scholarly journals Combined single-cell quantitation of host and SIV genes and proteins ex vivo reveals host-pathogen interactions in individual cells

2017 ◽  
Vol 13 (6) ◽  
pp. e1006445 ◽  
Author(s):  
Diane L. Bolton ◽  
Kathleen McGinnis ◽  
Greg Finak ◽  
Pratip Chattopadhyay ◽  
Raphael Gottardo ◽  
...  
Keyword(s):  
Single Cell ◽  
Ex Vivo ◽  
Host Pathogen Interactions ◽  
Host Pathogen

scholarly journals mSphere of Influence: Clearing a Path for High-Resolution Visualization of Host-Pathogen Interactions In Vivo

mSphere ◽  
2019 ◽  
Vol 4 (4) ◽  
Author(s):  
Shumin Tan
Keyword(s):  
Single Cell ◽  
Dimensional Space ◽  
Whole Body ◽  
Host Pathogen Interactions ◽  
Content Type ◽  
3 Dimensional ◽  
Host Interactions ◽  
Host Pathogen ◽  
Tissue Optical Clearing

ABSTRACT Shumin Tan works in the field of Mycobacterium tuberculosis-host interactions. In this mSphere of Influence article, she reflects on how the paper “Single-cell phenotyping within transparent intact tissue through whole-body clearing” by B. Yang et al. (Cell 158:945–958, 2014, https://doi.org/10.1016/j.cell.2014.07.017) impacted her ideas on approaches to visualize and understand heterogeneous host-pathogen interactions in vivo in 3-dimensional space at the single-cell level, through the tractable and broadly compatible tissue optical clearing methods developed.

scholarly journals Precision cut lung slices: a novel versatile tool to examine host–pathogen interaction in the chicken lung

2020 ◽  
Vol 51 (1) ◽  
Author(s):  
Karen Jane Bryson ◽  
Damien Garrido ◽  
Marco Esposito ◽  
Gerry McLachlan ◽  
Paul Digard ◽  
...  
Keyword(s):  
Inflammatory Responses ◽  
Ex Vivo ◽  
Mammalian Species ◽  
Influenza Viruses ◽  
Mononuclear Phagocytes ◽  
Suitable Model ◽  
Type I ◽  
Host Pathogen Interactions ◽  
Host Pathogen ◽  
Precision Cut Lung Slices

AbstractThe avian respiratory tract is a common entry route for many pathogens and an important delivery route for vaccination in the poultry industry. Immune responses in the avian lung have mostly been studied in vivo due to the lack of robust, relevant in vitro and ex vivo models mimicking the microenvironment. Precision-cut lung slices (PCLS) have the major advantages of maintaining the 3-dimensional architecture of the lung and includes heterogeneous cell populations. PCLS have been obtained from a number of mammalian species and from chicken embryos. However, as the embryonic lung is physiologically undifferentiated and immunologically immature, it is less suitable to examine complex host–pathogen interactions including antimicrobial responses. Here we prepared PCLS from immunologically mature chicken lungs, tested different culture conditions, and found that serum supplementation has a detrimental effect on the quality of PCLS. Viable cells in PCLS remained present for ≥ 40 days, as determined by viability assays and sustained motility of fluorescent mononuclear phagocytic cells. The PCLS were responsive to lipopolysaccharide stimulation, which induced the release of nitric oxide, IL-1β, type I interferons and IL-10. Mononuclear phagocytes within the tissue maintained phagocytic activity, with live cell imaging capturing interactions with latex beads and an avian pathogenic Escherichia coli strain. Finally, the PCLS were also shown to be permissive to infection with low pathogenic avian influenza viruses. Taken together, immunologically mature chicken PCLS provide a suitable model to simulate live organ responsiveness and cell dynamics, which can be readily exploited to examine host–pathogen interactions and inflammatory responses.

scholarly journals Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestock

2021 ◽  
Author(s):  
Stephen Meek ◽  
Tom Watson ◽  
Lel Eory ◽  
Gus McFarlane ◽  
Felicity J. Wynne ◽  
...  
Keyword(s):  
Stem Cells ◽  
Infectious Diseases ◽  
Molecular Basis ◽  
Lentiviral Vector ◽  
Ex Vivo ◽  
African Swine Fever Virus ◽  
Respiratory Syndrome Virus ◽  
Host Pathogen Interactions ◽  
Host Pathogen

AbstractInfectious diseases of farmed and wild animals pose a recurrent threat to food security and human health. The macrophage, a key component of the innate immune system, is the first line of defence against many infectious agents and plays a major role in shaping the adaptive immune response. However, this phagocyte is a target and host for many pathogens. Understanding the molecular basis of interactions between macrophages and pathogens is therefore crucial for the development of effective strategies to combat important infectious diseases. We explored how pluripotent stem cells (PSCs) can provide a limitless in vitro supply of genetically and experimentally tractable macrophages from livestock. Porcine and bovine PSC-derived macrophages (PSCdMs) exhibited molecular and functional characteristics of ex vivo primary macrophages. Pig PSCdMs were productively infected by Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) and African Swine Fever Virus (ASFV), two of the most economically important and devastating viruses in pig farming. Moreover, Pig PSCdMs were readily amenable to genetic modification by CRISPR/Cas9 gene editing applied in parental stem cells, or directly by lentiviral vector transduction. PSCs and differentiated derivatives therefore provide a useful and ethical experimental platform to investigate the genetic and molecular basis of host-pathogen interactions in livestock.

scholarly journals Stem cell-derived porcine macrophages as a new platform for studying host-pathogen interactions

BMC Biology ◽  
2022 ◽  
Vol 20 (1) ◽  
Author(s):  
Stephen Meek ◽  
Tom Watson ◽  
Lel Eory ◽  
Gus McFarlane ◽  
Felicity J. Wynne ◽  
...  
Keyword(s):  
Stem Cells ◽  
Infectious Diseases ◽  
Molecular Basis ◽  
Lentiviral Vector ◽  
Ex Vivo ◽  
African Swine Fever Virus ◽  
Respiratory Syndrome Virus ◽  
Host Pathogen Interactions ◽  
Host Pathogen

Abstract Background Infectious diseases of farmed and wild animals pose a recurrent threat to food security and human health. The macrophage, a key component of the innate immune system, is the first line of defence against many infectious agents and plays a major role in shaping the adaptive immune response. However, this phagocyte is a target and host for many pathogens. Understanding the molecular basis of interactions between macrophages and pathogens is therefore crucial for the development of effective strategies to combat important infectious diseases. Results We explored how porcine pluripotent stem cells (PSCs) can provide a limitless in vitro supply of genetically and experimentally tractable macrophages. Porcine PSC-derived macrophages (PSCdMs) exhibited molecular and functional characteristics of ex vivo primary macrophages and were productively infected by pig pathogens, including porcine reproductive and respiratory syndrome virus (PRRSV) and African swine fever virus (ASFV), two of the most economically important and devastating viruses in pig farming. Moreover, porcine PSCdMs were readily amenable to genetic modification by CRISPR/Cas9 gene editing applied either in parental stem cells or directly in the macrophages by lentiviral vector transduction. Conclusions We show that porcine PSCdMs exhibit key macrophage characteristics, including infection by a range of commercially relevant pig pathogens. In addition, genetic engineering of PSCs and PSCdMs affords new opportunities for functional analysis of macrophage biology in an important livestock species. PSCs and differentiated derivatives should therefore represent a useful and ethical experimental platform to investigate the genetic and molecular basis of host-pathogen interactions in pigs, and also have wider applications in livestock.

Spatiotemporal Structure of Host-Pathogen Interactions in a Metapopulation

2009 ◽  
Vol 174 (3) ◽  
pp. 308
Author(s):  
Soubeyrand ◽  
Laine ◽  
Hanski ◽  
Penttinen
Keyword(s):  
Host Pathogen Interactions ◽  
Spatiotemporal Structure ◽  
Host Pathogen
Sign in / Sign up

Export Citation Format

Share Document