scholarly journals Transcriptome Analysis of Indole-3-Butyric Acid-Induced Adventitious Root Formation in Nodal Cuttings of Camellia sinensis (L.)

PLoS ONE ◽  
2014 ◽  
Vol 9 (9) ◽  
pp. e107201 ◽  
Author(s):  
Kang Wei ◽  
Li-Yuan Wang ◽  
Li-Yun Wu ◽  
Cheng-Cai Zhang ◽  
Hai-Lin Li ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Camellia Sinensis ◽  
Transcriptome Analysis ◽  
Adventitious Root ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
Nodal Cuttings

scholarly journals Highly Successful Adventitious Root Formation of Zamia L. Stem Cuttings Exhibits Minimal Response to Indole-3-Butyric Acid

HortScience ◽  
2020 ◽  
Vol 55 (9) ◽  
pp. 1463-1467 ◽  
Author(s):  
Benjamin E. Deloso ◽  
Anders J. Lindström ◽  
Frank A. Camacho ◽  
Thomas E. Marler
Keyword(s):  
Butyric Acid ◽  
Root Development ◽  
Adventitious Root ◽  
Adventitious Roots ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
The Other ◽  
Control Group ◽  
Stem Cuttings ◽  
Adventitious Root Development

The influences of indole-3-butyric acid (IBA) concentrations of 0–30 mg·g−1 on the success and speed of adventitious root development of Zamia furfuracea L.f. and Zamia integrifolia L.f. stem cuttings were determined. Root formation success for both species was greater than 95%. The IBA concentrations did not influence the speed of root development for Z. furfuracea, but the Z. integrifolia cuttings that received IBA concentration of 3 mg·g−1 generated adventitious roots more slowly than the cuttings in the control group. The ending dry weights of the stems, leaves, and roots were not influenced by IBA concentration for either species. Our results indicated that adventitious root formation on stem cuttings of these two Zamia species is successful without horticultural application of IBA. Additional IBA studies are needed on the other 300+ cycad species, especially those that are in a threatened category.

scholarly journals Transcriptomic profiling and discovery of key genes involved in adventitious root formation from green cuttings of highbush blueberry (Vaccinium corymbosum L.)

2020 ◽  
Author(s):  
Haishan An ◽  
Jiaying Zhang ◽  
Fangjie Xu ◽  
Shuang Jiang ◽  
Xueying Zhang
Keyword(s):  
Candidate Genes ◽  
Transcriptome Analysis ◽  
Adventitious Root ◽  
Root Formation ◽  
Transcriptome Profiling ◽  
Vaccinium Corymbosum ◽  
Adventitious Root Formation ◽  
Root Characteristics ◽  
Indole Butyric Acid ◽  
Root Primordium

Abstract Background: Propagation of cuttings is frequently used in various plant species, including blueberry, which shows special root characteristics that may hinder adventitious root (AR) formation. AR formation is influenced by various factors, and auxin is considered to play a central role; however, little is known of the related regulatory mechanisms. In this study, a comparative transcriptome analysis of green cuttings treated with or without indole-butyric acid (IBA) was performed via RNA_seq to identify candidate genes associated with IBA-induced AR formation.Results: Rooting phenotypes, especially the rooting rate, were significantly promoted by exogenous auxin in the IBA application. Blueberry AR formation was an auxin-induced process, during which adventitious root primordium initiation (rpi) began at 14 days after cutting (DAC), root primordium (rp) was developed at 21 DAC, mature AR was observed at 28 DAC and finally outgrowth from the stem occurred at 35 DAC. Higher IAA levels and lower ABA and zeatin contents might facilitate AR formation and development. A time series transcriptome analysis identified 14970 differentially expressed genes (DEGs) during AR formation, of which there were 7467 upregulated and 7503 downregulated genes. Of these, approximately 35 candidate DEGs involved in the auxin-induced pathway and AR formation were further identified, including 10 auxin respective genes (ARFs and SAURs), 13 transcription factors (LOB domain-containing protein (LBDs)), 6 auxin transporters (AUX22, LAX3/5 and PIN-like 6 (PIL6s)) and 6 rooting-associated genes (root meristem growth factor 9 (RGF9), lateral root primordium 1 (LRP1s), and dormancy-associated protein homologue 3 (DRMH3)). All these identified DEGs were highly upregulated in certain stages during AR formation, indicating their potential roles in blueberry AR formation.Conclusions: The transcriptome profiling results indicated candidate genes or major regulatory factors that influence adventitious root formation in blueberry and provided a comprehensive understanding of the rooting mechanism underlying the auxin-induced AR formation from blueberry green cuttings.

scholarly journals Transcriptomic profiling and discovery of key genes involved in adventitious root formation from green cuttings of highbush blueberry (Vaccinium corymbosum L.)

2020 ◽  
Author(s):  
Haishan An ◽  
Jiaying Zhang ◽  
Fangjie Xu ◽  
Shuang Jiang ◽  
Xueying Zhang
Keyword(s):  
Candidate Genes ◽  
Transcriptome Analysis ◽  
Adventitious Root ◽  
Root Formation ◽  
Transcriptome Profiling ◽  
Vaccinium Corymbosum ◽  
Adventitious Root Formation ◽  
Root Characteristics ◽  
Indole Butyric Acid ◽  
Root Primordium

Abstract Background: Propagation of cuttings is frequently used in various plant species, including blueberry, which shows special root characteristics that may hinder adventitious root (AR) formation. AR formation is influenced by various factors, and auxin is considered to play a central role; however, little is known of the related regulatory mechanisms. In this study, a comparative transcriptome analysis of green cuttings treated with or without indole-butyric acid (IBA) was performed via RNA_seq to identify candidate genes associated with IBA-induced AR formation. Results: Rooting phenotypes, especially the rooting rate, were significantly promoted by exogenous auxin in the IBA application. Blueberry AR formation was an auxin-induced process, during which adventitious root primordium initiation (rpi) began at 14 days after cutting (DAC), root primordium (rp) was developed at 21 DAC, mature AR was observed at 28 DAC and finally outgrowth from the stem occurred at 35 DAC. Higher IAA levels and lower ABA and zeatin contents might facilitate AR formation and development. A time series transcriptome analysis identified 14970 differentially expressed genes (DEGs) during AR formation, of which there were 7467 upregulated and 7503 downregulated genes. Of these, approximately 35 candidate DEGs involved in the auxin-induced pathway and AR formation were further identified, including 10 auxin respective genes ( ARFs and SAURs ), 13 transcription factors ( LOB domain-containing protein ( LBD s)), 6 auxin transporters ( AUX22 , LAX3/5 and PIN-like 6 ( PIL6s )) and 6 rooting-associated genes ( root meristem growth factor 9 ( RGF9 ), lateral root primordium 1 ( LRP1s ), and dormancy-associated protein homologue 3 ( DRMH3 )). All these identified DEGs were highly upregulated in certain stages during AR formation, indicating their potential roles in blueberry AR formation. Conclusions: The transcriptome profiling results indicated candidate genes or major regulatory factors that influence adventitious root formation in blueberry and provided a comprehensive understanding of the rooting mechanism underlying the auxin-induced AR formation from blueberry green cuttings.

scholarly journals Transcriptomic profiling and discovery of key genes involved in adventitious root formation from green cuttings of highbush blueberry (Vaccinium corymbosum L.)

2019 ◽  
Author(s):  
Haishan An ◽  
Jiaying Zhang ◽  
Fangjie Xu ◽  
Shuang Jiang ◽  
Xueying Zhang
Keyword(s):  
Candidate Genes ◽  
Transcriptome Analysis ◽  
Adventitious Root ◽  
Root Formation ◽  
Transcriptome Profiling ◽  
Vaccinium Corymbosum ◽  
Adventitious Root Formation ◽  
Highbush Blueberry ◽  
Root Characteristics ◽  
Root Primordium

Abstract Background Propagation of cuttings was mostly used in various plant species including blueberry, the special root characteristics of blueberry usually resulted in a difficulty in adventitious root (AR) formation. The AR formation was influenced by various factors, of which auxin was considered to play a center role, however little is known of the related regulative mechanisms. In this study, transcriptome analysis using RNA_seq from the stem of green cuttings of southern highbush blueberry 'Biloxi' was performed to discover candidate genes associated with AR formation.Results Rooting phenotypes, especially rooting rate, was significantly promoted by exogenous auxin IBA application. The adventitious root primordium initiation (rpi) began to be formed at 14 day (d) after cutting, developed into root primordium (rp) at 21d, finally the rp further developed to mature AR at 28d. Higher IAA and lower ABA and zeatin might facilitate the AR formation and development. A time series transcriptome analysis indentified 14970 differentially expressed genes (DEGs) during AR formation, of which there were 7467 up-regulated and 7503 down-regulated genes, respectively. Of these, about 35 candidate DEGs involved in auxin-induced pathway and AR formation were further identified, including 10 auxin respective genes ARFs and SAURs , 13 transcription factors LOB domain-containing protein ( LBD s), 6 auxin transporter AUX22 , LAX3/5 and PIN-like 6s ( PIL6s ) and 6 rooting-associated genes root meristem growth factor 9 ( RGF9 ), lateral root primordium 1 ( LRP1s ), dormancy-associated protein homolog 3 ( DRMH3 ). All these identified DEGs were highly up-regulated in certain AR developed stage, indicating their potential roles in blueberry AR formation.Conclusions The transcriptome profiling indicated candidate genes or major regulative factors that influence adventitious root formation in blueberry, and provided a comprehensive understanding of rooting mechanism of the auxin-induced AR formation from blueberry green cuttings.

scholarly journals Comprehensive Transcriptome Analysis Unravels the Existence of Crucial Genes Regulating Primary Metabolism during Adventitious Root Formation in Petunia hybrida

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e100997 ◽  
Author(s):  
Amirhossein Ahkami ◽  
Uwe Scholz ◽  
Burkhard Steuernagel ◽  
Marc Strickert ◽  
Klaus-Thomas Haensch ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Adventitious Root ◽  
Petunia Hybrida ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
Primary Metabolism
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