scholarly journals Impedance Spectroscopy of Ion Channels in Tethered Lipid Bilayers

2005 ◽  
Vol 3 ◽  
pp. 203-206 ◽  
Author(s):  
Samuel Terrettaz ◽  
Horst Vogel
Keyword(s):  
Ion Channels ◽  
Impedance Spectroscopy ◽  
Lipid Bilayers

Investigating the Function of Ion Channels in Tethered Lipid Membranes by Impedance Spectroscopy

MRS Bulletin ◽  
2005 ◽  
Vol 30 (3) ◽  
pp. 207-210 ◽  
Author(s):  
Samuel Terrettaz ◽  
Horst Vogel
Keyword(s):  
Ion Channels ◽  
Impedance Spectroscopy ◽  
Ligand Binding ◽  
Lipid Bilayers ◽  
Lipid Membranes ◽  
Gold Electrodes ◽  
Channel Activity ◽  
Electrophysiological Measurements ◽  
Supported Lipid Membranes ◽  
Specific Ligand

AbstractThe function of biologically important ion channels can be measured in supported lipid membranes by impedance spectroscopy. This approach offers substantial advantages over traditional electrophysiological measurements. In this article, we present an overview of the field, with a special emphasis on the reconstitution of ion channels in lipid bilayers tethered to gold electrodes and the modulation of their channel activity by specific ligand binding.

Improved micro-impedance spectroscopy to determine cell barrier properties

2020 ◽  
Vol 4 (3) ◽  
pp. 150-155 ◽  
Author(s):  
Md. Mehadi Hasan Sohag ◽  
Olivier Nicoud ◽  
Racha Amine ◽  
Abir Khalil-Mgharbel ◽  
Jean-Pierre Alcaraz ◽  
...  
Keyword(s):  
Impedance Spectroscopy ◽  
Epithelial Cells ◽  
Lipid Bilayers ◽  
Cultured Cells ◽  
Cell Model ◽  
Measurement Techniques ◽  
Cell Monolayer ◽  
Microfluidic Channel ◽  
Small Surface ◽  
Resistance Pathway

AbstractThe goal of this study was to determine whether the Tethapod system, which was designed to determine the impedance properties of lipid bilayers, could be used for cell culture in order to utilise micro-impedance spectroscopy to examine further biological applications. To that purpose we have used normal epithelial cells from kidney (RPTEC) and a kidney cancer cell model (786-O). We demonstrate that the Tethapod system is compatible with the culture of 10,000 cells seeded to grow on a small area gold measurement electrode for several days without affecting the cell viability. Furthermore, the range of frequencies for EIS measurements were tuned to examine easily the characteristics of the cell monolayer. We demonstrate significant differences in the paracellular resistance pathway between normal and cancer kidney epithelial cells. Thus, we conclude that this device has advantages for the study of cultured cells that include (i) the configuration of measurement and reference electrodes across a microfluidic channel, and (ii) the small surface area of 6 parallel measurement electrodes (2.1 mm2) integrated in a microfluidic system. These characteristics might improve micro-impedance spectroscopy measurement techniques to provide a simple tool for further studies in the field of the patho-physiology of biological barriers.

scholarly journals Bright Ion Channels and Lipid Bilayers

2013 ◽  
Vol 46 (12) ◽  
pp. 2910-2923 ◽  
Author(s):  
Wiktor SzymaŃski ◽  
Duygu Yilmaz ◽  
ArmaĞan Koçer ◽  
Ben L. Feringa
Keyword(s):  
Ion Channels ◽  
Lipid Bilayers

Reconstitution of Single Potassium Channels from Bovine Gallbladder Epithelium

1998 ◽  
Vol 26 (4) ◽  
pp. 188-199
Author(s):  
E Kyriacou
Keyword(s):  
Ion Channels ◽  
Gall Bladder ◽  
Lipid Bilayers ◽  
Membrane Vesicle ◽  
Basolateral Membrane ◽  
Molecular Transport ◽  
Membrane Vesicles ◽  
Potassium Ion ◽  
Potassium Ion Channels ◽  
Adult Cattle

The study of molecular transport across gall-bladder epithelium may contribute to our understanding of the pathophysiology of gall-bladder disease. The aim of this study was to reconstitute and characterize single potassium ion channels in bovine gall-bladder epithelial mucosa – both apical and basolateral aspects. Standard subcellular fractionation techniques were used to form either apical or basolateral closed-membrane vesicles from the mucosal epithelium of fresh gall bladders from healthy young adult cattle. Vesicular ion channels were incorporated into voltage-clamped planar lipid bilayers under known ionic conditions and their conductances, reversal potentials, and voltages were characterized. Low-conductance voltage-insensitive apical membrane vesicle channels of at least four conductance levels were found (mean ± SD): 12 ± 4 pS, n = 10; 40 ± 12 pS, n = 4; 273 ± 31 pS, n = 3; and 151 ± 24 pS, n = 5. Conductances of potassium ion channels in basolateral membrane vesicles were in the range 9–450 pS, and these channels included high-conductance calcium-activated potassium-ion channels ‘K(Ca)’ which were voltage- and calcium-dependent.

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