scholarly journals Efficient Induction of Apoptosis by Wee1 Kinase Inhibition in Hepatocellular Carcinoma Cells

PLoS ONE ◽  
2014 ◽  
Vol 9 (6) ◽  
pp. e100495 ◽  
Author(s):  
Tomomi Kogiso ◽  
Hikaru Nagahara ◽  
Etsuko Hashimoto ◽  
Shunichi Ariizumi ◽  
Masakazu Yamamoto ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Carcinoma Cells ◽  
Kinase Inhibition ◽  
Hepatocellular Carcinoma Cells ◽  
Efficient Induction ◽  
Wee1 Kinase ◽  
Induction Of Apoptosis

Rapamycin inhibits cell growth by induction of apoptosis on hepatocellular carcinoma cells in vitro

2007 ◽  
Vol 17 (3) ◽  
pp. 162-168 ◽  
Author(s):  
Jun-Feng Zhang ◽  
Jia-Jun Liu ◽  
Min-Qiang Lu ◽  
Chang-Jie Cai ◽  
Yang Yang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Growth ◽  
Carcinoma Cells ◽  
Hepatocellular Carcinoma Cells ◽  
Induction Of Apoptosis

scholarly journals Ganoderma lucidum Spore Polysaccharide Inhibits the Growth of Hepatocellular Carcinoma Cells by Altering Macrophage Polarity and Induction of Apoptosis

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Ming Song ◽  
Zhen-hao Li ◽  
Hong-shun Gu ◽  
Ru-ying Tang ◽  
Rui Zhang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Flow Cytometry ◽  
Ganoderma Lucidum ◽  
Macrophage Polarization ◽  
Carcinoma Cells ◽  
Inflammatory Factors ◽  
Dependent Manner ◽  
Hepatocellular Carcinoma Cells ◽  
Induction Of Apoptosis ◽  
M1 Type

Background. Ganoderma lucidum has certain components with known pharmacological effects, including strengthening immunity and anti-inflammatory activity. G. lucidum seeds inherit all its biological characteristics. G. lucidum spore polysaccharide (GLSP) is the main active ingredient to enhance these effects. However, its specific biological mechanisms are not exact. Our research is aimed at revealing the specific biological mechanism of GLSP to enhance immunity and inhibit the growth of H22 hepatocellular carcinoma cells. Methods. We extracted primary macrophages (Mø) from BALB/c mice and treated them with GLSP (800 μg/mL, 400 μg/mL, and 200 μg/mL) to observe its effects on macrophage polarization and cytokine secretion. We used GLSP and GLSP-intervened macrophage supernatant to treat H22 tumor cells and observed their effects using MTT and flow cytometry. Moreover, real-time fluorescent quantitative PCR and western blotting were used to observe the effect of GLSP-intervened macrophage supernatant on the PI3K/AKT and mitochondrial apoptosis pathways. Results. In this study, GLSP promoted the polarization of primary macrophages to M1 type and the upregulation of some cytokines such as TNF-α, IL-1β, IL-6, and TGF-β1. The MTT assay revealed that GLSP+Mø at 400 μg/mL and 800 μg/mL significantly inhibited H22 cell proliferation in a dose-dependent manner. Flow cytometry analysis revealed that GLSP+Mø induced apoptosis and cell cycle arrest at the G2/M phase, associated with the expression of critical genes and proteins (PI3K, p-AKT, BCL-2, BAX, and caspase-9) that regulate the PI3K/AKT pathway and apoptosis. GLSP reshapes the tumor microenvironment by activating macrophages, promotes the polarization of primary macrophages to M1 type, and promotes the secretion of various inflammatory factors and cytokines. Conclusion. Therefore, as a natural nutrient, GLSP is a potential agent in hepatocellular carcinoma cell treatment and induction of apoptosis.

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