scholarly journals Plant Virology and Next Generation Sequencing: Experiences with a Potyvirus

PLoS ONE ◽  
2014 ◽  
Vol 9 (8) ◽  
pp. e104580 ◽  
Author(s):  
Monica A. Kehoe ◽  
Brenda A. Coutts ◽  
Bevan J. Buirchell ◽  
Roger A. C. Jones
Keyword(s):  
Next Generation Sequencing ◽  
Next Generation ◽  
Plant Virology ◽  
Generation Sequencing

scholarly journals Next Generation Sequencing Technique and Its Application in Plant Virology

2019 ◽  
Vol 8 (2) ◽  
pp. 77-85
Author(s):  
zohreh Davoodi ◽  
jahangir heydarnejad ◽  
Hossein Masoomi ◽  
◽  
◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Next Generation ◽  
Plant Virology ◽  
Sequencing Technique ◽  
Generation Sequencing

scholarly journals Next-Generation Sequencing and Genome Editing in Plant Virology

2016 ◽  
Vol 7 ◽  
Author(s):  
Ahmed Hadidi ◽  
Ricardo Flores ◽  
Thierry Candresse ◽  
Marina Barba
Keyword(s):  
Next Generation Sequencing ◽  
Genome Editing ◽  
Next Generation ◽  
Plant Virology ◽  
Generation Sequencing

scholarly journals Next-Generation Sequencing and the CRISPR-Cas Nexus: A Molecular Plant Virology Perspective

2021 ◽  
Vol 11 ◽  
Author(s):  
Muhammad Shafiq Shahid ◽  
Muhammad Naeem Sattar ◽  
Zafar Iqbal ◽  
Amir Raza ◽  
Abdullah M. Al-Sadi
Keyword(s):  
Next Generation Sequencing ◽  
Plant Viruses ◽  
Genetically Engineered ◽  
Site Directed Mutagenesis ◽  
Host Susceptibility ◽  
Base Substitution ◽  
Next Generation ◽  
Plant Virology ◽  
Detection Techniques ◽  
Generation Sequencing

In recent years, next-generation sequencing (NGS) and contemporary Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-CRISPR-associated (Cas) technologies have revolutionized the life sciences and the field of plant virology. Both these technologies offer an unparalleled platform for sequencing and deciphering viral metagenomes promptly. Over the past two decades, NGS technologies have improved enormously and have impacted plant virology. NGS has enabled the detection of plant viruses that were previously undetectable by conventional approaches, such as quarantine and archeological plant samples, and has helped to track the evolutionary footprints of viral pathogens. The CRISPR-Cas-based genome editing (GE) and detection techniques have enabled the development of effective approaches to virus resistance. Different versions of CRISPR-Cas have been employed to successfully confer resistance against diverse plant viruses by directly targeting the virus genome or indirectly editing certain host susceptibility factors. Applications of CRISPR-Cas systems include targeted insertion and/or deletion, site-directed mutagenesis, induction/expression/repression of the gene(s), epigenome re-modeling, and SNPs detection. The CRISPR-Cas toolbox has been equipped with precision GE tools to engineer the target genome with and without double-stranded (ds) breaks or donor templates. This technique has also enabled the generation of transgene-free genetically engineered plants, DNA repair, base substitution, prime editing, detection of small molecules, and biosensing in plant virology. This review discusses the utilities, advantages, applications, bottlenecks of NGS, and CRISPR-Cas in plant virology.

scholarly journals Next-generation sequencing and metagenomic analysis: a universal diagnostic tool in plant virology

2009 ◽  
Vol 10 (4) ◽  
pp. 537-545 ◽  
Author(s):  
IAN P. ADAMS ◽  
RACHEL H. GLOVER ◽  
WENDY A. MONGER ◽  
RICK MUMFORD ◽  
ELENA JACKEVICIENE ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Diagnostic Tool ◽  
Metagenomic Analysis ◽  
Next Generation ◽  
Plant Virology ◽  
Generation Sequencing

scholarly journals Next generation sequencing and proteomics in plant virology: how is Colombia doing?

2019 ◽  
Vol 24 (3) ◽  
pp. 423-438
Author(s):  
Johana Madroñero ◽  
Zayda Lorena Corredor Rozo ◽  
Javier Antonio Escobar Pérez ◽  
Myriam Lucia Velandia Romero
Keyword(s):  
Next Generation Sequencing ◽  
Crop Production ◽  
Agricultural Sector ◽  
Negative Impact ◽  
Diagnostic Methods ◽  
Viral Diseases ◽  
Next Generation ◽  
Plant Interactions ◽  
Plant Virology ◽  
Generation Sequencing

Crop production and trade are two of the most economically important activities in Colombia, and viral diseases cause a high negative impact to agricultural sector. Therefore, the detection, diagnosis, control, and management of viral diseases are crucial. Currently, Next-Generation Sequencing (NGS) and ‘Omic’ technologies constitute a right-hand tool for the discovery of novel viruses and for studying virus-plant interactions. This knowledge allows the development of new viral diagnostic methods and the discovery of key components of infectious processes, which could be used to generate plants resistant to viral infections. Globally, crop sciences are advancing in this direction. In this review, advancements in ‘omic’ technologies and their different applications in plant virology in Colombia are discussed. In addition, bioinformatics pipelines and resources for omics data analyses are presented. Due to their decreasing prices, NGS technologies are becoming an affordable and promising means to explore many phytopathologies affecting a wide variety of Colombian crops so as to improve their trade potential.

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