scholarly journals Protein Kinase R Degradation Is Essential for Rift Valley Fever Virus Infection and Is Regulated by SKP1-CUL1-F-box (SCF)FBXW11-NSs E3 Ligase

2016 ◽  
Vol 12 (2) ◽  
pp. e1005437 ◽  
Author(s):  
Rajini Mudhasani ◽  
Julie P. Tran ◽  
Cary Retterer ◽  
Krishna P. Kota ◽  
Chris A. Whitehouse ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Virus Infection ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
E3 Ligase ◽  
Fever Virus ◽  
Protein Kinase R ◽  
Valley Fever

scholarly journals Human Biomarkers of Outcome Following Rift Valley Fever Virus Infection

2018 ◽  
Vol 218 (11) ◽  
pp. 1847-1851 ◽  
Author(s):  
Anita K McElroy ◽  
Jessica R Harmon ◽  
Timothy Flietstra ◽  
Stuart T Nichol ◽  
Christina F Spiropoulou
Keyword(s):  
Virus Infection ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever

Serological evidence of Rift Valley fever virus infection in slaughtered ruminants in Nigeria

2019 ◽  
Vol 40 (4) ◽  
pp. 367-377 ◽  
Author(s):  
Adewale Victor Opayele ◽  
Linda Amarachi Ndiana ◽  
Georgina Njideka Odaibo ◽  
David Olufemi Olaleye
Keyword(s):  
Virus Infection ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Serological Evidence ◽  
Valley Fever

Protection against Rift Valley fever virus infection in mice upon administration of interferon-inducing RNA transcripts from the FMDV genome

2014 ◽  
Vol 109 ◽  
pp. 64-67 ◽  
Author(s):  
Gema Lorenzo ◽  
Miguel Rodríguez-Pulido ◽  
Elena López-Gil ◽  
Francisco Sobrino ◽  
Belén Borrego ◽  
...  
Keyword(s):  
Virus Infection ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever ◽  
Rna Transcripts

scholarly journals Wolbachia effects on Rift Valley fever virus infection in Culex tarsalis mosquitoes

2017 ◽  
Vol 11 (10) ◽  
pp. e0006050 ◽  
Author(s):  
Brittany L. Dodson ◽  
Elizabeth S. Andrews ◽  
Michael J. Turell ◽  
Jason L. Rasgon
Keyword(s):  
Virus Infection ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Culex Tarsalis ◽  
Valley Fever

scholarly journals Rift Valley Fever Virus NSs Protein Promotes Post-Transcriptional Downregulation of Protein Kinase PKR and Inhibits eIF2α Phosphorylation

2009 ◽  
Vol 5 (2) ◽  
pp. e1000287 ◽  
Author(s):  
Tetsuro Ikegami ◽  
Krishna Narayanan ◽  
Sungyong Won ◽  
Wataru Kamitani ◽  
C. J. Peters ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever ◽  
Eif2α Phosphorylation

scholarly journals NSs Virulence Factor of Rift Valley Fever Virus Engages the F-Box Proteins FBXW11 and β-TRCP1 To Degrade the Antiviral Protein Kinase PKR

2016 ◽  
Vol 90 (13) ◽  
pp. 6140-6147 ◽  
Author(s):  
Markus Kainulainen ◽  
Simone Lau ◽  
Charles E. Samuel ◽  
Veit Hornung ◽  
Friedemann Weber
Keyword(s):  
Protein Kinase ◽  
Host Cell ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Substrate Recognition ◽  
Fever Virus ◽  
Valley Fever ◽  
Content Type ◽  
F Box Protein

ABSTRACTRift Valley fever virus (RVFV, familyBunyaviridae, genusPhlebovirus) is a relevant pathogen of both humans and livestock in Africa. The nonstructural protein NSs is a major virulence factor known to suppress the type I interferon (IFN) response by inhibiting host cell transcription and by proteasomal degradation of a major antiviral IFN effector, the translation-inhibiting protein kinase PKR. Here, we identified components of the modular SCF (Skp1, Cul1, F-box protein)-type E3 ubiquitin ligases as mediators of PKR destruction by NSs. Small interfering RNAs (siRNAs) against the conserved SCF subunit Skp1 protected PKR from NSs-mediated degradation. Consequently, RVFV replication was severely reduced in Skp1-depleted cells when PKR was present. SCF complexes have a variable F-box protein subunit that determines substrate specificity for ubiquitination. We performed an siRNA screen for all (about 70) human F-box proteins and found FBXW11 to be involved in PKR degradation. The partial stabilization of PKR by FBXW11 depletion upregulated PKR autophosphorylation and phosphorylation of the PKR substrate eIF2α and caused a shutoff of host cell protein synthesis in RVFV-infected cells. To maximally protect PKR from the action of NSs, knockdown of structurally and functionally related FBXW1 (also known as β-TRCP1), in addition to FBXW11 deletion, was necessary. Consequently, NSs was found to interact with both FBXW11 and β-TRCP1. Thus, NSs eliminates the antiviral kinase PKR by recruitment of SCF-type E3 ubiquitin ligases containing FBXW11 and β-TRCP1 as substrate recognition subunits. This antagonism of PKR by NSs is essential for efficient RVFV replication in mammalian cells.IMPORTANCERift Valley fever virus is a pathogen of humans and animals that has the potential to spread from Africa and the Arabian Peninsula to other regions. A major virulence mechanism is the proteasomal degradation of the antiviral kinase PKR by the viral protein NSs. Here, we demonstrate that NSs requires E3 ubiquitin ligase complexes of the SCF (Skp1, Cul1, F-box protein) type to destroy PKR. SCF-type complexes can engage variant ubiquitination substrate recognition subunits, and we found the F-box proteins FBXW11 and β-TRCP1 to be relevant for the action of NSs against PKR. Thus, we identified the host cell factors that are critically needed by Rift Valley fever virus to uphold its replication against the potent antiviral kinase PKR.

scholarly journals Chemotactic and Inflammatory Responses in the Liver and Brain Are Associated with Pathogenesis of Rift Valley Fever Virus Infection in the Mouse

2012 ◽  
Vol 6 (2) ◽  
pp. e1529 ◽  
Author(s):  
Kimberly K. Gray ◽  
Melissa N. Worthy ◽  
Terry L. Juelich ◽  
Stacy L. Agar ◽  
Allison Poussard ◽  
...  
Keyword(s):  
Virus Infection ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Inflammatory Responses ◽  
Rift Valley Fever Virus ◽  
Fever Virus ◽  
Valley Fever

scholarly journals Rift Valley Fever Virus Infection of Human Cells and Insect Hosts Is Promoted by Protein Kinase C Epsilon

PLoS ONE ◽  
2010 ◽  
Vol 5 (11) ◽  
pp. e15483 ◽  
Author(s):  
Claire Marie Filone ◽  
Sheri L. Hanna ◽  
M. Cecilia Caino ◽  
Shelly Bambina ◽  
Robert W. Doms ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Virus Infection ◽  
Rift Valley ◽  
Rift Valley Fever ◽  
Rift Valley Fever Virus ◽  
Human Cells ◽  
Valley Fever ◽  
Kinase C ◽  
Protein Kinase C Epsilon
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