Chemiluminescence and Antibody-dependent, Cell-mediated Cytotoxicity between Human Alveolar Macrophages and Peripheral Blood Monocytes in Smokers, Nonsmokers, and Lung Cancer Patients

CHEST Journal ◽  
1989 ◽  
Vol 95 (3) ◽  
pp. 553-557 ◽  
Author(s):  
Ching-Chi Lin ◽  
Wen-Chu Huang ◽  
Ching-Yuang Lin
Keyword(s):  
Lung Cancer ◽  
Cancer Patients ◽  
Alveolar Macrophages ◽  
Peripheral Blood ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Lung Cancer Patients ◽  
Human Alveolar Macrophages ◽  
Dependent Cell

scholarly journals High PD-L1/CD274 Expression of Monocytes and Blood Dendritic Cells Is a Risk Factor in Lung Cancer Patients Undergoing Treatment with PD1 Inhibitor Therapy

Cancers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2966
Author(s):  
Dagmar Riemann ◽  
Wolfgang Schütte ◽  
Steffi Turzer ◽  
Barbara Seliger ◽  
Miriam Möller
Keyword(s):  
Lung Cancer ◽  
Dendritic Cells ◽  
Risk Factor ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Therapy Response ◽  
Inhibitor Therapy ◽  
Strong Positive Correlation ◽  
Blood Monocytes ◽  
Lung Cancer Patients

The aim of this study was to investigate the expression of the coinhibitory molecule PD-L1/CD274 in monocytes and dendritic cells (DC) in the blood of lung cancer patients undergoing PD1 inhibitor therapy and to correlate data with patient’s outcome. PD-L1/CD274 expression of monocytes, CD1c+ myeloid DC (mDC) and CD303+ plasmacytoid DC (pDC) was determined by flow cytometry in peripheral blood at immunotherapy onset. The predictive value of the PD-L1/CD274-expression data was determined by patients’ survival analysis. Patients with a high PD-L1/CD274 expression of monocytes and blood DC subpopulations rarely responded to PD1 inhibitor therapy. Low PD-L1/CD274 expression of monocytes and DC correlated with prolonged progression-free survival (PFS) as well as overall survival (OS). The highest PD-L1/CD274 expression was found in CD14+HLA-DR++CD16+ intermediate monocytes. Whereas the PD-L1/CD274 expression of monocytes and DC showed a strong positive correlation, only the PD-L1/CD274 expression of DC inversely correlated with DC amounts and lymphocyte counts in peripheral blood. Our results implicate that a high PD-L1/CD274 expression of blood monocytes and DC subtypes is a risk factor for therapy response and for the survival of lung cancer patients undergoing PD1 inhibitor therapy.

scholarly journals Proteomic and bioinformatics profile of paired human alveolar macrophages and peripheral blood monocytes

PROTEOMICS ◽  
2015 ◽  
Vol 15 (22) ◽  
pp. 3797-3805 ◽  
Author(s):  
Sara E. Tomechko ◽  
Kathleen C. Lundberg ◽  
Jessica Jarvela ◽  
Gurkan Bebek ◽  
Nicole G. Chesnokov ◽  
...  
Keyword(s):  
Alveolar Macrophages ◽  
Peripheral Blood ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Human Alveolar Macrophages

Effects of Cytokines on Oxygen Radical Production by Peripheral Blood Monocytes and Alveolar Macrophages in Patients with Lung Cancer

1996 ◽  
Vol 82 (4) ◽  
pp. 382-385 ◽  
Author(s):  
Yuji Tohda ◽  
Takashi Iwanaga ◽  
Hisao Uejima ◽  
Yukio Nagasaka ◽  
Shigenori Nakajima
Keyword(s):  
Lung Cancer ◽  
Alveolar Macrophages ◽  
Peripheral Blood ◽  
Interleukin 2 ◽  
Oxygen Radical ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Cancer Group ◽  
Radical Production ◽  
Oxygen Radical Production

The effects of cytokines (interleukin-2, tumor necrosis factor-alpha and interferon-gamma) on the ability of peripheral blood monocytes and alveolar macrophages to produce oxygen radicals were examined by the chemiluminescence assay in patients with lung cancer. Oxygen radical production by peripheral blood monocytes before stimulation with cytokines was lower in the lung cancer group than in healthy controls, suggesting reduced immune function in lung cancer patients. However, the activity in the lung cancer group was elevated to the control level when the monocytes were stimulated by any of the three aforementioned cytokines. Oxygen radical production by alveolar macrophages did not differ significantly between nonstimulated monocytes from lung cancer patients and those from healthy controls. In the lung cancer group, stimulation of the macrophages with any of the three cytokines elevated their ability to produce oxygen radicals to the same extent as in the control group. The results suggest that stimulation of macrophages by interleukin-2, tumor necrosis factor-alpha or interferon-gamma can exert an antitumor action in patients with lung cancer.

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