scholarly journals Characterization of the Au Atomic Contact in a Hydrogen Environment Using Vibration Spectroscopy of a Single Molecular Junction

2009 ◽  
Vol 7 ◽  
pp. 53-56
Author(s):  
Manabu Kiguchi ◽  
Tomoka Nakazumi ◽  
Kunio Hashimoto ◽  
Kei Murakoshi
Keyword(s):  
Molecular Junction ◽  
Hydrogen Environment ◽  
Atomic Contact ◽  
Vibration Spectroscopy

Characterization of hydrogen environment anisotropic thermal etching and application to GaN nanostructure fabrication

2015 ◽  
Vol 54 (4) ◽  
pp. 046501 ◽  
Author(s):  
Ryo Kita ◽  
Ryo Hachiya ◽  
Tomoya Mizutani ◽  
Hiroki Furuhashi ◽  
Akihiko Kikuchi
Keyword(s):  
Nanostructure Fabrication ◽  
Hydrogen Environment ◽  
Thermal Etching

Fabrication and Characterization of Decanedithiol Molecular Junction Using Nanogap Electrodes

2012 ◽  
Vol 51 (7R) ◽  
pp. 075201 ◽  
Author(s):  
Yuuta Takahashi ◽  
Manabu Kiguchi ◽  
Masayo Horikawa ◽  
Yasuhisa Naitoh ◽  
Ken Tsutsui ◽  
...  
Keyword(s):  
Molecular Junction ◽  
Fabrication And Characterization ◽  
Nanogap Electrodes

scholarly journals Characterization of Carbon Nanotube Surfaces by Infrared Vibration Spectroscopy

Hyomen Kagaku ◽  
2007 ◽  
Vol 28 (9) ◽  
pp. 509-512 ◽  
Author(s):  
Akihiro UEDA ◽  
Satoshi YAMADA ◽  
Takahide OYA ◽  
Toshio OGINO
Keyword(s):  
Carbon Nanotube ◽  
Vibration Spectroscopy

scholarly journals Fabrication and conductance characterization of single C60 molecular junction in solutions

2009 ◽  
Vol 477 (1-3) ◽  
pp. 189-193 ◽  
Author(s):  
Yan Guo ◽  
Manabu Kiguchi ◽  
Jianwei Zhao ◽  
Kei Murakoshi
Keyword(s):  
Molecular Junction

Identification of promoter PcadR, in silico characterization of cadmium resistant gene cadR and molecular cloning of promoter PcadR from Pseudomonas aeruginosa BC15

2018 ◽  
Vol 34 (12) ◽  
pp. 819-833 ◽  
Author(s):  
Rajkumar Prabhakaran ◽  
Sebastin Nirmal Rajkumar ◽  
Tharmarajan Ramprasath ◽  
Govindan Sadasivam Selvam
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Molecular Cloning ◽  
Binding Affinity ◽  
In Silico ◽  
Dependent Manner ◽  
Atomic Contact ◽  
In Silico Studies ◽  
Metal Sensing

Cadmium (Cd) remediation in Pseudomonas aeruginosa is achieved through the function of two vital genes, cadA and cadR, that code for P-type ATPase (CadA) and transcription regulatory protein (CadR), respectively. Although numerous studies are available on these metal-sensing and regulatory proteins, the promoter of these genes, metal sensing and binding ability, are poorly understood. The present work is aimed at the characterization of the CadR protein, identification of the P cadR promoter and protein–promoter–metal binding affinity using bioinformatics and to validate the results by cloning the P cadR promoter in Escherichia coli DH5α. The promoter regions and its curvature were identified and analysed using PePPER software (University of Groningen, The Netherland) and the Bendit program (Version: v.1.0), respectively. Using Phyre, the three-dimensional structure of CadR was modelled, and the structure was validated by Ramachandran plots. The DNA-binding domain was present in the N-terminal region of CadR. A dimeric interface was observed in helix-turn-helix and metal ion-binding sites at the C-terminal. Docking studies showed higher affinity of Cd to both CadR (Atomic contact energy = −15.04 kcal/Mol) and P cadR (Atomic contact energy = −40.18 kcal/Mol) when compared to other metal ions. CadR with P cadR showed the highest binding affinity (Atomic contact energy= −250.40 kcal/Mol) when compared with P cadA. In vitro studies using green fluorescent protein tagged with P cadR ( gfp-P cadR) cloned in E. coli-expressed gfp protein in a concentration-dependent manner upon Cd exposure. Based on our in silico studies and in vitro molecular cloning analysis, we conclude that P cadR and CadR are active only in the presence of Cd. The CadR protein has the highest binding affinity with P cadR. As it became apparent that the cadR gene regulates the P cadR activity in the presence of Cd with high specificity, and the cadR and P cadR can be used as a biological tool for development of a microbial biosensor.

Electron Microscopy Characterization of Hexagonal Boron Nitride Nano-Sheets Synthesized in Hydrogen Environment

2012 ◽  
Vol 18 (S2) ◽  
pp. 1550-1551
Author(s):  
M. Ahmadi ◽  
M. Sajjad ◽  
P.X. Feng ◽  
M.J. Guinel
Keyword(s):  
Electron Microscopy ◽  
Boron Nitride ◽  
Hexagonal Boron Nitride ◽  
Hydrogen Environment ◽  
Microscopy Characterization

Extended abstract of a paper presented at Microscopy and Microanalysis 2012 in Phoenix, Arizona, USA, July 29 – August 2, 2012.

Characterization of the Single Molecular Junction

2016 ◽  
pp. 61-85 ◽  
Author(s):  
Manabu Kiguchi ◽  
Shintaro Fujii
Keyword(s):  
Molecular Junction

Fabrication and Characterization of Decanedithiol Molecular Junction Using Nanogap Electrodes

2012 ◽  
Vol 51 ◽  
pp. 075201
Author(s):  
Yuuta Takahashi ◽  
Manabu Kiguchi ◽  
Masayo Horikawa ◽  
Yasuhisa Naitoh ◽  
Ken Tsutsui ◽  
...  
Keyword(s):  
Molecular Junction ◽  
Fabrication And Characterization ◽  
Nanogap Electrodes
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