scholarly journals Screen of Non-annotated Small Secreted Proteins of Pseudomonas syringae Reveals a Virulence Factor That Inhibits Tomato Immune Proteases

2016 ◽  
Vol 12 (9) ◽  
pp. e1005874 ◽  
Author(s):  
Takayuki Shindo ◽  
Farnusch Kaschani ◽  
Fan Yang ◽  
Judit Kovács ◽  
Fang Tian ◽  
...  
Keyword(s):  
Pseudomonas Syringae ◽  
Virulence Factor ◽  
Secreted Proteins ◽  
Small Secreted Proteins

Two small secreted proteins fromPuccinia triticinainduce reduction of ß-glucoronidase transient expression in wheat isolines containingLr9, Lr24andLr26

2016 ◽  
Vol 38 (1) ◽  
pp. 91-102 ◽  
Author(s):  
Vanesa Segovia ◽  
Myron Bruce ◽  
Jessica L. Shoup Rupp ◽  
Li Huang ◽  
Guus Bakkeren ◽  
...  
Keyword(s):  
Transient Expression ◽  
Secreted Proteins ◽  
Small Secreted Proteins

scholarly journals Absence of repellents in Ustilago maydis induces genes encoding small secreted proteins

2011 ◽  
Vol 100 (2) ◽  
pp. 219-229 ◽  
Author(s):  
Wieke R. Teertstra ◽  
Pauline Krijgsheld ◽  
Han A. B. Wösten
Keyword(s):  
Ustilago Maydis ◽  
Secreted Proteins ◽  
Genes Encoding ◽  
Small Secreted Proteins

scholarly journals Pseudomonas syringae Virulence Factor Syringolin A Counteracts Stomatal Immunity by Proteasome Inhibition

2010 ◽  
Vol 23 (10) ◽  
pp. 1287-1293 ◽  
Author(s):  
Barbara Schellenberg ◽  
Christina Ramel ◽  
Robert Dudler
Keyword(s):  
Pseudomonas Syringae ◽  
Virulence Factor ◽  
Proteasome Inhibitor ◽  
Cell Function ◽  
Stomatal Closure ◽  
Proteasome Inhibition ◽  
Phytopathogenic Bacterium ◽  
Disease Symptoms ◽  
Exogenous Addition ◽  
Peptide Derivative

The peptide derivative syringolin A, a product of a mixed nonribosomal peptide and polyketide synthetase, is secreted by certain strains of the phytopathogenic bacterium Pseudomonas syringae pv. syringae. Syringolin A was shown to be a virulence factor for P. syringae pv. syringae B728a because disease symptoms on its host Phaseolus vulgaris (bean) were greatly reduced upon inoculation with syringolin A-negative mutants. Syringolin A's mode of action was recently shown to be irreversible proteasome inhibition. Here, we report that syringolin A-producing bacteria are able to open stomata and, thus, counteract stomatal innate immunity in bean and Arabidopsis. Syringolin A-negative mutants, which induce stomatal closure, can be complemented by exogenous addition of not only syringolin A but also MG132, a well-characterized and structurally unrelated proteasome inhibitor. This demonstrates that proteasome activity is crucial for guard cell function. In Arabidopsis, stomatal immunity was salicylic acid (SA)-dependent and required NPR1, a key regulator of the SA-dependent defense pathway whose proteasome-dependent turnover has been reported to be essential for its function. Thus, elimination of NPR1 turnover through proteasome inhibition by syringolin A is an attractive hypothesis to explain the observed inhibition of stomatal immunity by syringolin A.

scholarly journals Use of a Secretion Trap Screen in Pepper Following Phytophthora capsici Infection Reveals Novel Functions of Secreted Plant Proteins in Modulating Cell Death

2011 ◽  
Vol 24 (6) ◽  
pp. 671-684 ◽  
Author(s):  
Seon-In Yeom ◽  
Hyang-Ku Baek ◽  
Sang-Keun Oh ◽  
Won-Hee Kang ◽  
Sang Jik Lee ◽  
...  
Keyword(s):  
Cell Death ◽  
Capsicum Annuum ◽  
Pseudomonas Syringae ◽  
Signal Sequence ◽  
Phytophthora Capsici ◽  
Tobacco Rattle Virus ◽  
Secreted Proteins ◽  
In Planta ◽  
Cell Wall Modification ◽  
Cas Genes

In plants, the primary defense against pathogens is mostly inducible and associated with cell wall modification and defense-related gene expression, including many secreted proteins. To study the role of secreted proteins, a yeast-based signal-sequence trap screening was conducted with the RNA from Phytophthora capsici-inoculated root of Capsicum annuum ‘Criollo de Morelos 334’ (CM334). In total, 101 Capsicum annuum secretome (CaS) clones were isolated and identified, of which 92 were predicted to have a secretory signal sequence at their N-terminus. To identify differences in expressed CaS genes between resistant and susceptible cultivars of pepper, reverse Northern blots and real-time reverse-transcription polymerase chain reaction were performed with RNA samples isolated at different time points following P. capsici inoculation. In an attempt to assign biological functions to CaS genes, we performed in planta knock-down assays using the Tobacco rattle virus-based gene-silencing method. Silencing of eight CaS genes in pepper resulted in suppression of the cell death induced by the non-host bacterial pathogen (Pseudomonas syringae pv. tomato T1). Three CaS genes induced phenotypic abnormalities in silenced plants and one, CaS259 (PR4-l), caused both cell death suppression and perturbed phenotypes. These results provide evidence that the CaS genes may play important roles in pathogen defense as well as developmental processes.

Genes encoding a group of related small secreted proteins from the gut of Hessian fly larvae [Mayetiola destructor (Say)]

2006 ◽  
Vol 13 (5) ◽  
pp. 339-348 ◽  
Author(s):  
MING-SHUN CHEN ◽  
XIANG LIU ◽  
YU-CHENG ZHU ◽  
JOHN C. REESE ◽  
GERALD E. WILDE
Keyword(s):  
Hessian Fly ◽  
Mayetiola Destructor ◽  
Secreted Proteins ◽  
Genes Encoding ◽  
Fly Larvae ◽  
Small Secreted Proteins

scholarly journals In-depth secretome analysis of Puccinia striiformis f. sp. tritici in infected wheat uncovers effector functions

2020 ◽  
Author(s):  
Ahmet Caglar Ozketen ◽  
Ayse Andac-Ozketen ◽  
Bayantes Dagvadorj ◽  
Burak Demiralay ◽  
Mahinur S. Akkaya
Keyword(s):  
De Novo ◽  
Puccinia Striiformis ◽  
Yellow Rust ◽  
Expression System ◽  
Differentially Expressed ◽  
Secreted Proteins ◽  
Central Anatolia ◽  
Human Populations ◽  
Post Inoculation ◽  
Small Secreted Proteins

ABSTRACTThe importance of wheat yellow rust disease, caused by Puccinia striiformis f. sp. tritici (Pst), has increased substantially due to the emergence of aggressive new Pst races in the last couple of decades. In an era of escalating human populations and climate change, it is vital to understand the infection mechanism of Pst in order to develop better strategies to combat wheat yellow disease. This study focuses on the identification of small secreted proteins (SSPs) and candidate-secreted effector proteins (CSEPs) that are used by the pathogen to support infection and control disease development. We generated de novo assembled transcriptomes of Pst collected from wheat fields in central Anatolia. We inoculated both susceptible and resistant seedlings with Pst and analyzed haustoria formation. At 10 days post-inoculation (dpi), we analyzed the transcriptomes and identified 10,550 Differentially Expressed Unigenes (DEGs), of which 6,220 were Pst-related. Among those Pst-related genes, 230 were predicted as PstSSPs. In silico characterization was performed using an approach combining the transcriptomic data and data mining results to provide a reliable list to narrow down the ever-expanding repertoire of predicted effectorome. The comprehensive analysis detected 14 Differentially Expressed Small-Secreted Proteins (DESSPs) that overlapped with the genes in available literature data to serve as the best CSEPs for experimental validation. One of the CSEPs was cloned and studied to test the reliability of the presented data. Biological assays show that the randomly selected CSEP, Unigene17495 (PSTG_10917), localizes in the chloroplast and is able to suppress cell death induced by INF1 in a Nicotiana benthamiana heterologous expression system.

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