High-Cell-Density Cultivation of Pseudomonas putida IPT 046 and Medium-Chain-Length Polyhydroxyalkanoate Production From Sugarcane Carbohydrates

2004 ◽  
Vol 119 (1) ◽  
pp. 51-70 ◽  
Author(s):  
Simone Cardoso Diniz ◽  
Marilda Keico Taciro ◽  
José Gregório Cabrera Gomez ◽  
José Geraldo da Cruz Pradella
Keyword(s):  
Pseudomonas Putida ◽  
Cell Density ◽  
Chain Length ◽  
High Cell Density ◽  
High Cell ◽  
High Cell Density Cultivation ◽  
Medium Chain ◽  
Medium Chain Length

scholarly journals Development of High Cell Density Cultivation Strategies for Improved Medium Chain Length Polyhydroxyalkanoate Productivity Using Pseudomonas putida LS46

2019 ◽  
Vol 6 (4) ◽  
pp. 89 ◽  
Author(s):  
Warren Blunt ◽  
Christopher Dartiailh ◽  
Richard Sparling ◽  
Daniel J. Gapes ◽  
David B. Levin ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Cell Density ◽  
Chain Length ◽  
Octanoic Acid ◽  
High Cell Density ◽  
High Cell ◽  
Fed Batch ◽  
Medium Chain Length ◽  
Pulse Feed ◽  
Feed Strategy

High cell density (HCD) fed-batch cultures are widely perceived as a requisite for high-productivity polyhydroxyalkanoate (PHA) cultivation processes. In this work, a reactive pulse feed strategy (based on real-time CO2 or dissolved oxygen (DO) measurements as feedback variables) was used to control an oxygen-limited fed-batch process for improved productivity of medium chain length (mcl-) PHAs synthesized by Pseudomonas putida LS46. Despite the onset of oxygen limitation half-way through the process (14 h post inoculation), 28.8 ± 3.9 g L−1 total biomass (with PHA content up to 61 ± 8% cell dry mass) was reliably achieved within 27 h using octanoic acid as the carbon source in a bench-scale (7 L) bioreactor operated under atmospheric conditions. This resulted in a final volumetric productivity of 0.66 ± 0.14 g L−1 h−1. Delivering carbon to the bioreactor as a continuous drip feed process (a proactive feeding strategy compared to pulse feeding) made little difference on the final volumetric productivity of 0.60 ± 0.04 g L−1 h−1. However, the drip feed strategy favored production of non-PHA residual biomass during the growth phase, while pulse feeding favored a higher rate of mcl-PHA synthesis and yield during the storage phase. Overall, it was shown that the inherent O2-limitation brought about by HCD cultures can be used as a simple and effective control strategy for mcl-PHA synthesis from fatty acids. Furthermore, the pulse feed strategy appears to be a relatively easy and reliable method for rapid optimization of fed-batch processes, particularly when using toxic substrates like octanoic acid.

scholarly journals High Cell Density Conversion of Hydrolysed Waste Cooking Oil Fatty Acids Into Medium Chain Length Polyhydroxyalkanoate Using Pseudomonas putida KT2440

Catalysts ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 468 ◽  
Author(s):  
Carolina Ruiz ◽  
Shane T. Kenny ◽  
Ramesh Babu P ◽  
Meg Walsh ◽  
Tanja Narancic ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Pseudomonas Putida ◽  
Cell Density ◽  
High Cell Density ◽  
Waste Cooking Oil ◽  
High Cell ◽  
Pseudomonas Putida Kt2440 ◽  
Cooking Oil ◽  
Medium Chain Length ◽  
Feeding Experiments

Waste cooking oil (WCO) is a major pollutant, primarily managed through incineration. The high cell density bioprocess developed here allows for better use of this valuable resource since it allows the conversion of WCO into biodegradable polymer polyhydroxyalkanoate (PHA). WCO was chemically hydrolysed to give rise to a mixture of fatty acids identical to the fatty acid composition of waste cooking oil. A feed strategy was developed to delay the stationary phase, and therefore achieve higher final biomass and biopolymer (PHA) productivity. In fed batch (pulse feeding) experiments Pseudomonas putida KT2440 achieved a PHA titre of 58 g/l (36.4% of CDW as PHA), a PHA volumetric productivity of 1.93 g/l/h, a cell density of 159.4 g/l, and a biomass yield of 0.76 g/g with hydrolysed waste cooking oil fatty acids (HWCOFA) as the sole substrate. This is up to 33-fold higher PHA productivity compared to previous reports using saponified palm oil. The polymer (PHA) was sticky and amorphous, most likely due to the long chain monomers acting as internal plasticisers. High cell density cultivation is essential for the majority of industrial processes, and this bioprocess represents an excellent basis for the industrial conversion of WCO into PHA.

Fed-batch strategies using butyrate for high cell density cultivation of Pseudomonas putida and its use as a biocatalyst

2014 ◽  
Vol 98 (22) ◽  
pp. 9217-9228 ◽  
Author(s):  
Federico Cerrone ◽  
Gearoid Duane ◽  
Eoin Casey ◽  
Reeta Davis ◽  
Ian Belton ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Cell Density ◽  
High Cell Density ◽  
High Cell ◽  
High Cell Density Cultivation ◽  
Fed Batch

High cell density cultivation of anchorage-dependent cells using a novel macroporous cellulosic support

1992 ◽  
Vol 74 (1) ◽  
pp. 27-31 ◽  
Author(s):  
Tatsuya Ogawa ◽  
Masamichi Kamihira ◽  
Shuji Terashima ◽  
Kimiaki Yasuda ◽  
Shinji Iijima ◽  
...  
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
High Cell ◽  
High Cell Density Cultivation

Kinetics of medium-chain-length polyhydroxyalkanoate production by a novel isolate of Pseudomonas putida LS46

2012 ◽  
Vol 58 (8) ◽  
pp. 982-989 ◽  
Author(s):  
Parveen K. Sharma ◽  
Jilagamazhi Fu ◽  
Nazim Cicek ◽  
Richard Sparling ◽  
David B. Levin
Keyword(s):  
Pseudomonas Putida ◽  
Chain Length ◽  
Saturated Fatty Acids ◽  
Dry Mass ◽  
Nucleotide Sequence Analysis ◽  
Glucose Medium ◽  
Medium Chain ◽  
Medium Chain Length ◽  
Pha Production ◽  
Nitrogen Excess

Six bacteria that synthesize medium-chain-length polyhydroxyalkanoates (mcl-PHAs) were isolated from sewage sludge and hog barn wash and identified as strains of Pseudomonas and Comamonas by 16S rDNA gene sequencing. One isolate, Pseudomonas putida LS46, showed good PHA production (22% of cell dry mass) in glucose medium, and it was selected for further studies. While it is closely related to other P. putida strains (F1, KT2440, BIRD-1, GB-1, S16, and W619), P. putida LS46 was genetically distinct from these other strains on the basis of nucleotide sequence analysis of the cpn60 gene hypervariable region. PHA production was detected as early as 12 h in both nitrogen-limited and nitrogen-excess conditions. The increase in PHA production after 48 h was higher in nitrogen-limited cultures than in nitrogen-excess cultures. Pseudomonas putida LS46 produced mcl-PHAs when cultured with glucose, glycerol, or C6–C14 saturated fatty acids as carbon sources, and mcl-PHAs accounted for 56% of the cell dry mass when cells were batch cultured in medium containing 20 mmol/L octanoate. Although 3-hydroxydecanoate was the major mcl-PHA monomer (58.1–68.8 mol%) in P. putida LS46 cultured in glucose medium, 3-hydroxyoctanoate was the major monomer produced in octanoate medium (88 mol%).

scholarly journals Draft Genome Sequence of Medium-Chain-Length Polyhydroxyalkanoate-Producing Pseudomonas putida Strain LS46

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
P. K. Sharma ◽  
J. Fu ◽  
X. Zhang ◽  
B. W. Fristensky ◽  
K. Davenport ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Chain Length ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Medium Chain ◽  
Medium Chain Length

scholarly journals High cell density cultivation of a recombinant E. coli strain expressing a key enzyme in bioengineered heparin production

2013 ◽  
Vol 97 (9) ◽  
pp. 3893-3900 ◽  
Author(s):  
Odile Francesca Restaino ◽  
Ujjwal Bhaskar ◽  
Priscilla Paul ◽  
Lingyun Li ◽  
Mario De Rosa ◽  
...  
Keyword(s):  
Cell Density ◽  
High Cell Density ◽  
Coli Strain ◽  
High Cell ◽  
High Cell Density Cultivation ◽  
E Coli ◽  
Bioengineered Heparin ◽  
Key Enzyme
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