scholarly journals Different murine-derived feeder cells alter the definitive endoderm differentiation of human induced pluripotent stem cells

PLoS ONE ◽  
2018 ◽  
Vol 13 (7) ◽  
pp. e0201239
Author(s):  
Masaki Shoji ◽  
Hiroki Minato ◽  
Soichiro Ogaki ◽  
Masahide Seki ◽  
Yutaka Suzuki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Definitive Endoderm ◽  
Feeder Cells ◽  
Endoderm Differentiation ◽  
Induced Pluripotent

scholarly journals Neural crest stem cells can be induced in vitro from human-induced pluripotent stem cells using a novel protocol free of feeder cells

2021 ◽  
Vol 16 (3) ◽  
pp. 143-147
Author(s):  
Rei Abe ◽  
Kazuyo Yamauchi ◽  
Kazuki Kuniyoshi ◽  
Takane Suzuki ◽  
Yusuke Matsuura ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Crest ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Feeder Cells ◽  
Neural Crest Stem Cells ◽  
Induced Pluripotent

Neural differentiation of mouse induced pluripotent stem cells using cadherin gene-engineered PA6 feeder cells

2019 ◽  
Vol 127 (5) ◽  
pp. 633-640 ◽  
Author(s):  
Paerwen Paerhati ◽  
Akira Ito ◽  
Kantaro Yoshioka ◽  
Kaori Iwamoto ◽  
Sho Fujiwara ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Neural Differentiation ◽  
Feeder Cells ◽  
Induced Pluripotent

scholarly journals Definitive Endoderm Formation from Plucked Human Hair-Derived Induced Pluripotent Stem Cells and SK Channel Regulation

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Anett Illing ◽  
Marianne Stockmann ◽  
Narasimha Swamy Telugu ◽  
Leonhard Linta ◽  
Ronan Russell ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Induced Pluripotent Stem Cells ◽  
Cell Fate ◽  
Pluripotent Stem Cells ◽  
Human Hair ◽  
Definitive Endoderm ◽  
Sk Channel ◽  
Induced Pluripotent

Pluripotent stem cells present an extraordinary powerful tool to investigate embryonic development in humans. Essentially, they provide a unique platform for dissecting the distinct mechanisms underlying pluripotency and subsequent lineage commitment. Modest information currently exists about the expression and the role of ion channels during human embryogenesis, organ development, and cell fate determination. Of note, small and intermediate conductance, calcium-activated potassium channels have been reported to modify stem cell behaviour and differentiation. These channels are broadly expressed throughout human tissues and are involved in various cellular processes, such as the after-hyperpolarization in excitable cells, and also in differentiation processes. To this end, human induced pluripotent stem cells (hiPSCs) generated from plucked human hair keratinocytes have been exploitedin vitroto recapitulate endoderm formation and, concomitantly, used to map the expression of the SK channel (SKCa) subtypes over time. Thus, we report the successful generation of definitive endoderm from hiPSCs of ectodermal origin using a highly reproducible and robust differentiation system. Furthermore, we provide the first evidence that SKCas subtypes are dynamically regulated in the transition from a pluripotent stem cell to a more lineage restricted, endodermal progeny.

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