Clinical Efficacy of Direct DNA Sequencing Analysis on Sputum Specimens for Early Detection of Drug-Resistant Mycobacterium tuberculosis in a Clinical Setting

CHEST Journal ◽  
2010 ◽  
Vol 137 (2) ◽  
pp. 393-400 ◽  
Author(s):  
Jeong-Hee Choi ◽  
Kyung Wha Lee ◽  
Hye-Ryun Kang ◽  
Yong Il Hwang ◽  
SeungHun Jang ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Early Detection ◽  
Dna Sequencing ◽  
Clinical Efficacy ◽  
Clinical Setting ◽  
Sequencing Analysis ◽  
Drug Resistant ◽  
Direct Dna Sequencing ◽  
Dna Sequencing Analysis

scholarly journals Butyrylcholinesterase (BCHE) Genotyping for Post-Succinylcholine Apnea in an Australian Population

2003 ◽  
Vol 49 (8) ◽  
pp. 1297-1308 ◽  
Author(s):  
Tina Yen ◽  
Brian N Nightingale ◽  
Jennifer C Burns ◽  
David R Sullivan ◽  
Peter M Stewart
Keyword(s):  
Dna Sequencing ◽  
Gene Mutations ◽  
University Teaching ◽  
Sequencing Analysis ◽  
Direct Dna Sequencing ◽  
Bche Activity ◽  
Mutation Screen ◽  
Sequencing Studies ◽  
Dna Sequencing Analysis ◽  
K Variant

Abstract Background: Measurement of plasma butyrylcholinesterase (BChE) activity and inhibitor-based phenotyping are standard methods for identifying patients who experience post-succinylcholine (SC) apnea attributable to inherited variants of the BChE enzyme. Our aim was to develop PCR-based assays for BCHE mutation detection and implement them for routine diagnostic use at a university teaching hospital. Methods: Between 1999 and 2002, we genotyped 65 patients referred after prolonged post-SC apnea. Five BCHE gene mutations were analyzed. Competitive oligo-priming (COP)-PCR was used for flu-1, flu-2, and K-variant and direct DNA sequencing analysis for dibucaine and sil-1 mutations. Additional DNA sequencing of BCHE coding regions was provided when the five-mutation screen was negative or mutation findings were inconsistent with enzyme activity. Results: Genotyping identified 52 patients with primary hypocholinesterasemia attributable to BCHE mutations, and in 44 individuals the abnormalities were detected by the five-mutation screen (detection rate, 85%). Additional sequencing studies revealed mutations in eight other patients, including five with novel mutations. The most common genotype abnormality was compound homozygous dibucaine and homozygous K-variant mutations. No simple homozygotes were found. Of the remaining 13 patients, 3 had normal BChE activity and gene, and 10 were diagnosed with hypocholinesterasemia unrelated to BCHE gene abnormalities. Conclusion: A five-mutation screen for investigation of post-SC apnea identified BCHE gene abnormalities for 80% of a referral population. Six new BCHE mutations were identified by sequencing studies of 16 additional patients.

Human c-fms proto-oncogene: comparative analysis with an abnormal allele

1985 ◽  
Vol 5 (2) ◽  
pp. 422-426
Author(s):  
J S Verbeek ◽  
A J Roebroek ◽  
A M van den Ouweland ◽  
H P Bloemers ◽  
W J Van de Ven
Keyword(s):  
Comparative Analysis ◽  
Dna Sequencing ◽  
Sequencing Analysis ◽  
Base Pairs ◽  
Small Deletion ◽  
Viral Oncogene ◽  
Close Proximity ◽  
Genetic Sequences ◽  
Dna Sequencing Analysis

The organization of the human c-fms proto-oncogene has been determined and compared with an abnormal allele. The human v-fms homologous genetic sequences are dispersed discontinuously and colinearly with the viral oncogene over a DNA region of ca. 32 kilobase pairs. The abnormal c-fms locus contains a small deletion in its 3' portion. DNA sequencing analysis indicated that it was 426 base pairs in size and located in close proximity to a putative c-fms exon.

scholarly journals Comparison of Angelica Species Roots Using Taste Sensor and DNA Sequencing Analysis

2012 ◽  
Vol 27 (6) ◽  
pp. 37-42 ◽  
Author(s):  
Young Hwa Kim ◽  
Goya Choi ◽  
Hye Won Lee ◽  
Gwan Ho Lee ◽  
Seong Wook Chae ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Sequencing Analysis ◽  
Dna Sequencing Analysis ◽  
Taste Sensor

Comparative View of In Silico DNA Sequencing Analysis Tools

2011 ◽  
pp. 207-221 ◽  
Author(s):  
Sissades Tongsima ◽  
Anunchai Assawamakin ◽  
Jittima Piriyapongsa ◽  
Philip J. Shaw
Keyword(s):  
Dna Sequencing ◽  
In Silico ◽  
Sequencing Analysis ◽  
Analysis Tools ◽  
Dna Sequencing Analysis

CALMETTE-GU??RIN BACILLUS STERNAL OSTEOMYELITIS DIAGNOSED BY DNA SEQUENCING ANALYSIS OF PNC A

2004 ◽  
Vol 23 (8) ◽  
pp. 784-786 ◽  
Author(s):  
Wei-Jen Lin ◽  
Jang-Jih Lu ◽  
Chih-Chun Chu ◽  
Tein-Yao Chang ◽  
Chih-Chien Wang
Keyword(s):  
Dna Sequencing ◽  
Sequencing Analysis ◽  
Sternal Osteomyelitis ◽  
Dna Sequencing Analysis
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