scholarly journals Tuberculin Skin Testing and Treatment Modulates Interferon-Gamma Release Assay Results for Latent Tuberculosis in Migrants

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e97366 ◽  
Author(s):  
Matthew K. O'Shea ◽  
Thomas E. Fletcher ◽  
Nicholas J. Beeching ◽  
Martin Dedicoat ◽  
David Spence ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Skin Testing ◽  
Latent Tuberculosis ◽  
Interferon Gamma Release Assay ◽  
Tuberculin Skin Testing ◽  
Release Assay

scholarly journals Lessons learned from implementation of interferon-gamma release assay to screen for latent tuberculosis infection in a large multicenter observational cohort study in Brazil

2021 ◽  
Author(s):  
Allyson Guimaraes Costa ◽  
Brenda K.S. Carvalho ◽  
Mariana Araujo-Pereira ◽  
Hiochelson N.S. Ibiapina ◽  
Renata Spener-Gomes ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Tuberculosis Infection ◽  
Observational Research ◽  
Interferon Gamma Release Assay ◽  
Tuberculin Skin Testing ◽  
Ltbi Screening ◽  
Release Assay ◽  
Close Contacts

Background: Interferon-gamma release assay (IGRA) has emerged as a useful tool in identifying latent tuberculosis infection (LTBI). This assay can be performed through testing platforms, such as QuantiFERON-TB Gold Plus (QFT-Plus). This in vitro test has been incorporated by several guidelines worldwide and has recently been considered for the diagnosis of LTBI by the World Health Organization (WHO). The possibility of systematically implementing IGRAs such as QFT-Plus in centers that perform LTBI screening has been accelerated by the decreased availability of tuberculin skin testing (TST) in several countries. Nevertheless, the process to implement IGRA testing in routine clinical care has many gaps. Methods: The study utilized the expertise acquired by the laboratory teams of the Regional Prospective Observational Research in Tuberculosis (RePORT)-Brazil consortium during study protocol implementation of LTBI screening of TB close contacts. Results: RePORT-Brazil includes clinical research sites from Brazilian cities and is the largest multicenter cohort of TB close contacts to date in the country. Operational and logistical challenges faced during IGRA implementation in all four study laboratories are described, as well as the solutions that were developed and led to the successful establishment of IGRA testing in RePORT-Brazil. Conclusions: The problems identified and resolved in this study can assist laboratories implementing IGRAs, in addition to manufacturers of IGRAs providing effective technical support. This will facilitate the implementation of IGRA testing in countries with a high TB burden, such as Brazil.

scholarly journals Diagnosis and Risk Factors of Latent Tuberculosis Infection among Healthcare Workers Using Whole Blood Human Interferon-gamma Release Assay and Tuberculin Skin Testing

2020 ◽  
pp. 15-21
Author(s):  
A. N. Umo ◽  
O. J. Akinjogunla ◽  
N. O. Umoh ◽  
G. E. Uzono
Keyword(s):  
Risk Factors ◽  
Interferon Gamma ◽  
Whole Blood ◽  
Healthcare Workers ◽  
Latent Tuberculosis Infection ◽  
Latent Tuberculosis ◽  
Tuberculosis Infection ◽  
Interferon Gamma Release Assay ◽  
Tuberculin Skin Testing ◽  
Release Assay

This study established the diagnosis and risk factors of latent tuberculosis infection (LTBI) among health-care workers in an endemic population using Tuberculin skin test (TST) and Quantiferon TB-gold. A total of 609 Healthcare workers from tuberculosis treatment facilities in Akwa Ibom State, Nigeria were studied. The Interferon-gamma release assay was performed using 3ml of whole blood by ELISA according to the manufacturer’s instruction (Cellestis Ltd., Carnegie, Australia) after which 0.1 ml of 5 tuberculin units of Purified Protein Derivative (PPD) was administered intra-dermally to each subject. TST results were read after 72 hours by measuring the size of indurations in millimetres. Data were analysed using SPSS version 17 (SPSS Inc., Chicago, Illinois). At the threshold of 10 mm, the prevalence of LTBI by TST was 45.8% and 24.8% at the IGRA diagnostic value of ≥ 0.351 IU. Laboratory staff and ward orderlies as well as being in service for >10 years, were more significantly associated with LTBI. A moderate agreement of 76.7%, k = 0.51 was obtained between TST at 10 mm, and QFT. Neither previous exposure to TST nor BCG vaccination affected the prevalence of LTBI in the study population. The difference of 54% prevalence of LTBI between TST and QFT may be due to non-tuberculous mycobacterium (NTM) since TST is non-specific. This may have grave implications of drug toxicity and development of resistance to anti-TB drug among individuals harbouring NTM, but receiving anti-TB medication. The 76.7% agreement between the two tests is an indication that the 10 mm cut-off induration for TST is still relevant in the diagnosis of LTBI.

Absence of interferon-gamma release assay conversion following tuberculin skin testing

2011 ◽  
Vol 15 (6) ◽  
pp. 767-769 ◽  
Author(s):  
N. Ritz ◽  
C. Yau ◽  
T. G. Connell ◽  
M. Tebruegge ◽  
D. Leslie ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Skin Testing ◽  
Interferon Gamma Release Assay ◽  
Tuberculin Skin Testing ◽  
Release Assay

scholarly journals Interferon Gamma Release Assay versus Tuberculin Skin Testing among Healthcare Workers of Highly Diverse Origin in a Moderate Tuberculosis Burden Country

PLoS ONE ◽  
2016 ◽  
Vol 11 (5) ◽  
pp. e0154803 ◽  
Author(s):  
Sahal Al Hajoj ◽  
Bright Varghese ◽  
Alria Datijan ◽  
Mohammed Shoukri ◽  
Ali Alzahrani ◽  
...  
Keyword(s):  
Interferon Gamma ◽  
Healthcare Workers ◽  
Skin Testing ◽  
Interferon Gamma Release Assay ◽  
Tuberculin Skin Testing ◽  
Release Assay ◽  
Diverse Origin
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