In Vivo Microscopic Imaging of Frothy Alveolar Aggregates in Pneumocystis Pneumonia Using Fibred Confocal Fluorescence Microscopy

CHEST Journal ◽  
2015 ◽  
Vol 148 (4) ◽  
pp. 100A
Author(s):  
Peng Meng ◽  
Gan Liang Tan ◽  
Su Ying Low ◽  
Angela Takano ◽  
Yuen Li Ng ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Pneumocystis Pneumonia ◽  
Confocal Fluorescence Microscopy ◽  
Microscopic Imaging ◽  
Confocal Fluorescence

Smart imaging of acute lung injury: exploration of myeloperoxidase activity using in vivo endoscopic confocal fluorescence microscopy

2015 ◽  
Vol 309 (6) ◽  
pp. L543-L551 ◽  
Author(s):  
Frédéric Chagnon ◽  
Alexandra Bourgouin ◽  
Réjean Lebel ◽  
Marc-André Bonin ◽  
Eric Marsault ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Lung Injury ◽  
Fluorescence Microscopy ◽  
Real Time ◽  
Ex Vivo ◽  
Polymorphonuclear Neutrophils ◽  
Confocal Fluorescence Microscopy ◽  
Myeloperoxidase Activity ◽  
Confocal Fluorescence

The pathophysiology of acute lung injury (ALI) is well characterized, but its real-time assessment at bedside remains a challenge. When patients do not improve after 1 wk despite supportive therapies, physicians have to consider open lung biopsy (OLB) to identify the process(es) at play. Sustained inflammation and inadequate repair are often observed in this context. OLB is neither easy to perform in a critical setting nor exempt from complications. Herein, we explore intravital endoscopic confocal fluorescence microscopy (ECFM) of the lung in vivo combined with the use of fluorescent smart probe(s) activated by myeloperoxidase (MPO). MPO is a granular enzyme expressed by polymorphonuclear neutrophils (PMNs) and alveolar macrophages (AMs), catalyzing the synthesis of hypoclorous acid, a by-product of hydrogen peroxide. Activation of these probes was first validated in vitro in relevant cells (i.e., AMs and PMNs) and on MPO-non-expressing cells (as negative controls) and then tested in vivo using three rat models of ALI and real-time intravital imaging with ECFM. Semiquantitative image analyses revealed that in vivo probe-related cellular/background fluorescence was associated with corresponding enhanced lung enzymatic activity and was partly prevented by specific MPO inhibition. Additional ex vivo phenotyping was performed, confirming that fluorescent cells were neutrophil elastase+(PMNs) or CD68+(AMs). This work is a first step toward “virtual biopsy” of ALI without OLB.

Detection of Cholangiocarcinoma In Vivo Using Miniprobe-Based Confocal Fluorescence Microscopy

2008 ◽  
Vol 6 (9) ◽  
pp. 1057-1060 ◽  
Author(s):  
Alexander Meining ◽  
Eckart Frimberger ◽  
Valentin Becker ◽  
Stefan Von Delius ◽  
Claus Hann Von Weyhern ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Confocal Fluorescence Microscopy ◽  
Confocal Fluorescence

In vivo assessment of the pulmonary microcirculation in elastase-induced emphysema using probe-based confocal fluorescence microscopy

IntraVital ◽  
2012 ◽  
Vol 1 (2) ◽  
pp. 122-131 ◽  
Author(s):  
Mathieu Salaün ◽  
Romain Modzelewski ◽  
Jean-Paul Marie ◽  
Sophie Moreno-Swirc ◽  
Geneviève Bourg-Heckly ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Confocal Fluorescence Microscopy ◽  
Confocal Fluorescence ◽  
Pulmonary Microcirculation ◽  
In Vivo Assessment

Transgastric in vivo histology in the peritoneal cavity using miniprobe-based confocal fluorescence microscopy in an acute porcine model

Endoscopy ◽  
2007 ◽  
Vol 39 (05) ◽  
pp. 407-411 ◽  
Author(s):  
S. Delius ◽  
H. Feussner ◽  
D. Wilhelm ◽  
A. Karagianni ◽  
J. Henke ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Peritoneal Cavity ◽  
Porcine Model ◽  
Confocal Fluorescence Microscopy ◽  
Confocal Fluorescence
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