scholarly journals Thermal cycling protects SH-SY5Y cells against hydrogen peroxide and β-amyloid-induced cell injury through stress response mechanisms involving Akt pathway

PLoS ONE ◽  
2020 ◽  
Vol 15 (10) ◽  
pp. e0240022
Author(s):  
Wei-Ting Chen ◽  
Yu-Yi Kuo ◽  
Guan-Bo Lin ◽  
Chueh-Hsuan Lu ◽  
Hao-Ping Hsu ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Stress Response ◽  
Thermal Cycling ◽  
Cell Injury ◽  
Akt Pathway ◽  
Β Amyloid

scholarly journals Thermal cycling protects SH-SY5Y cells against hydrogen peroxide and β-amyloid-induced cell injury through stress response mechanisms involving Akt pathway

2019 ◽  
Author(s):  
Wei-Ting Chen ◽  
Yu-Yi Kuo ◽  
Guan-Bo Lin ◽  
Chueh-Hsuan Lu ◽  
Hao-Ping Hsu ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Injury ◽  
Neuroprotective Effect ◽  
World Health ◽  
Akt Pathway ◽  
Physical Stimulation ◽  
Temporary Relief ◽  
Β Amyloid ◽  
Underlying Mechanisms ◽  
Health Organization

AbstractNeurodegenerative diseases (NDDs) are becoming a major threat to public health, according to the World Health Organization (WHO). The most common form of NDDs is Alzheimer’s disease (AD), boasting 60-70% share. Although some debates still exist, excessive aggregation of β-amyloid protein (Aβ) and neurofibrillary tangles has been deemed one of the major causes for the pathogenesis of AD. A growing number of evidences from studies, however, have suggested that reactive oxygen species (ROS) also play a key role in the onset and progression of AD. Although scientists have had some understanding of the pathogenesis of AD, the disease still cannot be cured, with existing treatment only capable of providing a temporary relief at best, partly due to the obstacle of blood-brain barrier (BBB). The study was aimed to ascertain the neuroprotective effect of thermal cycle hyperthermia (TC-HT) against hydrogen peroxide (H2O2) and Aβ-induced cytotoxicity in SH-SY5Y cells. Treating cells with this physical stimulation beforehand significantly improved the cell viability and decreased the ROS content. The underlying mechanisms may be due to the activation of Akt pathway and the downstream antioxidant and prosurvival proteins. The findings manifest significant potential of TC-HT in neuroprotection, via inhibition of oxidative stress and cell apoptosis. It is believed that coupled with the use of drugs or natural compounds, this methodology can be even more effective in treating NDDs.

scholarly journals Effect of high-frequency low-intensity pulsed electric field on protecting SH-SY5Y cells against hydrogen peroxide and β-amyloid-induced cell injury via ERK pathway

PLoS ONE ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. e0250491
Author(s):  
Wei-Ting Chen ◽  
Guan-Bo Lin ◽  
Yu-Yi Kuo ◽  
Chih-Hsiung Hsieh ◽  
Chueh-Hsuan Lu ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Electric Field ◽  
High Frequency ◽  
Cell Injury ◽  
Neuroprotective Effect ◽  
Specific Area ◽  
Pulsed Electric Field ◽  
Erk Pathway ◽  
Low Intensity ◽  
Β Amyloid

As the most common type of neurodegenerative diseases (NDDs), Alzheimer’s disease (AD) is thought to be caused mainly by the excessive aggregation of β-amyloid protein (Aβ). However, a growing number of studies have found that reactive oxygen species (ROS) play a key role in the onset and progression of AD. The present study aimed to probe the neuroprotective effect of high-frequency low-intensity pulsed electric field (H-LIPEF) for SH-SY5Y cells against hydrogen peroxide (H2O2) and Aβ-induced cytotoxicity. By looking in a systematic way into the frequency- and amplitude-dependent neuroprotective effect of pulsed electric field (PEF), the study finds that H-LIPEF at 200 Hz produces the optimal protective effect for SH-SY5Y cells. The underlying mechanisms were confirmed to be due to the activation of extracellular signal-regulated kinase (ERK) pathway and the downstream prosurvival and antioxidant proteins. Because the electric field can be modified to focus on specific area in a non-contact manner, the study suggests that H-LIPEF holds great potential for treating NDDs, whose effect can be further augmented with the administering of drugs or natural compounds at the same time.

scholarly journals Effect of high-frequency low-intensity pulsed electric field on protecting SH-SY5Y cells against hydrogen peroxide and β-amyloid-induced cell injury via ERK pathway

2019 ◽  
Author(s):  
Wei-Ting Chen ◽  
Guan-Bo Lin ◽  
Yu-Yi Kuo ◽  
Chih-Hsiung Hsieh ◽  
Chueh-Hsuan Lu ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Electric Field ◽  
High Frequency ◽  
Cell Injury ◽  
Neuroprotective Effect ◽  
Specific Area ◽  
Pulsed Electric Field ◽  
Erk Pathway ◽  
Low Intensity ◽  
Β Amyloid

AbstractAs the most common type of neurodegenerative diseases (NDDs), Alzheimer’s disease (AD) is thought to be caused mainly by the excessive aggregation of β-amyloid protein (Aβ). However, a growing number of studies have found that reactive oxygen species (ROS) play a key role in the onset and progression of AD. The present study aimed to probe the neuroprotective effect of high-frequency low-intensity pulsed electric field (H-LIPEF) for SH-SY5Y cells against hydrogen peroxide (H2O2) and Aβ-induced cytotoxicity. By looking in a systematic way into the frequency- and amplitude-dependent neuroprotective effect of pulsed electric field (PEF), the study finds that H-LIPEF at 200 Hz produces the optimal protective effect for SH-SY5Y cells. The underlying mechanisms were confirmed to be due to the activation of extracellular signal-regulated kinase (ERK) pathway and the downstream prosurvival and antioxidant proteins. Because electric field can be modified to focus on specific area in a non-contact manner, the study proposes that H-LIPEF holds great potential for treating NDDs, whose effect can be further augmented with the administering of drugs or natural compounds at the same time.

scholarly journals RpoS-Dependent Stress Response and Exoenzyme Production in Vibrio vulnificus

2003 ◽  
Vol 69 (10) ◽  
pp. 6114-6120 ◽  
Author(s):  
A. Hülsmann ◽  
T. M. Rosche ◽  
I.-S. Kong ◽  
H. M. Hassan ◽  
D. M. Beam ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Stress Response ◽  
Mutant Strain ◽  
Environmental Changes ◽  
Vibrio Vulnificus ◽  
High Sensitivity ◽  
Striking Difference ◽  
Wild Type ◽  
Hydrolytic Activities ◽  
In The Wild

ABSTRACT Vibrio vulnificus is an estuarine bacterium capable of causing rapidly fatal infections through both ingestion and wound infection. Like other opportunistic pathogens, V. vulnificus must adapt to potentially stressful environmental changes while living freely in seawater, upon colonization of the oyster gut, and upon infection of such diverse hosts as humans and eels. In order to begin to understand the ability of V. vulnificus to respond to such stresses, we examined the role of the alternate sigma factor RpoS, which is important in stress response and virulence in many pathogens. An rpoS mutant of V. vulnificus strain C7184o was constructed by homologous recombination. The mutant strain exhibited a decreased ability to survive diverse environmental stresses, including exposure to hydrogen peroxide, hyperosmolarity, and acidic conditions. The most striking difference was a high sensitivity of the mutant to hydrogen peroxide. Albuminase, caseinase, and elastase activity were detected in the wild type but not in the mutant strain, and an additional two hydrolytic activities (collagenase and gelatinase) were reduced in the mutant strain compared to the wild type. Additionally, the motility of the rpoS mutant was severely diminished. Overall, these studies suggest that rpoS in V. vulnificus is important for adaptation to environmental changes and may have a role in virulence.

TheahpDgene ofCorynebacterium glutamicumplays an important role in hydrogen peroxide-induced oxidative stress response

2018 ◽  
Vol 165 (2) ◽  
pp. 197-204 ◽  
Author(s):  
Eun-Ji Hong ◽  
Haeri Jeong ◽  
Dong-Seok Lee ◽  
Younhee Kim ◽  
Heung-Shick Lee
Keyword(s):  
Oxidative Stress ◽  
Hydrogen Peroxide ◽  
Stress Response ◽  
Oxidative Stress Response

scholarly journals Identification of a Regulator That Controls Stationary-Phase Expression of Catalase-Peroxidase in Caulobacter crescentus

1999 ◽  
Vol 181 (19) ◽  
pp. 6152-6159 ◽  
Author(s):  
Paul S. Rava ◽  
Laura Somma ◽  
Howard M. Steinman
Keyword(s):  
Hydrogen Peroxide ◽  
Stress Response ◽  
Stationary Phase ◽  
Caulobacter Crescentus ◽  
Sequence Similarity ◽  
Enteric Bacteria ◽  
Cross Resistance ◽  
Peptide Antibiotic ◽  
Reading Frame ◽  
Catalase Peroxidase

ABSTRACT Expression of the catalase-peroxidase of Caulobacter crescentus, a gram-negative member of the α subdivision of theProteobacteria, is 50-fold higher in stationary-phase cultures than in exponential cultures. To identify regulators of the starvation response, Tn5 insertion mutants were isolated with reduced expression of a katG::lacZ fusion on glucose starvation. One insertion interrupted an open reading frame encoding a protein with significant amino acid sequence identity to TipA, a helix-turn-helix transcriptional activator in the response ofStreptomyces lividans to the peptide antibiotic thiostrepton, and lesser sequence similarity to other helix-turn-helix regulators in the MerR family. The C. crescentus orthologue of tipA was named skgA (stationary-phase regulation of katG). Stationary-phase expression ofkatG was reduced by 70% in theskgA::Tn5 mutant, and stationary-phase resistance to hydrogen peroxide decreased by a factor of 10. Like the wild type, the skgA mutant exhibited starvation-induced cross-resistance to heat and acid shock, entered into the helical morphology that occurs after 9 to 12 days in stationary phase, and during exponential growth inducedkatG in response to hydrogen peroxide challenge. Expression of skgA increased 5- to 10-fold in late exponential phase.skgA is the first regulator of a starvation-induced stress response identified in C. crescentus. SkgA is not a global regulator of the stationary-phase stress response; its action encompasses the oxidative stress-hydrogen peroxide response but not acid or heat responses. Moreover, SkgA is not an alternative ς factor, like RpoS, which controls multiple aspects of starvation-induced cross-resistance to stress in enteric bacteria. These observations raise the possibility that regulation of stationary-phase gene expression in this member of the α subdivision of the Proteobacteria is different from that inEscherichia coli and other members of the γ subdivision.

Hydrogen peroxide enhanced Ca2+-activated BK currents and promoted cell injury in human dermal fibroblasts

Life Sciences ◽  
2012 ◽  
Vol 90 (11-12) ◽  
pp. 424-431 ◽  
Author(s):  
Bing Feng ◽  
Wen-Lei Ye ◽  
Lai-ji Ma ◽  
Yun Fang ◽  
Yan-Ai Mei ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Cell Injury ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts

scholarly journals Neuroprotective Effects of Lindleyin on Hydrogen Peroxide-Induced Cell Injury and MPTP-Induced Parkinson’s Disease in C57BL/6 Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Jin-Jie Zhang ◽  
Xiao-Rong Shi ◽  
Wen-Wen Lv ◽  
Xiao-Long Zhou ◽  
Ying-Dong Sun ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Parkinson’S Disease ◽  
Hydrogen Peroxide ◽  
Parkinson's Disease ◽  
Cell Injury ◽  
In Vivo Studies ◽  
Antioxidant Enzyme Activities ◽  
Apoptotic Pathway ◽  
Neuroprotective Effects

Oxidative stress (OS) is a crucial factor influencing the development of Parkinson’s disease (PD). Here we first reported that Lindleyin (Lin), one of the major components of rhubarb, possessed neuroprotective effects against H2O2-induced SH-SY5Y cell injury and MPTP-induced PD of C57BL/6 mice. The results showed that Lin can decrease cell death and apoptotic rate induced by H2O2 through inhibiting mitochondrial apoptotic pathway and increasing the activities of SOD, GSH-Px, and CAT as well as decreasing the level of MDA. In addition, in vivo studies showed that oral administration of Lin (5 or 20 mg/kg) showed significant change in motor function deficits, antioxidant enzyme activities, apoptotic pathway, and tyrosine hydroxylase expression. Our results reveal that Lin might be a promising anti-PD agent by reducing OS and apoptosis.

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