scholarly journals Persistent Growth of a Human Plasma-Derived Hepatitis C Virus Genotype 1b Isolate in Cell Culture

2010 ◽  
Vol 6 (5) ◽  
pp. e1000910 ◽  
Author(s):  
Erica Silberstein ◽  
Kathleen Mihalik ◽  
Laura Ulitzky ◽  
Ewan P. Plant ◽  
Montserrat Puig ◽  
...  
Keyword(s):  
Cell Culture ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Human Plasma ◽  
Virus Genotype ◽  
Genotype 1B

scholarly journals Efficient Hepatitis C Virus Genotype 1b Core-NS5A Recombinants Permit Efficacy Testing of Protease and NS5A Inhibitors

2017 ◽  
Vol 61 (6) ◽  
Author(s):  
Long V. Pham ◽  
Santseharay Ramirez ◽  
Thomas H. R. Carlsen ◽  
Yi-Ping Li ◽  
Judith M. Gottwein ◽  
...  
Keyword(s):  
Cell Culture ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Resistance Testing ◽  
Replication Capacity ◽  
Genotype 1 ◽  
Virus Genotype ◽  
Culture Systems ◽  
Genotype 1B ◽  
Subtype 1B

ABSTRACT Hepatitis C virus (HCV) strains belong to seven genotypes with numerous subtypes that respond differently to antiviral therapies. Genotype 1, and primarily subtype 1b, is the most prevalent genotype worldwide. The development of recombinant HCV infectious cell culture systems for different variants, permitted by the high replication capacity of strain JFH1 (genotype 2a), has advanced efficacy and resistance testing of antivirals. However, efficient infectious JFH1-based cell cultures of subtype 1b are limited and comprise only the 5′ untranslated region (5′UTR)-NS2, NS4A, or NS5A regions. Importantly, it has not been possible to develop efficient 1b infectious systems expressing the NS3/4A protease, an important target of direct-acting antivirals. We developed efficient infectious JFH1-based cultures with genotype 1b core-NS5A sequences of strains DH1, Con1, and J4 by using previously identified HCV cell culture adaptive substitutions A1226G, R1496L, and Q1773H. These viruses spread efficiently in Huh7.5 cells by acquiring additional adaptive substitutions, and final recombinants yielded peak supernatant infectivity titers of 4 to 5 log10 focus-forming units (FFU)/ml. We subsequently succeeded in adapting a JFH1-based 5′UTR-NS5A DH1 recombinant to efficient growth in cell culture. We evaluated the efficacy of clinically relevant NS3/4A protease and NS5A inhibitors against the novel genotype 1b viruses, as well as against previously developed 1a viruses. The inhibitors were efficient against all tested genotype 1 viruses, with NS5A inhibitors showing half-maximal effective concentrations several orders of magnitude lower than NS3/4A protease inhibitors. In summary, the developed HCV genotype 1b culture systems represent valuable tools for assessing the efficacy of various classes of antivirals and for other virological studies requiring genotype 1b infectious viruses.

Efficacy and safety of elbasvir plus grazoprevir combination therapy in Japanese patients infected with hepatitis C virus genotype 1b

2018 ◽  
Vol 49 (3) ◽  
pp. 256-263 ◽  
Author(s):  
Yasue Takeuchi ◽  
Norio Akuta ◽  
Hitomi Sezaki ◽  
Fumitaka Suzuki ◽  
Shunichiro Fujiyama ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Combination Therapy ◽  
Japanese Patients ◽  
Efficacy And Safety ◽  
Virus Genotype ◽  
Genotype 1B

Serum-free cell culture system supplemented with lipid-rich albumin for hepatitis C virus (strain O of genotype 1b) replication

2007 ◽  
Vol 125 (2) ◽  
pp. 162-168 ◽  
Author(s):  
Ken-ichi Abe ◽  
Masanori Ikeda ◽  
Yasuo Ariumi ◽  
Hiromichi Dansako ◽  
Nobuyuki Kato
Keyword(s):  
Cell Culture ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Virus Strain ◽  
Culture System ◽  
Free Cell ◽  
Cell Culture System ◽  
Serum Free ◽  
Genotype 1B
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