Real-time PCR in Bronchoalveolar Lavage Fluid in the Sputum Smear-Negative Patients Suspected of Pulmonary Tuberculosis by Chest CT

CHEST Journal ◽  
2010 ◽  
Vol 138 (4) ◽  
pp. 673A
Author(s):  
Yee Hyung Kim ◽  
Sohee Park ◽  
Cheon Woong Choi ◽  
Jee-Hong Yoo ◽  
Hong Mo Kang ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Bronchoalveolar Lavage ◽  
Real Time ◽  
Real Time Pcr ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Chest Ct ◽  
Sputum Smear ◽  
Smear Negative

scholarly journals Inter-laboratory comparison of three different real-time PCR assays for the detection of Pneumocystis jiroveci in bronchoalveolar lavage fluid samples

2006 ◽  
Vol 55 (9) ◽  
pp. 1229-1235 ◽  
Author(s):  
Catharina F. M. Linssen ◽  
Jan A. Jacobs ◽  
Pieter Beckers ◽  
Kate E. Templeton ◽  
Judith Bakkers ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Real Time ◽  
Real Time Pcr ◽  
Bronchoalveolar Lavage Fluid ◽  
Medical Center ◽  
Tertiary Care ◽  
Lavage Fluid ◽  
The Other ◽  
Pneumocystis Jiroveci ◽  
Pcr Assays

Pneumocystis jiroveci pneumonia (PCP) is an opportunistic infection affecting immunocompromised patients. While conventional diagnosis of PCP by microscopy is cumbersome, the use of PCR to diagnose PCP has great potential. Nevertheless, inter-laboratory validation and standardization of PCR assays is lacking. The aim of this study was to evaluate the inter-laboratory agreement of three independently developed real-time PCR assays for the detection of P. jiroveci in bronchoalveolar lavage fluid samples. Therefore, 124 samples were collected in three tertiary care laboratories (Leiden University Medical Center, Maastricht Infection Center and Radboud University Nijmegen Medical Centre) and were tested by both microscopy and real-time PCR. Of 41 samples positive for P. jiroveci by microscopy, 40 were positive in all three PCR assays. The remaining sample was positive in a single assay only. Out of 83 microscopy-negative samples, 69 were negative in all three PCR assays. The other 14 samples were found positive, either in all three assays (n=5), in two (n=2) or in one of the assays (n=7). The data demonstrate high inter-laboratory agreement among real-time PCR assays for the detection of P. jiroveci.

scholarly journals Role of bronchoalveolar lavage fluid and serum interleukin-27 in the diagnosis of smear-negative pulmonary tuberculosis

Medicine ◽  
2021 ◽  
Vol 100 (20) ◽  
pp. e25821
Author(s):  
Feng Zhu ◽  
Qinfang Ou ◽  
Jian Zheng ◽  
Min Zhou ◽  
Huaxin Chen ◽  
...  
Keyword(s):  
Pulmonary Tuberculosis ◽  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Interleukin 27 ◽  
Smear Negative
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