stress caused by the production of these chemicals are crucial in the generation of robust and efficient production hosts. Recent advances in systems and synthetic biology provide new tools for metabolic engineering to design strategies and construct optimal biocatalysts for the sustainable production of desired chemicals, especially in the case of ethanol and fatty acid production. Yeast is an efficient producer of bioethanol and most of the available synthetic biology tools have been developed for the industrial yeast Saccharomyces cerevisiae. Non-conventional yeast systems have several advantageous characteristics that are not easily engineered such as ethanol tolerance, low pH tolerance, thermotolerance, inhibitor tolerance, genetic diversity and so forth. Currently, synthetic biology is still in its initial steps for studies in non-conventional yeasts such as Yarrowia lipolytica, Kluyveromyces marxianus, Issatchenkia orientalis and Pichia pastoris. Therefore, the development and application of advanced synthetic engineering tools must also focus on these underexploited, non-conventional yeast species. Herein, we review the basic synthetic biology tools that can be applied to the standard S. cerevisiae model strain, as well as those that have been developed for non-conventional yeasts. In addition, we will discuss the recent advances employed to develop non-conventional yeast strains that are efficient for the production of a variety of chemicals through the use of metabolic engineering and synthetic biology.

Improvement of d ‐Lactic Acid Production in Saccharomyces cerevisiae Under Acidic Conditions by Evolutionary and Rational Metabolic Engineering

2017 ◽  
Vol 12 (10) ◽  
pp. 1700015 ◽  
Author(s):  
Seung‐Ho Baek ◽  
Eunice Y. Kwon ◽  
Sang‐Jeong Bae ◽  
Bo‐Ram Cho ◽  
Seon‐Young Kim ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Metabolic Engineering ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Acidic Conditions
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