scholarly journals Study of chromium, selenium and bromine concentrations in blood serum of patients with parenteral nutrition treatment using total reflection X-ray fluorescence analysis

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243492
Author(s):  
Monika Pierzak ◽  
Aldona Kubala-Kukuś ◽  
Dariusz Banaś ◽  
Ilona Stabrawa ◽  
Jolanta Wudarczyk-Moćko ◽  
...  
Keyword(s):  
Parenteral Nutrition ◽  
Blood Serum ◽  
Standard Procedure ◽  
Fluorescence Analysis ◽  
Selenium Supplementation ◽  
Total Reflection ◽  
Control Group ◽  
Serum Samples ◽  
X Ray ◽  
And Control

Total reflection X-ray fluorescence analysis (TXRF) was used to determine chromium, selenium and bromine concentrations in blood serum samples of 50 patients with parenteral nutrition treatment. The concentrations were measured two times, namely in the first day (I measurement) of the treatment and the seventh day (II measurement) after the chromium and selenium supplementation. For comparison purposes also serum samples of 50 patients without nutritional disorders, admitted to a planned surgical procedure to remove the gall bladder (cholecystectomy), were analyzed and treated as the control group. Descriptive statistics of measured concentrations of Cr, Se and Br both for the studied and control groups was determined. In order to check the effectiveness of Cr and Se supplementation, the results of the first and seventh day measurements for studied group were statistically compared with each other, with literature reference values and with the results of the control group (two-group comparison). These comparisons indicate the effectiveness of selenium supplementation in the applied treatment procedure. In the case of Cr and Br concentrations no statistically significant differences were observed. We conclude that monitoring of the concentration of the important trace elements in human serum should be standard procedure in parenteral nutrition treatment. In this monitoring the TXRF technique can be successfully used.

scholarly journals Analysis of Bowel Diseases from Blood Serum by Autofluorescence and Atomic Force Microscopy Techniques

2018 ◽  
Vol 16 (1) ◽  
pp. 238-245 ◽  
Author(s):  
Vladimíra Tomečková ◽  
Štefan Tóth ◽  
Tímea Tóth ◽  
Vladimír Komanický ◽  
Kristína Krajčíková ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Blood Serum ◽  
Fluorescence Analysis ◽  
Colon Resection ◽  
Diagnostic Methods ◽  
Control Group ◽  
Serum Samples ◽  
Force Microscopy ◽  
Atomic Force ◽  
Bowel Diseases

AbstractDiagnosis of bowel diseases is often difficult and time consuming since it is not always possible to obtain adequate information by the conventional diagnostic methods to set up a diagnosis and exclude nongastrointestinal causes of symptoms. The aim of this study was to investigate the structure of blood serum samples of patients with selected intestinal diseases. The blood serum samples of patients (N=35) with selected diagnoses (mesenteric thrombosis, inflammatory bowel disease, duodenal ulcers, sepsis, enterorrhagia, sigmoid colon resection, small intestine cancer) and of healthy subjects were evaluated by synchronous fluorescence fingerprint and atomic force microscopy. Autofluorescence of blood serum studied at λex = 280 nm showed significant decrease of fluorescence intensity in patients with all types of diseases affecting bowels in comparison with the healthy control patients. The blood serum surface of ill patients showed significant differences in comparison with control group samples after atomic force microscopy evaluation as well. Irregularly placed small globular units of irregular shape in small amounts are possible to observe in patients with intestine ischemia. Fluorescence analysis and atomic force microscopy showed the ability to rapidly reflect qualitative and quantitative changes of proteins in blood serum samples of patients. These sensitive methods could be beneficial for monitoring the progression of both acute or chronic bowel diseases.

scholarly journals The Relationship between Selenium and T3 in Selenium Supplemented and Nonsupplemented Ewes and Their Lambs

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Abd Elghany Hefnawy ◽  
Seham Youssef ◽  
P. Villalobos Aguilera ◽  
C. Valverde Rodríguez ◽  
J. L. Tórtora Pérez
Keyword(s):  
Positive Relationship ◽  
Selenium Concentration ◽  
Maternal Plasma ◽  
Selenium Supplementation ◽  
Control Group ◽  
Serum Samples ◽  
Plasma Selenium ◽  
Serum T3 ◽  
And Control ◽  
The Relationship

Twenty pregnant ewes were selected and classified into two groups. The first group received subcutaneous selenium supplementation (0.1 mg of sodium selenite/kg BW) at the 8th and 5th weeks before birth and 1st week after birth while the other was control group without selenium injection. Maternal plasma and serum samples were collected weekly from the 8th week before birth until the 8th week after birth and milk samples were taken from ewes weekly, while plasma and serum samples were collected at 48 hours, 1st, 2nd, 3rd, 5th, and 8th weeks after birth from the newborn lambs. Results demonstrated significant positive relationship between maternal plasma selenium and serum T3 in supplemented and control ewes (r=0.69to 0.72,P<0.05). There was significant (P<0.001) increase in T3 in supplemented ewes and their lambs until the 8th week after birth. There was positive relationship between milk, selenium concentration, and serum T3 in the newborn lambs of the supplemented group (r=0.84,P<0.01), while the relationship was negative in the control one (r=-0.89,P<0.01). Muscular and thyroid pathological changes were independent of selenium supplementation. Selenium supplementation was important for maintaining T3 in ewes and newborn lambs until the 8th week after birth.

How to Use the Features of Total Reflection of X-Rays for Energy Dispersive XRF

1988 ◽  
Vol 32 ◽  
pp. 105-114 ◽  
Author(s):  
H. Schwenke ◽  
W. Berneike ◽  
J. Knoth ◽  
U. Weisbrod
Keyword(s):  
Thin Films ◽  
Penetration Depth ◽  
Fluorescence Analysis ◽  
Total Reflection ◽  
X Rays ◽  
X Ray ◽  
Characteristic Features ◽  
Respective Characteristic ◽  
Nanometer Regime ◽  
Sensitive Method

AbstractThe total reflection of X-rays is mainly determined by three parameters , that is the orltical angle, the reflectivity and the penetration depth. For X-ray fluorescence analysis the respective characteristic features can be exploited in two rather different fields of application. In the analysis of trace elements in samples placed as thin films on optical flats, detection limits as low as 2 pg or 0.05 ppb, respectively, have been obtained. In addition, a penetration depth in the nanometer regime renders Total Reflection XRF an inherently sensitive method for the elemental analysis of surfaces. This paper outlines the main physical and constructional parameters for instrumental design and quantitation in both branches of TXRF.

Selective Mercury Determination after Membrane Complexation and Total Reflection X-ray Fluorescence Analysis

2004 ◽  
Vol 76 (15) ◽  
pp. 4315-4319 ◽  
Author(s):  
Pavlos E. Koulouridakis ◽  
Nikolaos G. Kallithrakas-Kontos
Keyword(s):  
Fluorescence Analysis ◽  
Total Reflection ◽  
Mercury Determination ◽  
X Ray

Trace Element Determination in Diesel Particulates by Total-Reflection X-Ray Fluorescence Analysis

1999 ◽  
Vol 131 (3-4) ◽  
pp. 219-223 ◽  
Author(s):  
Juha K. Vilhunen ◽  
Alex von Bohlen ◽  
Martina Schmeling ◽  
Leena Rantanen ◽  
Seppo Mikkonen ◽  
...  
Keyword(s):  
Trace Element ◽  
Fluorescence Analysis ◽  
Total Reflection ◽  
Trace Element Determination ◽  
Element Determination ◽  
X Ray

Main Peak Profiles of Total Reflection X-Ray Fluorescence Analysis of Si(001) Wafers Excited by Monochromatic X-Ray Beam W-Lβ (II)

1993 ◽  
Vol 32 (Part 1, No. 3A) ◽  
pp. 1191-1196 ◽  
Author(s):  
Kenji Yakushiji ◽  
Shinji Ohkawa ◽  
Atsushi Yoshinaga ◽  
Jimpei Harada
Keyword(s):  
Fluorescence Analysis ◽  
Total Reflection ◽  
Main Peak ◽  
X Ray

scholarly journals Evaluation of aqueous humor and serum clusterin levels in patients with glaucoma

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Fuat Yavrum ◽  
Ufuk Elgin ◽  
Zeynep Adiyaman Kocer ◽  
Vildan Fidanci ◽  
Emine Sen
Keyword(s):  
Anterior Chamber ◽  
Aqueous Humor ◽  
Open Angle Glaucoma ◽  
Control Group ◽  
Posterior Chamber ◽  
Serum Samples ◽  
Chi Square ◽  
New Finding ◽  
High Level ◽  
And Control

Abstract Background To compare the aqueous humor (AH) and the serum clusterin levels of patients with pseudoexfoliation syndrome (PEX), pseudoexfoliation glaucoma (PEXG), and primary open-angle glaucoma (POAG) with each other and with an age- and sex-matched control group. Methods This prospective, cross-sectionalstudy evaluated 92 eyes from 92 adult cases of uncomplicated phacoemulsification and posterior chamber intraocular lens (IOL) implantation. The cases were divided into PEX, PEXG, POAG, and control groups. Serum samples were taken from the antecubital vein just before the surgery, and the AH samples were aspirated at the beginning of the surgery. Kruskal-Wallis H, One-way ANOVA, Mann-Whitney U with Bonferroni correction and Chi-Square tests were used for statistical analysis. Results The serum clusterin levels were the highest in the PEXG group, but no statistically significant differences were observed between the groups (p=0.633). The mean AH clusterin levels were 286.79±29.64 μg/mL in the PEXG group, 263.92±31.70 μg/mL in the PEX group, 272.59±49.71 μg/mL in the POAG group, and 193.50±62.38 μg/mL in the control group (p< 0.001). This came out to be 1.48 times increase for the PEXG group, 1.36 for the PEX group, and 1.41 for the POAG group when compared with the control subjects. Conclusions A higher level of clusterin in the anterior chamber was found to be associated with PEX and PEXG. In addition, a high level of anterior chamber clusterin in POAG, which is a new finding, showed that this molecule might be important not only in pseudoexfoliation, but also other types of glaucoma like POAG.

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