scholarly journals High nitrogen concentration alter microbial community in Allium fistulosum rhizosphere

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0241371
Author(s):  
Chen Zhao ◽  
Haifeng Ni ◽  
Lin Zhao ◽  
Lin Zhou ◽  
Orlando Borrás-Hidalgo ◽  
...  
Keyword(s):  
Microbial Community ◽  
Relative Abundance ◽  
Growth And Development ◽  
High Throughput Sequencing ◽  
Nitrogen Concentration ◽  
Soil Microbiome ◽  
Allium Fistulosum ◽  
Sequencing Analysis ◽  
Nitrogen Application ◽  
Welsh Onion

Welsh onion (Allium fistulosum L.) constitutes an important plant species cultivated in China due the benefits and applications in different areas. Moreover, nitrogen is an essential nutrient during the growth and development of plant. Here, we present the effects of nitrogen on soil microbiome in welsh onion plants. We used High-throughput sequencing analysis to determine the diversity and abundances of microbes associated to soil rhizosphere in welsh onion under the influence of nitrogen application. Nitrogen application significantly influenced in the diversity of fungal community. The relative abundance of Orbiliomycetes increased with the nitrogen concentration. Nitrogen application did not affect the diversity of bacterial community, whereas the relative abundance of Acidobacteria_Gp2, Verrucomicrobiae and Sphingobacteriia decreased with the nitrogen condition. In this work, we introduced evidences of the effect of nitrogen fertilization on microbial community in welsh onion rhizosphere, and the change of microbial community may interfere the growth and development of welsh onion.

scholarly journals Microbial community with the ability to biodegradation perchlorate in a bio-electrochemical reactor

2019 ◽  
Vol 118 ◽  
pp. 01021
Author(s):  
Yu Zhong ◽  
Li He ◽  
Guangyi Fu ◽  
Youze Xu ◽  
Lixia Xie ◽  
...  
Keyword(s):  
Microbial Community ◽  
Relative Abundance ◽  
High Throughput Sequencing ◽  
Removal Rate ◽  
Electrochemical Reactor ◽  
Hunan Province ◽  
Sequencing Analysis ◽  
Initial Concentration ◽  
Perchlorate Reduction ◽  
Class Level

In this study, the sediments from the Liuyang River (Hunan Province, China) were used as an inoculum to bio-reduction perchlorate in a bio-electrochemical reactor (BER). The efficient degradation of perchlorate was found in the BER by utilizing the hydrogen as electron donor. When the current intensity was 10 mA and HRT was 72 h, the removal rate of perchlorate (initial concentration was 5 mg/L) reached 84.13% and a removal flux of 178.68 mg/m2·d was achieved. High-throughput sequencing analysis confirmed that the biofilm in the reactor had been successfully acclimated, and the system could achieve perchlorate reduction effectively. Firmicutes and Bacteroidetes were the dominant phyla during inoculation phase, and Actinobacteriria, Proteobacteria, and Tenericutes also constituted a low proportion in the biofilm. Bacilli and Clostridia were dominant at class-level both in inoculum and biofilm, with the relative abundance about 56%-72% and 17%-23%, respectively. These results confirmed that the biofilm in the BER system had been successfully formed, and the BER system could biodegradation perchlorate effectively.

scholarly journals Analysis of Microbial Diversity and Variation Law During Xiaoqu Baijiu Fermentation using High-throughput Sequencing Technology

2021 ◽  
Author(s):  
Qing Wang ◽  
Xiaoqing Xiang ◽  
PengFei Wu ◽  
Guoqiang Han
Keyword(s):  
Microbial Community ◽  
Microbial Diversity ◽  
High Throughput ◽  
Relative Abundance ◽  
High Throughput Sequencing ◽  
Sequencing Technology ◽  
Theoretical Support ◽  
Brewing Process ◽  
Dominant Bacteria ◽  
Brewing Time

Abstract In this study, high-throughput sequencing (HTS) was used to compare and analyze the microbial diversity and variation law during the brewing process of xiaoqu Baijiu. The results showed that 34 phyla, 378 genera of bacteria and 4 phyla, 32 genera of fungi were detected. At the phylum level, Firmicutes, Proteobacteria, Bacteroidetes, Ascomycota and Bacteroidetes were the dominant groups. During the brewing process of xiaoqu Baijiu, the dominant bacteria were Weissella and unidentified Rickettsiales 2 days before brewing and Lactobacillus 3 days after brewing until the end of brewing. The dominant fungi were Rhizopus, Saccharomyces and Issatchenkia. The relative abundance of Rhizopus decreased with the extension of brewing time, while the relative abundance of Saccharomyces increased and became the dominant bacteria after the second day of brewing. This study revealed the diversity and variation of microbial community in the brewing process of xiaoqu Baijiu, and provide theoretical support and lay the foundation for future study on the contribution of microbial metabolism during brewing of xiaoqu Baijiu, thereby promote the development of xiaoqu baijiu industry.

scholarly journals Sugarcane-Legume Intercropping Can Enrich the Soil Microbiome and Plant Growth

2021 ◽  
Vol 5 ◽  
Author(s):  
Mukesh Kumar Malviya ◽  
Manoj Kumar Solanki ◽  
Chang-Ning Li ◽  
Zhen Wang ◽  
Yuan Zeng ◽  
...  
Keyword(s):  
Microbial Community ◽  
Plant Growth ◽  
Microbial Diversity ◽  
High Throughput Sequencing ◽  
Soil Microbes ◽  
Negative Impact ◽  
Soil Microbiome ◽  
Rrna Gene ◽  
Internal Transcribed Spacer Region ◽  
Sugarcane Plant

Soil microbes have a direct impact on plant metabolism and health. The current study investigates the comparative rhizobiome between sugarcane monoculture and sugarcane–soybean intercropping. A greenhouse experiment was performed with two treatments: (1) sugarcane monoculture and (2) sugarcane–soybean intercropped. We used a high-throughput sequencing (HTS) platform to analyze the microbial community. We used the 16S rRNA gene and internal transcribed spacer region primers to identify the microbial diversity. HTS results revealed that a total of 2,979 and 124 bacterial and fungal operational taxonomic units (OTUs) were observed, respectively. Microbial diversity results concluded that the intercropping system has a beneficial impact on soil microbes. The highest numbers of bacterial and fungal OTUs were found in the intercropping system, and these results also collaborated with quantitative PCR results. Additionally, intercropped sugarcane plants showed a higher weight of above- and below-ground parts than the monoculture. Soil chemical analysis results also complemented that the intercropping system nourished organic carbon, total nitrogen, and soil enzyme activities. Correlation analysis of the diversity index and abundance concluded that soil nutrient content positively influenced the microbial abundance that improves plant growth. The present study frames out the profound insights of microbial community interaction under the sugarcane–soybean intercropping system. This information could help improve or increase the sugarcane crop production without causing any negative impact on sugarcane plant growth and development.

Analysis of microbial changes in the rhizosphere of unplanted and planted Atractylodes lancea via high-throughput sequencing

2020 ◽  
Author(s):  
Yan Xu ◽  
Junfeng Niu ◽  
Lijun Chen ◽  
Xiaoqiang Wu ◽  
Zhongmin Dong ◽  
...  
Keyword(s):  
Microbial Community ◽  
High Throughput ◽  
Relative Abundance ◽  
High Throughput Sequencing ◽  
Community Diversity ◽  
Continuous Cropping ◽  
Pests And Diseases ◽  
Atractylodes Lancea ◽  
Bacteria And Fungi ◽  
The Relationship

Abstract Background Atractylodes lancea is a traditional Chinese medicine, which typically requires more than 3–4 years of continuous cropping to obtain the underground medicinal components. With continuous cropping years, the quality and yields of A. lancea medicinal materials decrease, while pests and diseases increase. These aspects are intimately correlated with rhizospheric microorganisms. Methods This research paper employed high-throughput sequencing for its detection in soil that was cultivated for three years and never cultivated to clarify the relationship between the microbial diversity of the rhizosphere and continuous A. lancea cropping. Results The rhizosphere microbial community was altered following the continuous cropping of A. lancea. The bacterial diversity and richness were observed to decrease, while the fungal community diversity increased, and richness decreased. The total OUTs of the soil bacteria and fungi of unplanted and planted A. lancea were 59.58% and 37.65%, respectively. At the phylum level, the relative abundance of Proteobacteria, Gemmatimonadetes, Acidobacteria and Chloroflexi decreased, whereas the relative abundance of Mortierellomycota increased. At the genus level, Bradyrhizobium, Striaticonidium, Dactylonectria, Sphingomonas, Burkholderiaceae, Rhodanobacter, Arthrobacter, Scleroderma, Mortierella and Penicillium were significantly different between the two sample groups. Conclusions Our results revealed that following the cultivation of A. lancea, the rhizospheric microbial community was altered. This study preliminarily determined the

scholarly journals High-throughput sequencing analysis of the microbial community in coastal intensive mariculture systems

2018 ◽  
Vol 83 ◽  
pp. 93-102 ◽  
Author(s):  
Jian-Hua Wang ◽  
Jian Lu ◽  
Yu-Xuan Zhang ◽  
Jun Wu ◽  
Cui Zhang ◽  
...  
Keyword(s):  
Microbial Community ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Sequencing Analysis

scholarly journals Analysis of microbial community and pollutants removal performance in the simulated reaction pool by immobilized microbe technology

2019 ◽  
Vol 79 ◽  
pp. 03018 ◽  
Author(s):  
Qing Fang ◽  
Hai-fang Tang ◽  
Ping Xian ◽  
Jun-cen Bai
Keyword(s):  
Microbial Community ◽  
High Throughput Sequencing ◽  
Water Environment ◽  
Quality Standard ◽  
Control Group ◽  
Sequencing Analysis ◽  
Class Iii ◽  
Overlying Water ◽  
Pollutants Removal ◽  
Dominant Bacteria

In this lab-scale simulation, the performance of aerated immobilized microbe (IM) in in-situ pond sedimentation remediation, and the microbial community was studied. It is known for the results that total organic matter (TOM), total nitrogen (TN) and total phosphorus (TP) in the sediment can be effectively reduced by aerated immobilized microbe technology. The polluted raw water, classified as inferior Class-Ⅴ according to the surface water environment quality standard (GB3838-2002), upgraded to Class-III in 30-days remediation with TOM, TN and TP removal rates of 33.35%, 43.35% and 23.20%, respectively. In contract, the control group without the assistant of aerated immobilized microbe still remains at Class-IV. High-throughput sequencing analysis reveals that immobilized microbe technology could improve the species diversity and abundance of the flora, and enhance the similarity between the sediment and the overlying water in the system. The dominant bacteria of the overlying water were Perlucidibaca, Limnohabitans, Bacillus, Hydrogenophaga, Mycobacterium etc. And the dominant bacteria of the sediment were Methanolinea, Methanoregula, Candidatus Competibacter, Methanosaeta etc. Total phosphorous, certified by the redundancy analysis, is the key environmental factor of microbial community structure in both overlying water and sediment.

scholarly journals Correction: High nitrogen concentration alter microbial community in Allium fistulosum rhizosphere

PLoS ONE ◽  
2021 ◽  
Vol 16 (1) ◽  
pp. e0246163
Author(s):  
Chen Zhao ◽  
Haifeng Ni ◽  
Lin Zhao ◽  
Lin Zhou ◽  
Orlando Borrás-Hidalgo ◽  
...  
Keyword(s):  
Microbial Community ◽  
Nitrogen Concentration ◽  
Allium Fistulosum ◽  
High Nitrogen ◽  
High Nitrogen Concentration

scholarly journals Nitrogen Removal Efficiency and Microbial Community Analysis of a High-Efficiency Honeycomb Fixed-Bed Bioreactor

Water ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 1832
Author(s):  
Jie Xu ◽  
Chao Zhu ◽  
Yi Liu ◽  
Guanghui Lv ◽  
Changyan Tian ◽  
...  
Keyword(s):  
Microbial Community ◽  
Removal Efficiency ◽  
Nitrogen Removal ◽  
High Throughput Sequencing ◽  
High Efficiency ◽  
Removal Rate ◽  
Fixed Bed ◽  
Microbial Community Analysis ◽  
Sequencing Analysis ◽  
Fixed Bed Bioreactor

Based on the concept of microbial community multi-processing in integrated spatial bacterial succession (ISBS), this study constructs a highly efficient cellular fixed-bed bioreactor that follows the growth of biological flora in the wastewater treatment process. The reactor is organically partitioned based on synergistic laws and in accordance with environmental and microbial metabolic changes, and sewage is subjected to unitized and specialized biological treatment under direct current conditions. The results show that the ISBS reactor exhibits stable nitrogen removal performance under a low-carbon source. Compared with traditional sewage biochemical treatment technology, the microbial concentration is increased by 2–3 times and even up to 12 times, and the ammonia nitrogen removal rate is maintained at 99%. The removal rate reaches 90% (hydraulic retention time of 14 h). High-throughput sequencing analysis based on 16S rDNA reveals the microbial community structure succession at different depths of the same section of the reactor. The microbial community is rich under the influence of environmental factors and exhibits different responses. The intervals vary. An analysis of the microbial community function explains why the ISBS reactor has high nitrogen removal efficiency.

scholarly journals Effects of Andrographolide on Mouse Intestinal Microflora Based on High-Throughput Sequence Analysis

2021 ◽  
Vol 8 ◽  
Author(s):  
Haigang Wu ◽  
Xian Wu ◽  
Li Huang ◽  
Chongmei Ruan ◽  
Jinni Liu ◽  
...  
Keyword(s):  
Microbial Community ◽  
Gut Microbiota ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Bacterial Resistance ◽  
Microbial Community Composition ◽  
Intestinal Flora ◽  
Sequencing Analysis ◽  
Beneficial Bacteria ◽  
Intestinal Microbes

The intestinal flora is a micro-ecosystem that is closely linked to the overall health of the host. We examined the diversity and abundance of intestinal microorganisms in mice following the administration of andrographolide, a component of the Chinese medical herb Andrographis paniculata. Administration of andrographolide produces multiple beneficial effects including anti-inflammatory, antiviral and antibacterial effects but whether it directly influences the gut microbiota is not known. This study investigated whether the oral administration of andrographolide influences the intestinal microbiota and was compared with amoxicillin treatment as a positive control and water only as a negative control. We examined 21 cecal samples and conducted a high-throughput sequencing analysis based on V3-V4 variable region of the 16S rDNA genes. We found that the diversity and abundance of mouse gut microbiota decreased in direct proportion with the amoxicillin dose whereas andrographolide administration did not affect intestinal microbial community structure. The composition of intestinal microbes following andrographolide treatment was dominated by the Firmicutes while Bacteroidetes dominated the amoxicillin treatment group compared with the negative controls. Specifically, the f__Lachnospiraceae_ Unclassified, Lachnospiraceae_ NK4A136_group and Ruminococcaceae_ UCG-014 were enriched with andrographolide administration while Bacteroides, Klebsiella and Escherichia-Shigella significantly increased in the amoxicillin test groups. Amoxicillin administration altered the microbial community composition and structure by increasing the proportion of pathogenic to beneficial bacteria whereas andrographolide administration led to increases in the proportions and abundance of beneficial bacteria. This study provides a theoretical basis for finding alternatives to antibiotics to decrease bacterial resistance and restore intestinal floral imbalances.

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