scholarly journals Effects of Granulocyte-Macrophage Colony-Stimulating (GM-CSF) Factor on Corneal Epithelial Cells in Corneal Wound Healing Model

PLoS ONE ◽  
2015 ◽  
Vol 10 (9) ◽  
pp. e0138020 ◽  
Author(s):  
Chang Rae Rho ◽  
Mi-young Park ◽  
Seungbum Kang
Keyword(s):  
Wound Healing ◽  
Epithelial Cells ◽  
Corneal Epithelial Cells ◽  
Corneal Wound Healing ◽  
Corneal Epithelial ◽  
Gm Csf ◽  
Corneal Wound ◽  
Wound Healing Model ◽  
Macrophage Colony ◽  
Healing Model

scholarly journals Caveolin-1 regulates corneal wound healing by modulating Kir4.1 activity

2016 ◽  
Vol 310 (11) ◽  
pp. C993-C1000 ◽  
Author(s):  
Chengbiao Zhang ◽  
Xiaotong Su ◽  
Lars Bellner ◽  
Dao-Hong Lin
Keyword(s):  
Wound Healing ◽  
Epithelial Cells ◽  
Western Blot ◽  
Negative Control ◽  
Promoting Effect ◽  
Corneal Epithelial Cells ◽  
Corneal Wound Healing ◽  
Corneal Epithelial ◽  
Caveolin 1 ◽  
Corneal Wound

The expression of caveolin-1 (Cav1) in corneal epithelium is associated with regeneration potency. We used Cav1−/− mice to study the role of Cav1 in modulating corneal wound healing. Western blot and whole cell patch clamp were employed to study the effect of Cav1 deletion on Kir4.1 current density in corneas. We found that Ba2+-sensitive K+ currents in primary cultured murine corneal epithelial cells (pMCE) from Cav1−/− were dramatically reduced (602 pA) compared with those from wild type (WT; 1,300 pA). As a consequence, membrane potential was elevated in pMCE from Cav1−/− compared with that from WT (−43 ± 7.5 vs. −58 ± 4.0 mV, respectively). Western blot showed that either inhibition of Cav1 expression or Ba2+ incubation stimulated phosphorylation of the EGFR. The transwell migration assay showed that Cav1 genetic inactivation accelerated cell migration. The regrowth efficiency of human corneal epithelial cells (HCE) transfected with siRNA-Cav1 or negative control was evaluated by scrape injury assay. With the presence of mitomycin C (10 μg/ml) to avoid the influence of cell proliferation, Cav1 inhibition with siRNA significantly increased migration compared with control siRNA in HCE. This promoting effect by siRNA-Cav1 could not be further enhanced by cotransfection with siRNA-Kcnj10. By using corneal debridement, we found that wound healing was significantly accelerated in Cav1−/− compared with WT mice (70 ± 10 vs. 36 ± 3%, P < 0.01). Our findings imply that the mechanism by which Cav-1 knockout promotes corneal regrowth is, at least partially, due to the inhibition of Kir4.1 which stimulates EGFR signaling.

scholarly journals Hyaluronate Acid-Dependent Protection and Enhanced Corneal Wound Healing against Oxidative Damage in Corneal Epithelial Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Jing Zhong ◽  
Yuqing Deng ◽  
Bishan Tian ◽  
Bowen Wang ◽  
Yifang Sun ◽  
...  
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Epithelial Cells ◽  
Cell Apoptosis ◽  
Corneal Epithelial Cell ◽  
Corneal Epithelial Cells ◽  
Corneal Wound Healing ◽  
Corneal Epithelial ◽  
Expression Levels ◽  
Corneal Wound

Purpose. To evaluate the effects and mechanism of exogenous hyaluronate (HA) in promoting corneal wound healing.Methods. Human corneal epithelial cells (HCECs) were incubated with different concentrations of HA to evaluate their efficiency in promoting cell migration and their modulation of repair factors. After inducing hyperosmolar conditions, the cell morphologies, cell apoptosis, and expression levels of TNF-αand MMP-9 were detected to assess the protective role of HA. Corneal epithelium-injured rat models were established to test the therapeutic effects of 0.3% HA. Then, the wound healing rates, the RNA expression levels of inflammatory cytokines, and repair factors were examined.Results. HCECs in the 0.03% and 0.3% HA groups showed fewer morphological alterations and lower rates of cell apoptosis following preincubation with HA under hyperosmolar conditions, as well as the expression levels of MMP-9 and TNF-α. In the rat model, the areas of fluorescein staining in the corneas of 0.3% HA group were significantly smaller than the control group. The expression levels of IL-1βand MMP-9 were decreased, while CD44 and FN were increased in the 0.3% HA group.Conclusion. HA enhanced corneal epithelial cell wound healing by promoting cell migration, upregulating repair responses, and suppressing inflammatory responses.

A Tissue-Engineered Corneal Wound Healing Model for the Characterization of Reepithelialization

2013 ◽  
pp. 59-78 ◽  
Author(s):  
Karine Zaniolo ◽  
Patrick Carrier ◽  
Sylvain L. Guérin ◽  
François A. Auger ◽  
Lucie Germain
Keyword(s):  
Wound Healing ◽  
Corneal Wound Healing ◽  
Corneal Wound ◽  
Wound Healing Model ◽  
Healing Model

Therapeutic effects of zerumbone in an alkali-burned corneal wound healing model

2017 ◽  
Vol 48 ◽  
pp. 126-134 ◽  
Author(s):  
Jong Won Kim ◽  
Hyuneui Jeong ◽  
Myeon-Sik Yang ◽  
Chae Woong Lim ◽  
Bumseok Kim
Keyword(s):  
Wound Healing ◽  
Therapeutic Effects ◽  
Corneal Wound Healing ◽  
Corneal Wound ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals Characterization of Wound Reepithelialization Using a New Human Tissue-Engineered Corneal Wound Healing Model

2008 ◽  
Vol 49 (4) ◽  
pp. 1376 ◽  
Author(s):  
Patrick Carrier ◽  
Alexandre Deschambeault ◽  
Marie`ve Talbot ◽  
Claude J. Giasson ◽  
Franc¸ois A. Auger ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Tissue ◽  
Corneal Wound Healing ◽  
Corneal Wound ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals NGF and TGF-β mRNA expression during pregnancy in a rat corneal wound healing model

2003 ◽  
Vol 86 (4) ◽  
pp. 239-243 ◽  
Author(s):  
A Nieto ◽  
J Casas ◽  
SM Rahhal ◽  
L Alonso ◽  
F Martinez-Soriano ◽  
...  
Keyword(s):  
Wound Healing ◽  
Mrna Expression ◽  
Corneal Wound Healing ◽  
Corneal Wound ◽  
Wound Healing Model ◽  
Healing Model

scholarly journals Matrix regeneration agents improve wound healing in non-stressed human corneal epithelial cells

Eye ◽  
2017 ◽  
Vol 32 (4) ◽  
pp. 813-819 ◽  
Author(s):  
A Robciuc ◽  
R P J Arvola ◽  
M Jauhiainen ◽  
J M Holopainen
Keyword(s):  
Wound Healing ◽  
Epithelial Cells ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Human Corneal Epithelial Cells ◽  
Improve Wound Healing

A nano-phytochemical ophthalmic solution for marked improvement of corneal wound healing in healthy or diabetic mice

Nanomedicine ◽  
2021 ◽  
Author(s):  
Qiqi Li ◽  
Meng Xin ◽  
Xianggen Wu ◽  
Bo Lei
Keyword(s):  
Wound Healing ◽  
Ophthalmic Solution ◽  
Diabetic Mice ◽  
Promoting Effect ◽  
Related Factors ◽  
Corneal Wound Healing ◽  
Corneal Epithelial ◽  
Trigeminal Neurons ◽  
Corneal Wound

Aim: To formulate a novel nano-phytochemical ophthalmic solution to promote corneal wound healing. Methods: Dipotassium glycyrrhizinate (DG) and palmatine (PAL) were used to formulate this formulation marked as DG-PAL, and its efficacy and mechanisms for promoting corneal wound healing were evaluated in mice. Results: DG-PAL was easily fabricated with excellent physical profiles. In in vivo efficiency evaluations, DG-PAL demonstrated an excellent promoting effect on corneal epithelial/nerve wound healing in both healthy and diabetic mice. These effects were involved in the DG-PAL-induced decreased expression levels of HMGB1 and its signaling-related factors in the corneas and trigeminal neurons of the healthy or diabetic mice. Conclusion: DG-PAL possibly represents a promising ophthalmic solution for promoting corneal wound healing.

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