scholarly journals Livestock-Associated, Antibiotic-Resistant Staphylococcus aureus Nasal Carriage and Recent Skin and Soft Tissue Infection among Industrial Hog Operation Workers

PLoS ONE ◽  
2016 ◽  
Vol 11 (11) ◽  
pp. e0165713 ◽  
Author(s):  
Maya Nadimpalli ◽  
Jill R. Stewart ◽  
Elizabeth Pierce ◽  
Nora Pisanic ◽  
David C. Love ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Soft Tissue Infection ◽  
Nasal Carriage ◽  
Tissue Infection ◽  
Antibiotic Resistant

scholarly journals CRISPR-Cas9 modified bacteriophage for treatment of Staphylococcus aureus induced osteomyelitis and soft tissue infection

2019 ◽  
Author(s):  
Leah K. Horstemeyer ◽  
JooYoun Park ◽  
Elizabeth A. Swanson ◽  
Mary Catherine Beard ◽  
Emily M. McCabe ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Soft Tissue Infection ◽  
Bone Infection ◽  
Tissue Infection ◽  
Fluorescent Imaging ◽  
Alginate Hydrogel ◽  
Antibiotic Resistant

AbstractOsteomyelitis, or bone infection, is often induced by antibiotic resistant Staphylococcus aureus strains of bacteria. Although debridement and long-term administration of antibiotics are the gold standard for osteomyelitis treatment, the increase in prevalence of antibiotic resistant bacterial strains limits the ability of clinicians to effectively treat infection. Bacteriophages (phages), viruses that effectively lyse bacteria, have gained recent attention for their high specificity, non-toxicity, and the low likelihood of resistance development by pathogens. Previously, we have shown that CRISPR-Cas9 genomic editing techniques could be utilized to expand bacteriophage host range and enhance bactericidal activity through modification of the tail fiber protein, as well as improve safety with removal of major virulence genes. In a dermal infection study, these CRISPR-Cas9 phages reduced bacterial load relative to unmodified phage. Thus, we hypothesized this bacteriophage would be effective to mitigate infection from a biofilm forming S. aureus strain in vitro and in vivo. In vitro, qualitative fluorescent imaging demonstrated superiority of phage to conventional vancomycin and fosfomycin antibiotics against S. aureus biofilm. Quantitative antibiofilm effects increased over time for fosfomycin, phage, and fosfomycin-phage (dual) therapeutics delivered via alginate hydrogel. We developed an in vivo rat model of osteomyelitis and soft tissue infection that was reproducible and challenging and enabled longitudinal monitoring of infection progression. Using this model, phage (with and without fosfomycin) delivered via alginate hydrogel were successful in reducing soft tissue infection but not bone infection, based on bacteriological, histological, and scanning electron microscopy analyses. Notably, the efficacy of phage at mitigating soft tissue infection was equal to that of high dose fosfomycin. Future research may utilize this model as a platform for evaluation of therapeutic type and dose, and alternate delivery vehicles for osteomyelitis mitigation.

Hospitalization of paediatric patients for methicillin-resistant Staphylococcus aureus skin and soft-tissue infection, 1998–2006

2009 ◽  
Vol 138 (5) ◽  
pp. 677-682 ◽  
Author(s):  
K. D. SIRCAR ◽  
E. BANCROFT ◽  
D. M. NGUYEN ◽  
L. MASCOLA
Keyword(s):  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Los Angeles ◽  
Soft Tissue Infection ◽  
International Classification Of Diseases ◽  
Incidence Rates ◽  
Tissue Infection ◽  
Predictive Values ◽  
Methicillin Resistant ◽  
Classification Of Diseases

SUMMARYHospital discharge reports have provided data for studies of methicillin-resistant Staphylococcus aureus (MRSA) skin and soft-tissue infection (SSTI) studies. This analysis determined the sensitivity and positive predictive value of International Classification of Diseases, Ninth Revision, Clinical Modification (ICD-9-CM) code combinations to calculate hospitalization incidence rates, representativeness of a set of three ICD-9-CM codes to define MRSA SSTI, and hospitalization incidence rate trends for paediatric MRSA SSTIs in Los Angeles County (LAC). Using 133 cases from 31 hospitals, we found that the set of three ICD-9-CM codes used to define laboratory-confirmed cases had one of the highest positive predictive values (49%). There was no difference in age and race between those categorized using three codes vs. other code combinations. A dramatic increase in paediatric MRSA SSTI cases occurred in LAC during 1998–2006. We conclude that this combination of codes may be used to determine the rise of MRSA SSTIs in paediatric populations.

scholarly journals Risk Factors Associated With Methicillin-Resistant Staphylococcus aureus (MRSA) Complicated Skin and Soft Tissue Infection in Colombia

2016 ◽  
Vol 3 (suppl_1) ◽  
Author(s):  
Sandra Liliana Valderrama Beltran ◽  
Sandra Gualtero ◽  
Jose Rodriguez ◽  
Johanna Osorio ◽  
Carlos Arturo Alvarez Moreno ◽  
...  
Keyword(s):  
Risk Factors ◽  
Staphylococcus Aureus ◽  
Soft Tissue ◽  
Soft Tissue Infection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Tissue Infection ◽  
Methicillin Resistant ◽  
Factors Associated ◽  
Complicated Skin
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