The Diagnostic Value of Exhaled Breath Condensate (EBC) in Determination of Genetic Predisposition in Nonsmall Cell Lung Cancer

CHEST Journal ◽  
2013 ◽  
Vol 144 (4) ◽  
pp. 656A
Author(s):  
Mehmet Polatli ◽  
Gokay Bozkurt ◽  
Orhan Cildag
Keyword(s):  
Lung Cancer ◽  
Cell Lung Cancer ◽  
Genetic Predisposition ◽  
Exhaled Breath Condensate ◽  
Nonsmall Cell Lung Cancer ◽  
Diagnostic Value ◽  
Exhaled Breath ◽  
Breath Condensate

Methylation of P16 in exhaled breath condensate for diagnosis of non-small cell lung cancer

Lung Cancer ◽  
2014 ◽  
Vol 83 (1) ◽  
pp. 56-60 ◽  
Author(s):  
Ping Xiao ◽  
Jian-rong Chen ◽  
Feng Zhou ◽  
Chen-xi Lu ◽  
Qichan Yang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Exhaled Breath Condensate ◽  
Small Cell ◽  
Exhaled Breath ◽  
Small Cell Lung ◽  
Breath Condensate

Interleukin-6 is Increased in Breath Condensate of Patients with Non-Small Cell Lung Cancer

2002 ◽  
Vol 17 (2) ◽  
pp. 141-145 ◽  
Author(s):  
G.E. Carpagnano ◽  
O. Resta ◽  
M.P. Foschino-Barbaro ◽  
E. Gramiccioni ◽  
F. Carpagnano
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Exhaled Breath Condensate ◽  
Small Cell ◽  
Exhaled Breath ◽  
Small Cell Lung ◽  
Significant Difference ◽  
Nsclc Patients ◽  
Breath Condensate

Despite recent advances in the diagnosis and treatment of non-small cell lung cancer (NSCLC), most patients still present with advanced stage disease at the time of diagnosis. Recent studies suggest that IL-6 is involved in the development of lung cancer. The aim of the present study was to investigate whether the measurement of IL-6 levels in the breath condensate of NSCLC patients could be used to bring forward the moment of diagnosis and to monitor the progression of the disease. Twenty patients with histological evidence of NSCLC (14 men and 6 women, age 63±8 years) and 15 healthy controls (8 men and 7 women, age 45±6 years) were enrolled in the study. IL6 was measured in the exhaled breath condensate of patients and controls by means of a specific enzyme immunoassay kit. Higher concentrations of exhaled IL-6 were found in NSCLC patients (9.6±0.3 pg/mL) than in controls (3.5±0.2 pg/mL). A statistically significant difference was observed between patients with NSCLC at different stages: higher concentrations of IL-6 (10.9±0.5 pg/mL) were found in patients with metastatic disease than in those with stage III (9.7±0.4 pg/mL), stage II (8.9±0.3 pg/mL) and stage I disease (7.9±0.3 pg/mL). These findings suggest that the measurement of IL-6 in the breath condensate of patients with NSCLC could be proposed as a parameter to take into account in early diagnosis and disease monitoring.

scholarly journals Analysis of p16 gene mutations and their expression using exhaled breath condensate in non-small-cell lung cancer

2015 ◽  
Vol 10 (3) ◽  
pp. 1477-1480 ◽  
Author(s):  
JIN-LIANG CHEN ◽  
JIAN-RONG CHEN ◽  
FEN-FEN HUANG ◽  
GUO-HUA TAO ◽  
FENG ZHOU ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Exhaled Breath Condensate ◽  
Gene Mutations ◽  
Small Cell ◽  
Exhaled Breath ◽  
Small Cell Lung ◽  
P16 Gene ◽  
Breath Condensate

scholarly journals Clinical Value of Serum and Exhaled Breath Condensate miR-186 and IL-1β Levels in Non-Small Cell Lung Cancer

2020 ◽  
Vol 19 ◽  
pp. 153303382094749
Author(s):  
Haiqin Xie ◽  
Jinliang Chen ◽  
Xuedong Lv ◽  
Lu Zhang ◽  
Jinnan Wu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Exhaled Breath Condensate ◽  
Small Cell ◽  
Expression Level ◽  
Exhaled Breath ◽  
Small Cell Lung ◽  
Diagnostic Efficacy ◽  
Breath Condensate

Objective: Our study aimed to investigate the expression level and clinical significance of serum and exhaled breath condensate miR-186 and IL-1β in non-small cell lung cancer patients. Methods: The serum and exhaled breath condensate specimens of 62 non-small cell lung cancer patients and 60 healthy controls were collected to detect miR-186 expression levels by real-time fluorescent quantitative PCR. Enzyme linked immunosorbent assay was applied to examine IL-1β concentration. Statistical analyses were used to evaluate the correlation between miR-186 and IL-1β in serum and clinicopathological features, traditional serum tumor markers, and inflammatory markers. The diagnostic efficacy of miR-186 and IL-1β for non-small cell lung cancer was evaluated by receiver operating characteristic curve analysis. The correlation between miR-186 and IL-1β was determined. Results: ① The relative expression level of miR-186 was greatly reduced in the serum and EBC of patients with non-small cell lung cancer, and the miR-186 expression level was reduced in different TNM stages of non-small cell lung cancer, from the early to later stages. ② The IL-1β concentration in serum and exhaled breath condensate of patients with non-small cell lung cancer was increased. ③ Serum miR-186 and IL-1β levels were closely related to lymph node metastasis, and the low expression of serum miR-186 and the high concentration of IL-1β were associated with higher serum carcinoembryonic antigen, C-reactive protein, and erythrocyte sedimentation rate levels. ④ ROC curve analysis showed that exhaled breath condensate miR-186 had higher area under the curve than serum miR-186, and the combined detection showed higher diagnostic efficacy than the separate detection. In addition, the combined detection of IL-1β and miR-186 has a larger AUC than the separate detection of both. ⑤ The correlation between serum miR-186 and IL-1β was negative. Conclusion: miR-186 and IL-1β are expected to be potential diagnostic biomarkers for non-small cell lung cancer.

scholarly journals Exhaled breath condensate biomarkers for the early diagnosis of lung cancer using proteomics

2017 ◽  
Vol 313 (4) ◽  
pp. L664-L676 ◽  
Author(s):  
Laura M. López-Sánchez ◽  
Bernabé Jurado-Gámez ◽  
Nuria Feu-Collado ◽  
Araceli Valverde ◽  
Amanda Cañas ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Proteomic Analysis ◽  
Exhaled Breath Condensate ◽  
Characteristic Curve ◽  
Area Under The Curve ◽  
Diagnostic Value ◽  
Chronic Obstructive ◽  
Exhaled Breath ◽  
Diagnosis Of Lung Cancer ◽  
Breath Condensate

We explored whether the proteomic analysis of exhaled breath condensate (EBC) may provide biomarkers for noninvasive screening for the early detection of lung cancer (LC). EBC was collected from 192 individuals [49 control (C), 49 risk factor-smoking (S), 46 chronic obstructive pulmonary disease (COPD) and 48 LC]. With the use of liquid chromatography and tandem mass spectrometry, 348 different proteins with a different pattern among the four groups were identified in EBC samples. Significantly more proteins were identified in the EBC from LC compared with other groups (C: 12.4 ± 1.3; S: 15.3 ± 1; COPD: 14 ± 1.6; LC: 24.2 ± 3.6; P = 0.0001). Furthermore, the average number of proteins identified per sample was significantly higher in LC patients, and receiver operating characteristic curve (ROC) analysis showed an area under the curve of 0.8, indicating diagnostic value. Proteins frequently detected in EBC, such as dermcidin and hornerin, along with others much less frequently detected, such as hemoglobin and histones, were identified. Cytokeratins (KRTs) were the most abundant proteins in EBC samples, and levels of KRT6A, KRT6B, and KRT6C isoforms were significantly higher in samples from LC patients ( P = 0.0031, 0.0011, and 0.0009, respectively). Moreover, the amount of most KRTs in EBC samples from LC patients showed a significant positive correlation with tumor size. Finally, we used a random forest algorithm to generate a robust model using EBC protein data for the diagnosis of patients with LC where the area under the ROC curve obtained indicated a good classification (82%). Thus this study demonstrates that the proteomic analysis of EBC samples is an appropriated approach to develop biomarkers for the diagnosis of lung cancer.

3p Microsatellite Alterations in Exhaled Breath Condensate from Patients with Non–Small Cell Lung Cancer

2005 ◽  
Vol 172 (6) ◽  
pp. 738-744 ◽  
Author(s):  
Giovanna E. Carpagnano ◽  
Maria Pia Foschino-Barbaro ◽  
Giuseppina Mulé ◽  
Onofrio Resta ◽  
Stefania Tommasi ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cell Lung Cancer ◽  
Exhaled Breath Condensate ◽  
Small Cell ◽  
Exhaled Breath ◽  
Small Cell Lung ◽  
Breath Condensate

Simultaneous and Sensitive Determination of Amphetamine, Codeine and Morphine in Exhaled Breath Condensate, Using Capillary Electrophoresis Coupled with On-line and Off-line Enhancing Methods

2020 ◽  
Vol 16 (7) ◽  
pp. 872-879
Author(s):  
Samin Hamidi
Keyword(s):  
Drugs Of Abuse ◽  
Exhaled Breath Condensate ◽  
Direct Injection ◽  
Forensic Toxicology ◽  
Analytical Signal ◽  
Exhaled Breath ◽  
Concentration Method ◽  
Validation Parameters ◽  
Breath Condensate

Background: Abuse of drugs is associated with several medical, forensic, toxicology and social challenges. “Drugs of abuse” testing is therefore an important issue. Objective: We propose a simple CE-based method for the quantification of amphetamine, codeine and morphine after direct injection of Exhaled Breath Condensate (EBC) by the aid of simple stacking mode and an off-line pre-concentration method. Methods: Using graphene oxide adsorbents, amphetamine, codeine and morphine were extracted from EBC in order to eliminate the proteins and other interferences. In addition to off-line method, an online stacking mode was applied to improve the analytical signal obtained from the instrument. Results: The validation parameters were experimented on the developed method based on the FDA guideline over concentration ranges of 12.5-100, 30-500 and 10-1250 ng/mL associated with amphetamine, codeine and morphine, respectively. Small volumes (around 100 μL) of EBC were collected using a lab-made setup and successfully analyzed using the proposed method where precisions and accuracies (within day and between days) were in accordance with the guideline (recommended less than 15 % for biological samples). The recovery tests were used to evaluate the matrix effect and data (94 to 105 %) showed that the proposed method can be applied in different EBC matrix samplings of subjects. Conclusion: The proposed method is superior for simultaneous determination of amphetamine, codeine and morphine over chromatographic analyses because it is fast and consumes fewer chemicals, with no derivatization step.

Sign in / Sign up

Export Citation Format

Share Document