Manipulating the Phenolic Acid Content and Digestibility of Italian Ryegrass (Lolium multiflorum) by Vacuolar-Targeted Expression of a Fungal Ferulic Acid Esterase

2006 ◽  
Vol 130 (1-3) ◽  
pp. 415-426 ◽  
Author(s):  
Marcia M. De O. Buanafina ◽  
Tim Langdon ◽  
Barbara Hauck ◽  
Sue J. Dalton ◽  
Phil Morris
Keyword(s):  
Ferulic Acid ◽  
Phenolic Acid ◽  
Acid Content ◽  
Lolium Multiflorum ◽  
Italian Ryegrass ◽  
Ferulic Acid Esterase ◽  
Targeted Expression

scholarly journals Isolation of Ferulic Acid from Leaves of Mindi (Melia azedarach L.) and Its Antioxidant Activity Test

2018 ◽  
Vol 3 (1) ◽  
pp. 30
Author(s):  
Andriyani Budi Listyo ◽  
Dewi Kusrini ◽  
Enny Fachriyah
Keyword(s):  
Antioxidant Activity ◽  
Ferulic Acid ◽  
Phenolic Acid ◽  
Acid Content ◽  
Ethanol Extract ◽  
Bioactive Compound ◽  
Base Hydrolysis ◽  
Melia Azedarach ◽  
Dpph Radical ◽  
Activity Test

<p>Mindi (<em>Melia azedarach </em>L.) is one of a plant from <em>Meliaceae </em>family and has been known for its bioactive compound, such as ferulic acid. On this study, the isolation of ferulic acid and antioxidant activity test has been conducted. The aim of this study was to isolate the ferulic acid on ethanol extract of <em>Melia azedarach </em>L. leaves, determine the ferulic acid content and antioxidant activity test qualitatively. The method used in this research was isolation with hydrolysis (acid and base) and without hydrolysis, determining the phenolic acid content using TLC scanner instrument and antioxidant activity test qualitatively using DPPH radical reduction method. According to the identification using TLC and TLC scanner, the base hydrolysis fraction (HB), acid hydrolysis (HA), and without hydrolysis (HA) of ethanol extract from <em>Melia azedarach </em>L. was suspected contain the ferulic acid compound. The analysis result using TLC scanner was known to have ferulic acid content on HB, HA, and TH fraction of 15.57%,12.17%, and 9.56%, respectively. This study showed that hydrolysis affected the phenolic acid content where the HB fraction produced higher ferulic acid than HA and TH fraction. The antioxidant activity test that has been conducted qualitatively showed that ferulic acid isolates contained on each fraction actively reduce the DPPH radical.</p>

CHARACTERIZATION OF LIGNIN-CARBOHYDRATE COMPLEXES OF ITALIAN RYEGRASS AND ALFALFA

1990 ◽  
Vol 70 (1) ◽  
pp. 193-201 ◽  
Author(s):  
TSUNEO KONDO ◽  
KAZUHIKO MIZUNO ◽  
TADASHI KATO ◽  
TADAKAZU HIROI
Keyword(s):  
Enzymatic Hydrolysis ◽  
Ferulic Acid ◽  
Enzymatic Degradation ◽  
Produced Water ◽  
Lolium Multiflorum ◽  
Complex Structure ◽  
Italian Ryegrass ◽  
Syringyl Lignin ◽  
Insoluble Material ◽  
Ferulic Acids

Lignin-carbohydrate complexes (LCC) were isolated from Italian ryegrass (Lolium multiflorum Lam.) and alfalfa (Medicago sativa L.) stems and their chemical and physical properties, and enzymatic degradation examined. The LCCs, soluble in water, were composed of guaiacyl-syringyl lignin and hemicellulosic carbohydrate mostly consisting of xylose, glucose and arabinose. Enzymatic hydrolysis of the LCCs produced water-insoluble materials which were markedly rich in lignin. In both plant species, the carbohydrate of the insoluble material had a high proportion of arabinose. The insoluble material of Italian ryegrass contained two to three times more xylose and arabinose than that of alfalfa. The Italian ryegrass LCC released significant amounts of p-coumaric and ferulic acids by alkaline and acid hydrolysis, but the alfalfa LCC did not, indicating a more complex structure of the cell wall matrix of Italian ryegrass. The ferulic acid in ryegrass lignin may be responsible for restricting enzymatic degradation of hemicellulose in ryegrass more than occurs in legumes.Key words: Lignin-carbohydrate complex, enzymatic hydrolysis, hemicellulose, ferulic acid, Italian ryegrass, alfalfa

scholarly journals Phenolic acids in kernels of different coloured-grain wheat genotypes

2020 ◽  
Vol 66 (No. 2) ◽  
pp. 57-64 ◽  
Author(s):  
Luboš Paznocht ◽  
Zora Kotíková ◽  
Barbora Burešová ◽  
Jaromír Lachman ◽  
Petr Martinek
Keyword(s):  
Ferulic Acid ◽  
Phenolic Acids ◽  
Phenolic Acid ◽  
Acid Content ◽  
High Performance ◽  
Sinapic Acid ◽  
Triticum Aestivum L ◽  
Total Phenolic ◽  
Array Detector ◽  
The Individual

The content of free, conjugated and bound phenolic acids in 12 wheat (Triticum aestivum L.) genotypes of 4 different grain colours (standard red, yellow endosperm, purple pericarp and blue aleurone) from 2-year field trial was analysed in the present study. Significant increase (8%) in the total phenolic acid content was observed in the dryer year 2017. Five phenolic acids (ferulic, sinapic, p-coumaric, vanillic and 4-hydroxybenzoic) and cis-isomers of ferulic and sinapic acid were determined by HPLC-DAD (high-performance liquid chromatography with a diode-array detector) in grain samples. The total phenolic acid content of coloured wheat groups varied: blue aleurone &gt; purple pericarp &gt; yellow endosperm &gt; red colour (798 &gt; 702 &gt; 693 &gt; 599 µg/g). The fraction of bound phenolic acids was the major contributor to the total phenolic acid content (91.7%) with ferulic acid predominating (85.2%). Conjugated phenolic acids accounted for 7.9% of the total with sinapic and ferulic acid predominating (47.6% and 19.9%). The composition of individual phenolic acids was similar within these two fractions. The remaining 0.4% was represented by the fraction of free phenolic acids in which the phenolic acid profile varied among the individual coloured groups. Ferulic acid prevailed in red and yellow wheats, vanillic in blue and p-coumaric in purple wheats.  

CELL WALL-BOUND p-COUMARIC AND FERULIC ACIDS IN ITALIAN RYEGRASS

1990 ◽  
Vol 70 (2) ◽  
pp. 495-499 ◽  
Author(s):  
TSUNEO KONDO ◽  
KAZUHIKO MIZUNO ◽  
TADASHI KATO
Keyword(s):  
Cell Wall ◽  
Ferulic Acid ◽  
Lolium Multiflorum ◽  
Italian Ryegrass ◽  
Coumaric Acid ◽  
Stages Of Growth ◽  
Cell Wall Polymers ◽  
Cross Links ◽  
Ferulic Acids ◽  
Acid Esters

Cell wall-bound p-coumaric and ferulic acids were determined for stems of Italian ryegrass (Lolium multiflorum Lam.) sampled at different stages of growth. The greater part of the detected p-coumaric acid was linked to cell wall polymers through ester linkages. No definite relationship was found between the accumulation of p-coumaric acid esters and plant maturation. Some part of the detected ferulic acid was simultaneously esterified and etherified to cell wall polymers. Such ester- and ether-linked ferulic acid augmented with increasing maturity, suggesting that the number of ferulic acid cross-links in the cell wall matrices of ryegrass increases with plant maturation.Key words: p-coumaric acid, ferulic acid, Italian ryegrass, cell wall, phenolic acids

Inhibitory effects of Italian ryegrass (Lolium multiflorum Lam.) seedlings of rice (Oryza sativa L.)

2018 ◽  
Vol 44 (2) ◽  
pp. 219-232 ◽  
Author(s):  
S.J Jang ◽  
K.R. Kim ◽  
Y.B. Yun ◽  
S.S. Kim ◽  
Y.I Kuk
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Lolium Multiflorum ◽  
Italian Ryegrass ◽  
Inhibitory Effects

Quantitative trait loci analysis of nitrate-nitrogen content in Italian ryegrass (Lolium multiflorum Lam.)

Euphytica ◽  
2021 ◽  
Vol 217 (1) ◽  
Author(s):  
Wenqing Tan ◽  
Di Zhang ◽  
Nana Yuyama ◽  
Jun Chen ◽  
Shinichi Sugita ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Nitrogen Content ◽  
Quantitative Trait ◽  
Nitrate Nitrogen ◽  
Lolium Multiflorum ◽  
Italian Ryegrass ◽  
Trait Loci

Protection of Italian ryegrass (Lolium multiflorum L.) seedlings from salinity stress following seed priming with L-methionine and casein hydrolysate

2020 ◽  
pp. 1-9
Author(s):  
Keum-Ah Lee ◽  
Youngnam Kim ◽  
Hossein Alizadeh ◽  
David W.M. Leung
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Salinity Stress ◽  
Lolium Multiflorum ◽  
Casein Hydrolysate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Italian Ryegrass ◽  
Significant Difference ◽  
Protein Amino Acids

Abstract Seed priming with water (hydropriming or HP) has been shown to be beneficial for seed germination and plant growth. However, there is little information on the effects of seed priming with amino acids and casein hydrolysate (CH) compared with HP, particularly in relation to early post-germinative seedling growth under salinity stress. In this study, Italian ryegrass seeds (Lolium multiflorum L.) were primed with 1 mM of each of the 20 protein amino acids and CH (200 mg l−1) before they were germinated in 0, 60 and 90 mM NaCl in Petri dishes for 4 d in darkness. Germination percentage (GP), radicle length (RL) and peroxidase (POD) activity in the root of 4-d-old Italian ryegrass seedlings were investigated. Generally, when the seeds were germinated in 0, 60 and 90 mM NaCl, there was no significant difference in GP of seeds among various priming treatments, except that a higher GP was observed in seeds of HP treatment compared with the non-primed seeds when incubated in 60 mM NaCl. When incubated in 60 and 90 mM NaCl, seedlings from seeds primed with L-methionine or CH exhibited greater RL (greater protection against salinity stress) and higher root POD activity than those from non-primed and hydro-primed seeds. Under salinity stress, there were higher levels of malondialdehyde (MDA) in the root of 4-d-old Italian ryegrass seedlings, a marker of oxidative stress, but seed priming with CH was effective in reducing the salinity-triggered increase in MDA content. These results suggest that priming with L-methionine or CH would be better than HP for the protection of seedling root growth under salinity stress and might be associated with enhanced antioxidative defence against salinity-induced oxidative stress.

scholarly journals Characterization of the hydroxyproline-rich protein core of an arabinogalactan-protein secreted from suspension-cultured Lolium multiflorum (Italian ryegrass) endosperm cells

1989 ◽  
Vol 264 (3) ◽  
pp. 857-862 ◽  
Author(s):  
P A Gleeson ◽  
M McNamara ◽  
R E H Wettenhall ◽  
B A Stone ◽  
G B Fincher
Keyword(s):  
Polyclonal Antibodies ◽  
Lolium Multiflorum ◽  
Italian Ryegrass ◽  
Arabinogalactan Protein ◽  
Myeloma Protein ◽  
Protein Core ◽  
Liquid Suspension ◽  
Peptide Component ◽  
Immunodominant Epitopes

An arabinogalactan-protein (AGP) purified from the filtrate of liquid-suspension-cultured Italian-ryegrass (Lolium multiflorum) endosperm cells by affinity chromatography on myeloma protein J539-Sepharose was deglycosylated with trifluoromethanesulphonic acid to remove polysaccharide chains that are covalently associated with hydroxyproline residues in the peptide component of the proteoglycan. The protein core, which accounts for less than 10% (w/w) of the intact proteoglycan, was purified by h.p.l.c. It has an apparent Mr of 35,000, but reacts very poorly with both Coomassie Brilliant Blue R and silver stains. Amino-acid-sequence analysis of the N-terminus of the h.p.l.c.-purified protein core and of tryptic peptides generated from the unpurified protein reveals a high content of hydroxyproline and alanine. These are sometimes arranged in short (Ala-Hyp) repeat sequences of up to six residues. Polyclonal antibodies raised against the protein core do not cross-react with native AGP, the synthetic peptide (Ala-Hyp)4, poly-L-hydroxyproline or poly-L-proline. The results suggest that the polysaccharide chains in the native AGP render the protein core of the proteoglycan inaccessible to the antibodies and that the immunodominant epitopes include domains of the protein other than those rich in Ala-Hyp repeating units.

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