scholarly journals Different functional states of fusion protein gB revealed on human cytomegalovirus by cryo electron tomography with Volta phase plate

2018 ◽  
Vol 14 (12) ◽  
pp. e1007452 ◽  
Author(s):  
Zhu Si ◽  
Jiayan Zhang ◽  
Sakar Shivakoti ◽  
Ivo Atanasov ◽  
Chang-Lu Tao ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Human Cytomegalovirus ◽  
Electron Tomography ◽  
Phase Plate ◽  
Cryo Electron Tomography ◽  
Functional States

In situ Structure of Viral RNA by Cryo Electron Tomography with Volta Phase Plate, Energy Filtering and Direct Electron Counting

2016 ◽  
Vol 22 (S3) ◽  
pp. 74-75
Author(s):  
Z. Hong Zhou ◽  
Wong H. Hui ◽  
Jiayan Zhang ◽  
Ivo Atanasov ◽  
Cristina C. Celma ◽  
...  
Keyword(s):  
Electron Tomography ◽  
Viral Rna ◽  
Phase Plate ◽  
Energy Filtering ◽  
Electron Counting ◽  
Cryo Electron Tomography

scholarly journals Phase Contrast Cryo-Electron Tomography and Single Particle Analysis with a New Phase Plate

2014 ◽  
Vol 20 (S3) ◽  
pp. 232-233
Author(s):  
Maryam Khoshouei ◽  
Radostin Danev ◽  
Günther Gerisch ◽  
Maria Ecke ◽  
Juergen Plitzko ◽  
...  
Keyword(s):  
Single Particle ◽  
Phase Contrast ◽  
Electron Tomography ◽  
Particle Analysis ◽  
Phase Plate ◽  
Single Particle Analysis ◽  
Cryo Electron Tomography ◽  
New Phase

scholarly journals Structural Analysis of Hemagglutinin-Induced Hemifusion by Volta Phase-Plate Cryo-Electron Tomography

2016 ◽  
Vol 110 (3) ◽  
pp. 250a-251a
Author(s):  
Petr Chlanda ◽  
Elena Mekhedov ◽  
Hang Waters ◽  
Cindi L. Schwartz ◽  
Elizabeth R. Fischer ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Electron Tomography ◽  
Phase Plate ◽  
Cryo Electron Tomography

scholarly journals Requirements for Phase-Plate Cryo-Electron Tomography

2010 ◽  
Vol 16 (S2) ◽  
pp. 546-547
Author(s):  
M Marko ◽  
C Hsieh ◽  
A Leith ◽  
C Mannella
Keyword(s):  
Electron Tomography ◽  
Phase Plate ◽  
Cryo Electron Tomography

Extended abstract of a paper presented at Microscopy and Microanalysis 2010 in Portland, Oregon, USA, August 1 – August 5, 2010.

scholarly journals Artifact Correction for Zernike Phase-Plate Cryo-Electron Tomography

2014 ◽  
Vol 20 (S3) ◽  
pp. 234-235 ◽  
Author(s):  
Haixin Sui ◽  
Gregory Kishchenko ◽  
Radostin Danev ◽  
Jie He ◽  
Rebecca Fisher ◽  
...  
Keyword(s):  
Electron Tomography ◽  
Phase Plate ◽  
Artifact Correction ◽  
Cryo Electron Tomography

scholarly journals Dual-axis Volta phase plate cryo-electron tomography of Ebola virus-like particles reveals actin-VP40 interactions

2021 ◽  
Author(s):  
Sophie L. Winter ◽  
Petr Chlanda
Keyword(s):  
Actin Filaments ◽  
Ebola Virus ◽  
Electron Tomography ◽  
Direct Interaction ◽  
Viral Envelope ◽  
Phase Plate ◽  
High Contrast ◽  
Slab Geometry ◽  
Virus Like Particles ◽  
Cryo Electron Tomography

AbstractCryo-electron tomography (cryo-ET) is a pivotal imaging technique for studying the structure of pleomorphic enveloped viruses and their interactions with the host at native conditions. Owing to the limited tilting range of samples with a slab geometry, electron tomograms suffer from so-called missing wedge information in Fourier space. In dual-axis cryo-ET, two tomograms reconstructed from orthogonally oriented tilt series are combined into a tomogram with improved resolution as the missing wedge information is reduced to a pyramid. Volta phase plate (VPP) allows to perform in-focus cryo-ET with high contrast transfer at low-resolution frequencies and thus its application may improve the quality of dual-axis tomograms. Here, we compare dual-axis cryo-ET with and without VPP on Ebola virus-like particles to visualize and segment viral and host cell proteins within the membrane-enveloped filamentous particles. Dual-axis VPP cryo-ET reduces the missing wedge information and ray artifacts arising from the weighted back-projection during tomogram reconstruction, thereby minimizing ambiguity in the analysis of crowded environments and facilitating 3D segmentation. We show that dual-axis VPP tomograms provide a comprehensive description of macromolecular organizations such as nucleocapsid assembly states, the distribution of glycoproteins on the viral envelope and asymmetric arrangements of the VP40 layer in non-filamentous regions of virus-like particles. Our data reveal actin filaments within virus-like particles in close proximity to the viral VP40 scaffold, suggesting a direct interaction between VP40 and actin filaments. Dual-axis VPP cryo-ET provides more complete 3D information at high contrast and allows for better interpretation of macromolecule interactions and pleomorphic organizations.HighlightsVolta phase plate dual-axis cryo-electron tomography provides high contrast tomography data with reduced back-projection ray artifacts and missing wedge information in Fourier spaceVolta phase plate dual-axis cryo-electron tomography facilitates interpretation of protein-membrane interactionsVolta phase plate dual-axis cryo-electron tomography reduces ambiguity in manual 3D rendering and markedly improves 3D isosurface modelingEbola virus-like particles contain actin filaments in close proximity to the VP40 layer

scholarly journals Exploring Cellular Morphology of Thermoplasma acidophilum by Cryo-Electron Tomography with Volta Phase Plate

2017 ◽  
Vol 23 (S1) ◽  
pp. 1234-1235
Author(s):  
Yoshiyuki Fukuda ◽  
Florian Beck ◽  
Istvan Nagy ◽  
Radostin Danev ◽  
Wolfgang Baumeister
Keyword(s):  
Electron Tomography ◽  
Cellular Morphology ◽  
Phase Plate ◽  
Thermoplasma Acidophilum ◽  
Cryo Electron Tomography
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